ID A0A0R3E688_9BRAD Unreviewed; 906 AA.
AC A0A0R3E688;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=Aconitate hydratase {ECO:0000256|RuleBase:RU361275};
DE Short=Aconitase {ECO:0000256|RuleBase:RU361275};
DE EC=4.2.1.3 {ECO:0000256|RuleBase:RU361275};
GN ORFNames=AOQ71_01110 {ECO:0000313|EMBL:KRQ17616.1};
OS Bradyrhizobium manausense.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=989370 {ECO:0000313|EMBL:KRQ17616.1, ECO:0000313|Proteomes:UP000051936};
RN [1] {ECO:0000313|EMBL:KRQ17616.1, ECO:0000313|Proteomes:UP000051936}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BR3351 {ECO:0000313|EMBL:KRQ17616.1,
RC ECO:0000313|Proteomes:UP000051936};
RA De Araujo J.L., Zilli J.E.;
RT "Draft Genome Sequence of Bradyrhizobium manausense Strain BR 3351T, a
RT Novel Symbiotic Nitrogen-Fixing Alphaproteobacterium Isolated from
RT Brazilian Amazon Rain Forest.";
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the isomerization of citrate to isocitrate via cis-
CC aconitate. {ECO:0000256|RuleBase:RU361275}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501,
CC ECO:0000256|RuleBase:RU361275};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185, ECO:0000256|RuleBase:RU361275}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRQ17616.1}.
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DR EMBL; LJYG01000004; KRQ17616.1; -; Genomic_DNA.
DR RefSeq; WP_057740719.1; NZ_LJYG01000004.1.
DR AlphaFoldDB; A0A0R3E688; -.
DR STRING; 989370.AOQ71_01110; -.
DR OrthoDB; 9764318at2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000051936; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01586; AcnA_IRP; 1.
DR CDD; cd01580; AcnA_IRP_Swivel; 1.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR044137; AcnA_IRP_Swivel.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01341; aconitase_1; 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR PANTHER; PTHR11670:SF54; CYTOPLASMIC ACONITATE HYDRATASE; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|RuleBase:RU361275};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU361275};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|RuleBase:RU361275};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|RuleBase:RU361275};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 77..570
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 700..832
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 906 AA; 98469 MW; 292CAAC4770B1968 CRC64;
MTSLDSFKCK KTLKVGAKTY VYYSLPTAEK NGLKGISKLP YSMKVLLENL LRNEDGRSVK
KEDIVAVSKW LRKKSLEHEI AFRPARVLMQ DFTGVPAVVD LAAMRNAMQK LGGEPEKINP
LVPVDLVIDH SVIVNFFGDN KAFAKNVTEE YKQNQERYEF LKWGQKAFSN FSVVPPGTGI
CHQVNLEYLS QTVWTKKEKM TVGKKTGTFE VAYPDSLVGT DSHTTMVNGL AVLGWGVGGI
EAEACMLGQP LSMLLPNVVG FKLKGALKEG VTATDLVLTV TQMLRKLGVV GKFVEFFGPG
LDNLSVADKA TIANMAPEYG ATCGFFPVDA AAIDYLKTSG RAAPRVALVQ AYAKAQGLFR
TAKSADPVFT ETLTLDLADV VPSMAGPKRP EGRIALPSVA EGFSLALGTE YKKAEEPTKR
FPVEGKDFEI GHGDVVIAAI TSCTNTSNPS VLIGAGLLAR NAAAKGLKAK PWVKTSLAPG
SQVVAGYLAD SGLQKDLDKV GFNLVGFGCT TCIGNSGPLP EEISKSINDN GIVAAAVLSG
NRNFEGRVSP DVQANYLASP PLVVAHALAG SVTKNLAVEP IGEGKDGKPV YLKDIWPTTK
EINAFMKKFV TSTIFKKKYA DVFKGDANWR KIKTVESETY RWNMSSTYVQ NPPYFEGMKK
EPEPVTDIVE ARILAMFGDK ITTDHISPAG SIKLTSPAGK YLSEHQVRPA DFNQYGTRRG
NHEVMMRGTF ANIRIKNFML KGADGNIPEG GLTKHWPDGE QMSIYDAAMK YQQEQVPLVV
FAGAEYGNGS SRDWAAKGTR LLGVRAVICQ SFERIHRSNL VGMGVLPLTF EEGTSWSSLG
LKGDEKVTLR GLVGELKPRQ KLTAEIVSGD GSLQRVSLLC RIDTLDELDY YRNGGILHYV
LRKLAA
//
