ID A0A0S2DHF4_LYSEN Unreviewed; 5579 AA.
AC A0A0S2DHF4;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE SubName: Full=Polyketide synthase pksM {ECO:0000313|EMBL:ALN57843.1};
GN ORFNames=GLE_2494 {ECO:0000313|EMBL:ALN57843.1};
OS Lysobacter enzymogenes.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Lysobacter.
OX NCBI_TaxID=69 {ECO:0000313|EMBL:ALN57843.1, ECO:0000313|Proteomes:UP000061569};
RN [1] {ECO:0000313|EMBL:ALN57843.1, ECO:0000313|Proteomes:UP000061569}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C3 {ECO:0000313|EMBL:ALN57843.1,
RC ECO:0000313|Proteomes:UP000061569};
RA Kobayashi D.Y.;
RT "Genome sequences of Lysobacter enzymogenes strain C3 and Lysobacter
RT antibioticus ATCC 29479.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP
CC synthases family. {ECO:0000256|ARBA:ARBA00008467}.
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DR EMBL; CP013140; ALN57843.1; -; Genomic_DNA.
DR RefSeq; WP_057947581.1; NZ_CP013140.1.
DR STRING; 69.GLE_2494; -.
DR KEGG; lez:GLE_2494; -.
DR PATRIC; fig|69.6.peg.2457; -.
DR OrthoDB; 9808669at2; -.
DR Proteomes; UP000061569; Chromosome.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR GO; GO:0009403; P:toxin biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 1.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 1.10.1240.100; -; 3.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 1.10.1200.10; ACP-like; 6.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 1.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 1.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR Pfam; PF00668; Condensation; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 3.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF08242; Methyltransf_12; 1.
DR Pfam; PF21089; PKS_DH_N; 2.
DR Pfam; PF00550; PP-binding; 6.
DR Pfam; PF14765; PS-DH; 2.
DR SMART; SM00826; PKS_DH; 2.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 6.
DR SMART; SM01294; PKS_PP_betabranch; 5.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR SUPFAM; SSF47336; ACP-like; 6.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 3.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS00455; AMP_BINDING; 1.
DR PROSITE; PS50075; CARRIER; 6.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 3: Inferred from homology;
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000061569};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 961..1040
