ID A0A0S2SDE4_9GAMM Unreviewed; 314 AA.
AC A0A0S2SDE4;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN Name=rluC {ECO:0000313|EMBL:ALP39741.1};
GN ORFNames=ATO46_07300 {ECO:0000313|EMBL:KUE79095.1}, WL1483_322
GN {ECO:0000313|EMBL:ALP39741.1};
OS Aeromonas schubertii.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Aeromonas.
OX NCBI_TaxID=652 {ECO:0000313|EMBL:ALP39741.1, ECO:0000313|Proteomes:UP000058114};
RN [1] {ECO:0000313|Proteomes:UP000058114}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WL1483 {ECO:0000313|Proteomes:UP000058114};
RA Liu L.;
RT "Complete Genome Sequence of Aeromonas schubertii strain WL1483.";
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KUE79095.1, ECO:0000313|Proteomes:UP000054876}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43700 {ECO:0000313|EMBL:KUE79095.1,
RC ECO:0000313|Proteomes:UP000054876};
RA Liu L., Li Q., Zhang F., Shi B.;
RT "Complete genome sequence of Aeromonas schubertii strain ATCC43700.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:ALP39741.1, ECO:0000313|Proteomes:UP000058114}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WL1483 {ECO:0000313|EMBL:ALP39741.1,
RC ECO:0000313|Proteomes:UP000058114};
RX PubMed=26798095;
RA Liu L., Li N., Zhang D., Fu X., Shi C., Lin Q., Hao G.;
RT "Complete Genome Sequence of the Highly Virulent Aeromonas schubertii
RT Strain WL1483, Isolated from Diseased Snakehead Fish (Channa argus) in
RT China.";
RL Genome Announc. 4:e01567-15(2016).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 955, 2504 and 2580 in 23S ribosomal RNA.
CC {ECO:0000256|ARBA:ARBA00002876}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|RuleBase:RU362028};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(955/2504/2580) in 23S rRNA =
CC pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.24;
CC Evidence={ECO:0000256|ARBA:ARBA00000381};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
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DR EMBL; CP013067; ALP39741.1; -; Genomic_DNA.
DR EMBL; LPUO01000034; KUE79095.1; -; Genomic_DNA.
DR RefSeq; WP_050664838.1; NZ_LPUO01000034.1.
DR STRING; 652.WL1483_322; -.
DR KEGG; asr:WL1483_322; -.
DR PATRIC; fig|652.5.peg.2188; -.
DR OrthoDB; 9807829at2; -.
DR Proteomes; UP000054876; Unassembled WGS sequence.
DR Proteomes; UP000058114; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF93; PSEUDOURIDYLATE SYNTHASE RPUSD4, MITOCHONDRIAL; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|RuleBase:RU362028};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 20..79
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
FT ACT_SITE 143
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 314 AA; 35750 MW; 23864A0CAD32084A CRC64;
MTQITQQVQL LTIEAEHEGQ RIDNFLKTQL KGVPKSLIYR ILRKGEVRVN KKRVKPEYKL
ETGDEVRVPP LRVAEKNELP SANLGSIQRL EGQILFEDDA IIVLNKPSGM AVHGGSGLSF
GVIEGLRALR PEARFMELVH RLDRDTSGVL LIAKKRSALR SLHEQLRVKT MRKQYLALVR
GCWQSHVKVI TAPLKKNELQ SGERIVRVSA EGKPSETRFR IVRQFAEATL MECSPITGRT
HQIRVHTQHA GHPIACDDKY GERQFDEKMR EKGLNRLFLH AWRLTFAHPA DGREIKVEAP
LGESLDAFLN TLER
//
