ID A0A0R1ZTQ7_9LACO Unreviewed; 1581 AA.
AC A0A0R1ZTQ7;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=FC18_GL001560 {ECO:0000313|EMBL:KRM55113.1};
OS Lacticaseibacillus sharpeae JCM 1186 = DSM 20505.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lacticaseibacillus.
OX NCBI_TaxID=1291052 {ECO:0000313|EMBL:KRM55113.1, ECO:0000313|Proteomes:UP000051679};
RN [1] {ECO:0000313|EMBL:KRM55113.1, ECO:0000313|Proteomes:UP000051679}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 20505 {ECO:0000313|EMBL:KRM55113.1,
RC ECO:0000313|Proteomes:UP000051679};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRM55113.1}.
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DR EMBL; AYYO01000030; KRM55113.1; -; Genomic_DNA.
DR RefSeq; WP_056975816.1; NZ_AYYO01000030.1.
DR STRING; 1291052.FC18_GL001560; -.
DR PATRIC; fig|1291052.5.peg.1586; -.
DR OrthoDB; 9758243at2; -.
DR Proteomes; UP000051679; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR CDD; cd18785; SF2_C; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR039442; Mrr-like_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR041635; Type_ISP_LLaBIII_C.
DR PANTHER; PTHR47396:SF1; ATP-DEPENDENT HELICASE IRC3-RELATED; 1.
DR PANTHER; PTHR47396; TYPE I RESTRICTION ENZYME ECOKI R PROTEIN; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF13156; Mrr_cat_2; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR Pfam; PF04851; ResIII; 1.
DR Pfam; PF18135; Type_ISP_C; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000313|EMBL:KRM55113.1};
KW Helicase {ECO:0000313|EMBL:KRM55113.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000313|EMBL:KRM55113.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000051679}.
FT DOMAIN 188..399
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 484..662
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1581 AA; 178518 MW; 31EF19626FDD5E8A CRC64;
MRPDQTRPEN NFDSLIAQID ESANGVQRDR GTMFEEVVKS YLLNEPAYKN LYDDVWLLSE
VPAEYHIPKK DLGVDLVARH RDSGELTAVQ AKYYRGKVGK DTINSYVAEL NKNYYADGLL
VTTTDDWNKN AEATLNDGSK DINRIGLSDL KHSSFDWSQF SFGNAEKAIT ASHKQPRAYQ
KEAIDKTVAY FSNHERGKLI MAPGTGKTFT SLKIAEAMAA HEQKQDYFVL YLVPSIQLLS
QTLFSWNSDV TPTTNLVSFA VTSDRNASKK RTYNNNDDSD INIQDIGFPA STNADKLMAN
FKALAPTNGQ RITVIFSTYQ SIDVIHQAQA LGYPEFDLII ADEAHRTTGS HAANEEAGIF
TKVHNNKIVQ ARHRLYQTAT PKIYSQDTKK KGQEENIVIA SMDDVDMYGD EIFRLGFGDA
VAKGILTDYK VEVLAVDEAV IQRNLQSSLA TENGLNIDDI GKIIGVWNAM MKRESFSNKT
AGAPMQRAIA FASVIDNQRG HGAGKVGSKQ IAQEFSHVVN EYLGTDKPNS FHVDVKHVDG
SMNALQKKDA IDWLAADLPD DEARVLSNVK FLTEGIDVPN LDAIIFFAPK RSQIDIVQAV
GRIMRKYKDK EYGYIILPVV VPSGQDPDTV LDDNKTYQAV WQVLNALRSI DERFEASVNK
LDLNKKRPRN LNVIGVGGAP DDGFNRSDGH TGDSHGAEQT ELELNWHEIQ DAIYGRIVKK
VGDRRYLEDW TADVQALAER HIRWIQTLIA DKQSPFAKSF KCYVKSLQHN INSDIDDQQA
IEMLAQHVIT KPIFEALFDQ YSFVNDNPVS RAMEDMIQQM TKAGFDQEQA ALEPFYESVR
MRATGIDNAA AKQQFIVTLY DKFFSTGFSA TTEKLGIVFT PVEIVDFIVK SVDEVLKEHF
GRGLDAENVH ILDPFTGTGT FIARTLEYLN QEMKAGKITL ADITRKYTQE LHANEIVLLS
YYIAAINIES VFDEINGPEN YIPFDGIVLA DTFASTENNS VLDEDMFGGN NKRLQKQQEV
PITAIISNPP YSVGQRNQND DNQNVHYEKL EKRIADTYVK NSTAGLTGGL YDSYIKAFRW
ASDRLNKRGV IGFVSNGAFL DTRSASGLRK SFYDEFNYLY FYNLRGNTRT SGETARKEGG
QIFGSGSRTP ITITILIKDG SDEHHLYYKD IGDYLSRQQK FDAISKAASV NGIEWTELTP
DDNNDWLNQR DKNYQKYIAI SADTPSVFKS NAIGAKTNRD AWLYGFSRTA TYDKAKRLAE
HYNQELDNGT GTPINRDGEY IKWSPKLEQL LKRGQSIKFD EQNIRLGLYR PFTKKYLYYD
NSLVERPGLY YKQFGASNEV IFTTGRGASK DFSALATDLI PNMDCLEKAQ GFMRYNNEEN
NSVENILAPD RDNVSAEFAA GLGLTTDETY AYVYGLLNSP EYQQKYANDL KKDLARIPVV
KYVRKYVDVG QQLLDLHINY EDVEPYADCK ITMHADKPSY RVQKMRFKSR ADHSVIKFNS
DITIADIPER AYDYVVNGRS AIEWIMDQYQ IKTDKASKII DDPNEYSDDP KYIFKLLLRV
VTVSLKTLDL IGTLPQFEVV E
//