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1074..1500
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2902..2978
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3026..3099
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3163..3594
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4528..4604
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4658..5111
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 5320..5397
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 5453..5530
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 1049..1071
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2851..2889
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4488..4527
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4623..4658
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5297..5318
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5400..5423
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5579 AA; 588907 MW; 41A3605F9FF7A6A8 CRC64;
MNVRDLLAAF QRGELSRGQL RERLQSLPPP DDDGALSEGQ QGLWMLQCAQ PHGFAYNIPI
CLRLSGPLDP RALRAACIAT LRAHPALASA VVERDSRPRR QAVAADALPF AVHAAEGWSE
HELAQAMQAR LREPFTLERG PWMRADLYSR GSDTGLLLIA VHHIAFDGGS VDAFLHTLCR
AYERAAGASP QDAAPAGEER GFAEFVRWER EFVASEAGDQ ARRYWLQALQ HAGPALGADV
AGPVRPRTLR HALGADAGRA VAAACARLRI TPAAFYLTLY QQLLAAAGGR AGAGVGMAVE
RRPHRDFDAT VGCFVNLIVV PGDREPRAPL AQRLRRTHER LGEALSHADY PFPRVARELR
RAGQGELFDT VFEYKNRGFF ALPALAREWA GVRFEPVEGL YQEGEYPLAF KVAEREDGPV
LYFDHDAARV PEATAEAWLE QLAASIHAAL ASLAEPVAAA AWRALPHTIQ AQARRDPAAL
AAVDADAQLS YGELDARSDA IAAELRRAGV GADAIVGLLL ERSNDALCAL LGVWKSGAAY
LALDPQLPAE RLRFMIEDAG ATTLIVREAQ SRQLPCPVAR TLVLERIAAV AAQERAAPAI
DPGQLAYVVY TSGSTGAPKG VALTHAGLAN LAQAQAAAFG VGAGERVLQF APWSFDASVW
EIAMAWSAGA TLYLAPNGSL QRSDRLAGLM SEARIAVATL PPSALTLLDA TPLPHLRILI
VAGEECTPAL AQRWMPRCRF VNAYGPSETT VCATFKPCDP ERADTFAPGS VPIGRAIANT
DARALDPRTL QPVAAGEVGE LFVAGAALAR GYLGRAALTA ERFVADPYGA PGARMYRTGD
RVRLLADGEL QFVGRADAQV KIRGSRVELG EVEAAAQSHP GVAQCAVALW PQRGQLVAFA
VTGPDAAPDA AALRRHIGER LPDYMVPTRV EFLARLPTTA HGKVDRDALL ARLRDPDLKE
PPAMLRERDI EATVSSILGE VLRLREVPVD QGFFDIGGDS ILAVGAARRI ADAFGIAFDA
THLLDLASVR RIAAHIAAHA VAPGVAQAAV PAQDARHPSA PAPQPRGSEP DRLDDCVAII
GISCQLPGAR DHREFWDNLR EGVESIEFLD PQALRAAGVP ETVLARPGLV AARAAIHDKD
CFDAEFFQVS AQDAQAMDPQ LRLLLQHSWK AVEDAGYRTA AIGDAGVFMS AANAGYHADA
APTPWTLMES SKQYVASAWS QPGSIPTLIS HRLGLRGPSL FVHSNCSSSL VALHTAYRSL
