ID A0A0R2FGF0_9LACO Unreviewed; 581 AA.
AC A0A0R2FGF0;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=Alpha-glycerophosphate oxidase {ECO:0000256|ARBA:ARBA00021658};
DE EC=1.1.3.21 {ECO:0000256|ARBA:ARBA00013104};
DE AltName: Full=Glycerol-3-phosphate oxidase {ECO:0000256|ARBA:ARBA00032349};
GN ORFNames=IV36_GL001792 {ECO:0000313|EMBL:KRN26635.1};
OS Liquorilactobacillus mali.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Liquorilactobacillus.
OX NCBI_TaxID=1618 {ECO:0000313|EMBL:KRN26635.1, ECO:0000313|Proteomes:UP000051727};
RN [1] {ECO:0000313|EMBL:KRN26635.1, ECO:0000313|Proteomes:UP000051727}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27304 {ECO:0000313|EMBL:KRN26635.1,
RC ECO:0000313|Proteomes:UP000051727};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=O2 + sn-glycerol 3-phosphate = dihydroxyacetone phosphate +
CC H2O2; Xref=Rhea:RHEA:18369, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57642; EC=1.1.3.21;
CC Evidence={ECO:0000256|ARBA:ARBA00000275};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate
CC dehydrogenase family. {ECO:0000256|ARBA:ARBA00007330}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRN26635.1}.
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DR EMBL; JQAR01000040; KRN26635.1; -; Genomic_DNA.
DR RefSeq; WP_056992568.1; NZ_WERQ01000013.1.
DR AlphaFoldDB; A0A0R2FGF0; -.
DR STRING; 1618.IV36_GL001792; -.
DR PATRIC; fig|1618.3.peg.1824; -.
DR OrthoDB; 9766796at2; -.
DR Proteomes; UP000051727; Unassembled WGS sequence.
DR GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004368; F:glycerol-3-phosphate dehydrogenase (quinone) activity; IEA:InterPro.
DR GO; GO:0004369; F:glycerol-3-phosphate oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IEA:InterPro.
DR Gene3D; 1.10.8.870; Alpha-glycerophosphate oxidase, cap domain; 1.
DR Gene3D; 3.30.9.10; D-Amino Acid Oxidase, subunit A, domain 2; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR031656; DAO_C.
DR InterPro; IPR038299; DAO_C_sf.
DR InterPro; IPR006076; FAD-dep_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000447; G3P_DH_FAD-dep.
DR PANTHER; PTHR11985:SF37; AEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE; 1.
DR PANTHER; PTHR11985; GLYCEROL-3-PHOSPHATE DEHYDROGENASE; 1.
DR Pfam; PF01266; DAO; 1.
DR Pfam; PF16901; DAO_C; 1.
DR PRINTS; PR01001; FADG3PDH.
DR SUPFAM; SSF54373; FAD-linked reductases, C-terminal domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR PROSITE; PS00977; FAD_G3PDH_1; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 21..43
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 21..358
FT /note="FAD dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF01266"
FT DOMAIN 432..553
FT /note="Alpha-glycerophosphate oxidase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16901"
SQ SEQUENCE 581 AA; 63862 MW; B7EFC829BE9B5838 CRC64;
MSFSINSRKS DIQKLKREQQ DLLIIGGGIT GAGVALQASA AGMKTALIDM QDFGGGTSSR
STRLVHGGIR YLKTFDVEVV SDTVKERAVV QHIAPHMVRP DPMLLPIYDE PGTTFTMFSV
KVAMDLYDHL AGINKTPNLK KYANYMLTKE EVLKREPQLN PENLAGGGVY LDYQNNDSRL
VVENIKKAHE KGGLMVSRLK AIKILNDGNG HVIGVKVKDT LTNEEFDIQA KVVINTSGPW
SDFVRHLDED EDKKSQMRPT KGVHLVVDQS RLKVPQPTYF GSGTNDGRMI FTIPREGKTY
FGTTDTDYKG DLTNPRVDMN DVDYLLKIIN KKYPHAHLTL ADVETSWAGV RPLVAAEEPE
TDVVSGASDA GNASAPSAVS RGSSLKVAQD GLITLAGGKL TDYRVMADGA MKKIITILHD
QFDENFEEVN SAELKVSGGD FDSDKAEEIL DSYAKQGVAK GFTPAEAEEI VTLFGSNSLK
IFAAADKATA APGLSLAETL SLHYSLDEEM TLTPVDYLLR RTYHLLFRSE TLDKVKQGVI
NEMARYFAWD QAEVAEQTEL LDKEIAAAKL VDLKHNRSVT N
//
