ID   A0A0S3QX93_PHAAN        Unreviewed;       790 AA.
AC   A0A0S3QX93;
DT   17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT   17-FEB-2016, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   RecName: Full=Glycosyl hydrolase family 13 catalytic domain-containing protein {ECO:0000259|SMART:SM00642};
GN   Name=Vigan.01G037800 {ECO:0000313|EMBL:BAT72931.1};
GN   ORFNames=VIGAN_01037800 {ECO:0000313|EMBL:BAT72931.1};
OS   Vigna angularis var. angularis.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX   NCBI_TaxID=157739 {ECO:0000313|EMBL:BAT72931.1, ECO:0000313|Proteomes:UP000291084};
RN   [1] {ECO:0000313|EMBL:BAT72931.1, ECO:0000313|Proteomes:UP000291084}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Shumari {ECO:0000313|Proteomes:UP000291084};
RX   PubMed=26616024; DOI=10.1038/srep16780;
RA   Sakai H., Naito K., Ogiso-Tanaka E., Takahashi Y., Iseki K., Muto C.,
RA   Satou K., Teruya K., Shiroma A., Shimoji M., Hirano T., Itoh T., Kaga A.,
RA   Tomooka N.;
RT   "The power of single molecule real-time sequencing technology in the de
RT   novo assembly of a eukaryotic genome.";
RL   Sci. Rep. 5:16780-16780(2015).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000256|ARBA:ARBA00004229}.
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DR   EMBL; AP015034; BAT72931.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0S3QX93; -.
DR   Proteomes; UP000291084; Chromosome 1.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0019156; F:isoamylase activity; IEA:UniProt.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0043170; P:macromolecule metabolic process; IEA:UniProt.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR048650; ISOA1-3-like_C.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF21156; ISOA1-3_C; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW   Plastid {ECO:0000256|ARBA:ARBA00022640};
KW   Reference proteome {ECO:0000313|Proteomes:UP000291084};
KW   Signal {ECO:0000256|SAM:SignalP};
KW   Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           16..790
FT                   /note="Glycosyl hydrolase family 13 catalytic domain-
FT                   containing protein"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5012565538"
FT   DOMAIN          228..654
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   790 AA;  88916 MW;  E3D61E0AEF22119D CRC64;
     MKCFSLPSLS VPTLAVLPQC AVTVRVSLIN RFSQLHESDS VIRSVAKTRA ARNGGAFDTE
     IAVVEKPQLE SLFQVSSGYP SPFGATVRDG GVNFAIYSLN ALSATLCLFT LSDFQNNRVT
     ESVPLDPLIN KTGGVWHVFL KGDFSDMLYG YQFDGKFSPL EGHYYDSSRI LLDPYAKAVI
     SRGEFGALGP NGNCWPQMAG MVPSDHDEFD WEGDLPLKYP QKDLVVYEMH VRGFTKHESS
     NTKFPGTYLG VVEKLDHLKE LGVNCLELMP CHEFNELEYS SYNSVQGDYR VNFWGYSTVN
     FFSPMIRYSS AGIRNCGRDG INEIKFLIKE AHKRGIEVIM DVVFNHTAEG NENGPIISFR
     GVDNSIYYMV APKGEFYNYS GCGNTFNCSH PVVRQFIVDC LRYWVTEMHV DGFRFDLASI
     MTRSSSLWDA TNVFGAPIEG DFLTTGTPLG SPPLIDLISN DPILRGVKLI AEAWDAGGLY
     QVGTFPHWGI WSEWNGKYRD TVRQFIKGTD GFAGAFAECL CGSPNLYQGG GRKPWNSINF
     VCAHDGFTLA DLVTYNNKHN LSNGEDNNDG ENHNNSWNCG QEGEFVSPLV KKLRKRQMRN
     FFLTLMVSQG VPMIYMGDEY GHTKGGNNNT YCHDNYLNYF QWDKKEESSS DFFRFCCLVT
     KFRQECESMG LDDFPTSARL QWHGHFPGMP DWSETSRFVA FTMVDSVKGE IYVAFNTSHL
     PFTITLPDRP GYRWEPLVDT SKAAPYDFLT PDLPGREIAI QQYAQFLDAN MYPMLSYSSV
     ILLRSPDQNA
//