ID A0A0R2D2D3_9LACO Unreviewed; 770 AA.
AC A0A0R2D2D3;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=Kojibiose phosphorylase {ECO:0000313|EMBL:KRM94635.1};
GN ORFNames=FC24_GL000198 {ECO:0000313|EMBL:KRM94635.1};
OS Loigolactobacillus rennini DSM 20253.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Loigolactobacillus.
OX NCBI_TaxID=1423796 {ECO:0000313|EMBL:KRM94635.1, ECO:0000313|Proteomes:UP000051638};
RN [1] {ECO:0000313|EMBL:KRM94635.1, ECO:0000313|Proteomes:UP000051638}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 20253 {ECO:0000313|EMBL:KRM94635.1,
RC ECO:0000313|Proteomes:UP000051638};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 65 family.
CC {ECO:0000256|ARBA:ARBA00006768}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRM94635.1}.
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DR EMBL; AYYI01000087; KRM94635.1; -; Genomic_DNA.
DR RefSeq; WP_057874648.1; NZ_AYYI01000087.1.
DR AlphaFoldDB; A0A0R2D2D3; -.
DR STRING; 1423796.FC24_GL000198; -.
DR PATRIC; fig|1423796.3.peg.207; -.
DR Proteomes; UP000051638; Unassembled WGS sequence.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 2.70.98.40; Glycoside hydrolase, family 65, N-terminal domain; 1.
DR Gene3D; 2.60.420.10; Maltose phosphorylase, domain 3; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR005194; Glyco_hydro_65_C.
DR InterPro; IPR005195; Glyco_hydro_65_M.
DR InterPro; IPR005196; Glyco_hydro_65_N.
DR InterPro; IPR037018; Glyco_hydro_65_N_sf.
DR InterPro; IPR017045; Malt_Pase/Glycosyl_Hdrlase.
DR PANTHER; PTHR11051; GLYCOSYL HYDROLASE-RELATED; 1.
DR PANTHER; PTHR11051:SF13; GLYCOSYL TRANSFERASE-RELATED; 1.
DR Pfam; PF03633; Glyco_hydro_65C; 1.
DR Pfam; PF03632; Glyco_hydro_65m; 1.
DR Pfam; PF03636; Glyco_hydro_65N; 1.
DR PIRSF; PIRSF036289; Glycosyl_hydrolase_malt_phosph; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000051638}.
FT DOMAIN 13..249
FT /note="Glycoside hydrolase family 65 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03636"
FT DOMAIN 322..683
FT /note="Glycoside hydrolase family 65 central catalytic"
FT /evidence="ECO:0000259|Pfam:PF03632"
FT DOMAIN 699..753
FT /note="Glycoside hydrolase family 65 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03633"
FT ACT_SITE 488
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036289-50"
FT BINDING 356..357
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
FT BINDING 596..597
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
SQ SEQUENCE 770 AA; 86762 MW; 5FC48499B547D419 CRC64;
MADEAFQIHL QQLKQKSPGF LETIYALGNG HIGVRASNPL QGNSPAYSGK PGLFVNGFYD
LSPIHYGEPY YGYAHNNQTI CELPDPRYLI FEVDGVRSDK TPYNVETIDK TWDMASGLLD
ETFQITTPTG KTFTLTLDSF VSQAERHLYV VRYDIRALNF SGGIKIFKRH DYVNQQIPRA
TADIRRAQRS NTLMQTYLPA TAPIMELSTQ KSQLGLLVAL RFLGDDPRVT LTTQNDVPCY
QINLDLTPER SAQFTFGYAI GDVHDLLAFK LHQQSNVEAT VALLKEQTFE EAFAASAART
QQFWQDSDIV IEGDSSLQKG IRFNLYHLFQ AAGRDHLTAI PAKGLTGTGY EGHYFWDTEM
YMLPFFIYTQ PEIAKALLGY RYTTLPAAGR RAREMAISAG VLFPWRTING EEASAYYPAG
TAQIHINADI AYATDLYLRA TGDTDFLKSA GFELILNTAR FWLAYGNYAT YGPQRGHFVL
NRVTGPDEYT AMVDNNYYTN RMAKNNLALA VKYADKMAKI APEKLTDLAV TQDERAAFAE
AADKMYLPYD AKHKIKLQDD NAPNRPQFDI AHEPATNFPL LLHYHPLVLY RYQLNKQADT
LMSALLFPLD QDEAQLKRDY DYYEKITTHD SSLSRAVFSI LANRIHQPQK AADYFMDSSL
TDLTDLQGNS EDGIHAANMG GSWLSLVYGF AGMHYGKSGL TFAPHLPTSW QRLKFKIKYR
GRQIAVDLQQ AAITFTLLHG KPLTVYCQQE PCELTLKNPS KQVSLTDSTN
//
