ID A0A0R2KCR6_9LACO Unreviewed; 737 AA.
AC A0A0R2KCR6;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=IV43_GL001770 {ECO:0000313|EMBL:KRN87234.1}, LAC1533_0550
GN {ECO:0000313|EMBL:SFV39970.1};
OS Ligilactobacillus acidipiscis.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Ligilactobacillus.
OX NCBI_TaxID=89059 {ECO:0000313|EMBL:KRN87234.1, ECO:0000313|Proteomes:UP000051491};
RN [1] {ECO:0000313|EMBL:KRN87234.1, ECO:0000313|Proteomes:UP000051491}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15353 {ECO:0000313|EMBL:KRN87234.1,
RC ECO:0000313|Proteomes:UP000051491};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
RN [2] {ECO:0000313|Proteomes:UP000190935}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ACA-DC 1533 {ECO:0000313|Proteomes:UP000190935};
RA Papadimitriou K.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:SFV39970.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ACA-DC 1533 {ECO:0000313|EMBL:SFV39970.1};
RA Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family.
CC {ECO:0000256|ARBA:ARBA00006202}.
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DR EMBL; JQBK01000006; KRN87234.1; -; Genomic_DNA.
DR EMBL; LT630287; SFV39970.1; -; Genomic_DNA.
DR RefSeq; WP_010497197.1; NZ_LT630287.1.
DR AlphaFoldDB; A0A0R2KCR6; -.
DR STRING; 89059.LAC1533_0550; -.
DR KEGG; laca:LAC1533_0550; -.
DR PATRIC; fig|89059.3.peg.1887; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000051491; Unassembled WGS sequence.
DR Proteomes; UP000190935; Chromosome i.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536}.
FT DOMAIN 39..293
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 657..733
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 486
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 556
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ SEQUENCE 737 AA; 84075 MW; EE0F755D86CFBEAF CRC64;
MTKPSAKSEL IEVSSNPLTF HLHNSQVSYV LGVEDENILA HHYYGKAVDH YSNLRRYPRL
MRHFAPSPED IETRDFSLST LPQEFPGAGG ADYREPGIVV QFPNGSRTVN FTYDSYTISD
GKPQLAGLPA TFAQEDEAQT LTVKLVESDA QLELYLSYTI FRDLPVITRS ARVKNLGQQP
VQVQRLVSFS LDSPDNELNL LQLNGRYAQE RNVTIEPLNT GIKILDSKRG ASGHTQNPFC
ALIDPHATEF SGEVFGFCLV YSGSHKSIIQ MDGYNQVRIQ MGINDQNFEW WLEPQKSFQS
PEAVLVHSEQ GLNSMSQSFH ELFLNHLMKS KYSHQTRPIL LNNWEATYFN FDEKKLQDII
ASSHDLGIEM FVLDDGWFGE RDAPNSSLGD WVVDRKKLPN GLKPLSDYCH NLDMKFGLWF
EPEMISVKSK LYQSHPDWVL EVPGQRRSID RSQFVLNLGR AEVRDYLLER MSYIIEHADL
DYIKWDMNRN MTEVYDAKLS ASEQGEVSHR YILGLYDLLE RLTDNYPDIL FESCSGGGGR
YDAGMLYYMP QTWTSDNTDA IARLKIQYGT SLVYPVVSMG AHVSAVPNEQ TGRMTPLRTR
GLVASSGNLG YELNPLNLLE NERQEVKLQV KWYKAHRRLI QFGKLYRLQN PFETTRTAWM
FVSDDKKEAV AMYFNIFSEA SSPLHMLNFV GLNPSQKYSV NGEYTAYGSE LMQIGIYPLT
QNDGDFDSAV FELKAVE
//