LAGDARHALV GAATLSASES LGYVAERGMN FSSDGHLRPF DADADGMLGG EGVAVVLLKR
AREAIVDGDH VYALLRATAV NNDGADKAGY YAPSAGGQAR VIERVLSDSG VDAESIGYIE
AHGTATRLGD PLELAALGQA FARHTGRRQF CGIGSVKSNL GHLDTAAGLV GCIKVALSLR
HGEIPPSLNY ARPNPAIDFA HSPFYVVDRR TPWPQRETPH RAGVSSFGIG GTNAHALLEQ
FRAPAQAARE PRRCLVPLSA RKPERLRQYA ADLLAFLDRG AGEAALEDIA YTLQVGREPF
ACRLALLAQS VDELREGLRG FLAATSAPAA KPAYDADELA QLLPHWAGSG QWDKIAAAWT
AGADIDWRAL GDAGQARRIA LPTYPFAPTR HGRPTAAPAP ASAPVAVLHP LLHRNDSSLR
RHRYTSVFDG GEFVFADHQV RGARLLPGVA YLEMARAALA QALRSEQAAI TLHNVSWLRP
LIVGPRPLAV HVTVAERASG GYAFQVASTQ ADGAECVHCE GSASLAGADT PAAGAERRLD
FDALRAQCGG AIAGADCYAL FDRLGCRYGP SLRALVDTRA GVDGDGRRFA LGRLRLSAQA
QRDERYVLHP GLLDSALQAS IGLGLDPLSE PEANDASLDG AELEQTLPFS IDRVRITQAP
GDSAWVLVRE AADSSQAVNK LDLSLCDEHG RIAVEIAGLS ARVVSGAGDE IAAATVQTHS
QAPRAAASAA VAVPATAEAQ AETETGLPLG ALPLVPVWDD LGPPEAALLA AEASARRILL
IGADAALRDG LLAACGEAEL RELEPQHSQV EAIVAIRALR PTHVVWIAQG AAAGVAADAQ
VLRCLRLAKA LLREGFEARP LHLLALTAQA LAVRNDECGD PAQAAVHGFL GSLAKEYPHW
SVRACDLDAL DRCPWPALLD AAPDPENSSL ALREGSRYQR RLVPAQLPDS PRSRMVEGGV
YVVLGGAGGL GRAWSEYALR RYRAHIVWLG RREQDETIGA ALRQLSGLGG SVRYLSVDAR
KRSELEAALK QVESEHGVVH GFVHSALTLH DRGVMAVEEA AFADALSSKI ATAAALAGAL
ARYRPADFVL FFSALQSFNP SPGQSSYAAG SAYLDAMATW LRRKGHAGAR TINWGYWGSV
GIVASDAHRR RMLHIGEGSI EPAEAMPVIE AALAGPFDQV ALLKRSAAHG GAALPILRDD
TIQLASAAAG LRFALPDTAL VAAPAAPEPE QSDELDAALA SGLLVQIERF GGDGSGQPPR
LSWPALHARL GKPALYGPWL REALAFLCRT GRLRELGDDE FARGPVSADW AAVERYFAGP
LRDESERAHA ALVAPLLRAL PDILAGRADA TAIMFADASV RRVEDVYKKN PFIEYFNRIV
ASMVAGYAEQ RLRAEPGAPL RVLEIGAGTG ATSAHLFQAL KPHAAQVGEY RYTDVSRAFL
GHAEREYRAL APYLRCQLLD IERPLAAQGI DCGGYDIAVA TNVLHATGDI HLAVRHAKSA
LRRGGLLVIN EMRGHELFAH LTFGLLPGWW AHRDGWLREP GSPGLAPAAW RRVLEEEGLR
AIRFPAADAH RFGQQIIIAE SDGVFRHKRT AVPSREQQPA PAAATPATEP APVSVAAHEP
PPAAMPMHEP APVAATAPVS AARREDVLRF VRSAVAQTLE LTVEDIHADE PFESYGVDSI
LAIRLANALR ARFPDVSSTI VFEHPCVRAL AMHLESLDAG TQTEPSPAAA ATPAVAPVAE
NPAPAPAPAV AVAPAATAPD AGDVRAATIA LVRAVVARTL EQPAEEIDAD EPLENYGVDS
ILAIRIANEL RETFAEVSST VLFEHRSVAA IAAHFLATQP LAAQGLVPAP RKPEPAHAAP
AAAPRAAPAA AYAAPAASVR EAAPPAAAPA PQPASAPRPA AENRAIAIIG VSGRYPRAAD
LDQFWQRISR GEDCVEEIPA ERWPLQGFYE ADPAKAIAEG KSYCKWGGFI DGFADFDPLF
FGIAPREAMA IDPHERLFLQ ETWRAFEHAG YTRDTLASRH QRRVGVFAGI SQSGFERCAS
GQDAGGDAIH PQTSFGSVAN RVSYLFDLQG PSMPIDTMCS SSLTAIHEAC EHLLRGECEV
AAAGGVNLNL HPSRYVASCE GRFLSTSGRC MSFGEGGDGF VPGEGVGVLI LKPLARAVAD
