ID   A0A0R2X5R1_9FLAO        Unreviewed;       488 AA.
AC   A0A0R2X5R1;
DT   20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT   20-JAN-2016, sequence version 1.
DT   24-JAN-2024, entry version 18.
DE   SubName: Full=Glycosyl hydrolase {ECO:0000313|EMBL:KRP29779.1};
GN   ORFNames=ABS28_03790 {ECO:0000313|EMBL:KRP29779.1};
OS   Cryomorphaceae bacterium BACL22 MAG-120619-bin32.
OC   Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales; Cryomorphaceae.
OX   NCBI_TaxID=1655630 {ECO:0000313|EMBL:KRP29779.1, ECO:0000313|Proteomes:UP000052139};
RN   [1] {ECO:0000313|EMBL:KRP29779.1, ECO:0000313|Proteomes:UP000052139}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BACL3 MAG-120619-bin32 {ECO:0000313|EMBL:KRP29779.1};
RA   Hugerth L.W., Larsson J., Alneberg J., Lindh M.V., Legrand C., Pinhassi J.,
RA   Andersson A.F.;
RT   "Metagenome-Assembled Genomes uncover a global brackish microbiome.";
RL   Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family.
CC       {ECO:0000256|ARBA:ARBA00005382, ECO:0000256|RuleBase:RU361188}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KRP29779.1}.
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DR   EMBL; LIDG01000031; KRP29779.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0R2X5R1; -.
DR   Proteomes; UP000052139; Unassembled WGS sequence.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:InterPro.
DR   GO; GO:0006665; P:sphingolipid metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR033452; GH30_C.
DR   InterPro; IPR001139; Glyco_hydro_30.
DR   InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR11069; GLUCOSYLCERAMIDASE; 1.
DR   PANTHER; PTHR11069:SF23; LYSOSOMAL ACID GLUCOSYLCERAMIDASE; 1.
DR   Pfam; PF02055; Glyco_hydro_30; 1.
DR   Pfam; PF17189; Glyco_hydro_30C; 1.
DR   PRINTS; PR00843; GLHYDRLASE30.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|RuleBase:RU361188};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361188};
KW   Signal {ECO:0000256|ARBA:ARBA00022729}.
FT   DOMAIN          83..422
FT                   /note="Glycosyl hydrolase family 30 TIM-barrel"
FT                   /evidence="ECO:0000259|Pfam:PF02055"
FT   DOMAIN          425..485
FT                   /note="Glycosyl hydrolase family 30 beta sandwich"
FT                   /evidence="ECO:0000259|Pfam:PF17189"
SQ   SEQUENCE   488 AA;  55832 MW;  979E10EF2E535398 CRC64;
     MLFLSFGFYQ CKNFKNNSKS GYQEEQITFD MNSVKATVYT TAHNSNLKLT LTDTISFSEF
     NQPLETEIDI VVDPRNEFQE FIGIGAALTD ASAETFYKLS KENQELFLQA YYDTQKGIGY
     SLARTIIHSC DFSSASYTYV EEGDKDLKSF SIKHDKKYRI PFTKKAIEAA GGKLTMYASP
     WSPPAFMKTN NNMLKGGKLK EEFYQPWANY YAKFIKAYEK EGIPIWGLSI QNEPMAIQRW
     ESCIYTAEEE RDFLKNYLGP TLENEGLGDK KIIVWDHNRD LLYQRASTIL NDPEAAKYVW
     GTGFHWYEDW KDDNPMYENV ARVNEAYPDK KLIFTEGCNE GYDISRIENE DPKLAERYGK
     SMIKDFNNGT VAWTDWNILL DETGGPNHVG NLCFSPVHGN TQTGELTFTN SFYYIGHFSK
     FIRPGAKRIS SVTSSNKIIS TAFKNKNKSI VIIAMNSSNE SLNYSITVDL KTTKLQILPH
     SIQSIVLN
//