ID A0A0R2IW42_9LACO Unreviewed; 471 AA.
AC A0A0R2IW42;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=6-phospho-beta-galactosidase {ECO:0000256|RuleBase:RU004469};
DE EC=3.2.1.85 {ECO:0000256|RuleBase:RU004469};
GN ORFNames=IV80_GL001709 {ECO:0000313|EMBL:KRN65869.1};
OS Pediococcus cellicola.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Pediococcus.
OX NCBI_TaxID=319652 {ECO:0000313|EMBL:KRN65869.1, ECO:0000313|Proteomes:UP000051568};
RN [1] {ECO:0000313|EMBL:KRN65869.1, ECO:0000313|Proteomes:UP000051568}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17757 {ECO:0000313|EMBL:KRN65869.1,
RC ECO:0000313|Proteomes:UP000051568};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 6-phospho-beta-D-galactoside + H2O = an alcohol + D-
CC galactose 6-phosphate; Xref=Rhea:RHEA:24568, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:30879, ChEBI:CHEBI:58534, ChEBI:CHEBI:91004; EC=3.2.1.85;
CC Evidence={ECO:0000256|RuleBase:RU004469};
CC -!- PATHWAY: Carbohydrate metabolism; lactose degradation; D-galactose 6-
CC phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
CC {ECO:0000256|RuleBase:RU004469}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC {ECO:0000256|ARBA:ARBA00010838}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRN65869.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JQBR01000007; KRN65869.1; -; Genomic_DNA.
DR RefSeq; WP_057751508.1; NZ_JQBR01000007.1.
DR AlphaFoldDB; A0A0R2IW42; -.
DR STRING; 319652.IV80_GL001709; -.
DR PATRIC; fig|319652.3.peg.1731; -.
DR OrthoDB; 9765195at2; -.
DR UniPathway; UPA00542; UER00605.
DR Proteomes; UP000051568; Unassembled WGS sequence.
DR GO; GO:0033920; F:6-phospho-beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0019512; P:lactose catabolic process via tagatose-6-phosphate; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR005928; 6P-beta-galactosidase.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR018120; Glyco_hydro_1_AS.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01233; lacG; 1.
DR PANTHER; PTHR10353:SF340; 6-PHOSPHO-BETA-GALACTOSIDASE 1; 1.
DR PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU004468};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU004468};
KW Reference proteome {ECO:0000313|Proteomes:UP000051568}.
FT ACT_SITE 377
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10055"
SQ SEQUENCE 471 AA; 53834 MW; E5E17371C29FBD94 CRC64;
MLNRVKSLPK DFVWGGATAA YQVEGATKVG GKGKTMWDDY LKAQGRFSPD PASDFYHCYP
EDVKLAHQYG LNAIRVSIAW TRIFPNGYGE PNQAGVKYYH ELFKACLDQG VTPYVSLHHF
DSPKTLFDQG DWLNRQNIDY FVKYAEFCFK EFTEVKNWFT INELISLSLS QYIAGNFPPN
HHFDVTSAIQ AQHNELLAHA RVVNLYKSLH LDGRIGLIHV LQPIYPITET PENKHAAALY
DAFINRFLLD GTFLGEYSDE TMALINEILE KNGAKLEIQD GDMAILKEAS TQNDFFGLNY
YQSQFIQAYD GPSGNKFNGT GDKGSSSFSF HGVGQIVQKP GVPTTDWDWN IYPEGLYDIL
KRVSKEYPNS KTIYITENGL GYKDNFVSED QVIDDEPRID YIDQHVAALL KARSEGVNVQ
GYFVWSLQDQ FSWANGYNKR YGLFYVDFDT QKRYIKKSAE WFKELADTMH S
//