GDPIHAVIRA TAINHGGKAK GYTAPNPAAQ RELVKTAIAK AGIGADDISF VEAHGTGTEL
GDPIEIEALT QAFRASTDAR GFCAIGSVKS NIGHLEAAAG VAGVTKVLLQ LKHRQLAPSL
HAQRVNPHIA FESGPFRLQR ELSPWQAPRA GGRRIASVSS FGAGGANAHL ILEEYAAPAQ
APAASGAPVL VPLSAKDDER LRERARQLLE ACGGLQESQL ADFAYTLQVG REAMESRLAF
VADSLVQVRE KLEGFLQQRK GLEQFHTGQA QRGRNGLSWL REDEDAQALI DTWLARGKLD
RLCEAWVAGA ARVDWERLYP QARPRRIALP TYPFAPERYW IGALPAASTD AARGDAGAGT
PAQAAAYLHP LVHRNTSNLQ GLRFSSRFSG REAVFADHRV AGQRILPAVA YLEMARAAAA
LAAQPGAGAP ASIADVVWLK PVMAGDGPVD LHVHVRPQGE ELVCEFVREE RDAGDGARAA
APCVRASVRL RADAGPAPAQ DLNALRAALV GAPLSGDACY RELAAMGVEY GPSHRGLVEV
QSGADALGAF VLARLHLPAA DAEGCALPPG LLDSAVQAAG FGVADAVHAD NDGAASVPFA
LDRLQWFAAC PDSCWVVVRR ALSAPGAART DIDLCDDDGR VCVRLQGLSG RPYRHGERAA
AAPRTLFFVP DTAGDVAPAD GAHRRLLACD VAAAALDGLA PTADRRALHS AAADPAERFA
AYAAQALTHA QELMDEGGDA PAVLQLAVPE RGAGRLHAAL AALLRQAQAE SSRPLRAQLL
LVDAQADAAS RVRALPLDGA GLREVELSGS QPRADAQEAA TPWREGGVYL IAGGLGGIGA
ALVADIARAH ASAKIVIAGR SDLDAAGLER LKRLSGLGAH VSYRCVDVSH RVQVEELVAE
ILAQHGALHG VLQCAGVAHR RALAALQADE LGQTLTAKVA GTRNLDLATR HLPLDCFVLL
SSLTARTGAD GQAAYATANA FLDEYAQYRS QLQALGERSG RSLSLGLPYW HSEHGMRLPA
QTVEQMRRRG FAALDADTGV AALKRALASP HAQVAVAHGD PALIRAALGT APTDGDGGDR
PTRAARHEAT RETVSAQASE PPAPLAPAAP EDAVAALRAF LSEQLMIAPA RIRAGDGFDA
FGIDSVIAVR LTAALEKNHG PLSKTLFFEY ATVADLAAYL ATRRAPAAQT AAPARIETVS
VQAASTQAAS SEATHERPLA SPIPVPQPTP ARDRDRDRDD IAIIGLAGRY PRSPDLAAFW
DNLKAGVDCV GEIPAERWSL EGFFDPDPAA SGKSYSKWGG FIDGVADFDP RFFNISPREA
PMMDPQERLF LMCSHEAVED AGYTRQALNH GAQAHGGVGV FVGVMHAEYQ LYGAQAQMAG
EPLALGSGIG SVANRVSYFG NYQGPSLAVD TMCSSSLSAI HLACASLREG ECGVAIAGGV
NVSVHPNKYL GLSQARFASG NGRCAAFGEG GDGYVPGEGV GAVVLKPLAR AVADGDRIYA
VLKGSGVNHG GRANGFAVPN AAAQARLIGR TLERSGVDPA AIGYVEAHGT GTALGDPIEI
AGLTRALHER AEAAAGAAEA GEAHAGEPAA LEPGSIRIGS VKSNIGHCES AAGIAGLTKV
LLQLKHATLV PTLHAQRLNP NIDFARTPFA VQRALEPWPA RRRADGTPAP RMAAVSSFGA
GGSNAHLLVE EFLAAAPARD AAPQPHRPAL IVLSASEAER LQDAARRLSA SLAADGYGDA
DLDAIAATLQ TGREHHAHRL GLSARSIAEL ARKLDAFAAG GREGCHYGVA DPERASAPAG
DLPALVERGE HERVLASWVA GTHWPWERLY AQRPCRLSLP TYPFARERYW APDVLRGRVA
SVAGVAAVAP QPVRAPAPAA PAPAVAAPAA PPASVSDPRA ASREVETMLR ETLAEVLYMQ
PGDIALDTAF VDLGMDSVMG VEWLPMIQQR SGVPLGPTKI YEYPTIRELA AYVAGQRPAA
GQGADAPANA QAAESRTAES QPAAPVAAAS QVVADAASHV ATDAPAAPAA APAPLRRIED
VLRETLAEVL YMKPADVGID TAFVDLGMDS VMGVEWLPMI EQRLGVALGP TKIYEYPTIR
DLAGHVLAQS PAPAAATAPA PGSVDAWLQA IYDGKADPAD AQEWLDTAAV DAQGAGHGD
//
