ID A0A0S4LJ70_9BACT Unreviewed; 514 AA.
AC A0A0S4LJ70;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=Cobalamin B12-binding/Radical SAM family protein {ECO:0000313|EMBL:CUS36969.1};
GN ORFNames=COMA2_30009 {ECO:0000313|EMBL:CUS36969.1};
OS Candidatus Nitrospira nitrificans.
OC Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
OC Nitrospira.
OX NCBI_TaxID=1742973 {ECO:0000313|EMBL:CUS36969.1, ECO:0000313|Proteomes:UP000198736};
RN [1] {ECO:0000313|Proteomes:UP000198736}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Luecker S., Luecker S.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; CZPZ01000023; CUS36969.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S4LJ70; -.
DR STRING; 1742973.COMA2_30009; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000198736; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR027564; HpnR_B12_rSAM.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR04367; HpnR_B12_rSAM; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF17; MG-PROTOPORPHYRIN IX MONOMETHYL ESTER OXIDATIVE CYCLASE 66KD SUBUNIT; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDF00565; hopanoid_C3-methyltransferase; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000198736};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 8..141
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 182..396
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 514 AA; 58516 MW; 18056B51A7B215FA CRC64;
MKFLAVHPGP LMYTKIYLRL EPLGLEMVAQ AVRQAGHEVR LIDLQADTWK DYLALITAWK
PDAVAFGCNY LANVPEIVDL AKLTKKELPD CFVFVGGHSA SFVAKDFLEH GNGAIDCVLK
GEGEVAAPKL LMAVEEDRKA ITKVPGVVTL DGEGPPPQFI ENLDDVRPAR DLLRNRHKYF
IGVLDPCASV EFARGCPWDC SFCSAWTFYG RSYRMVSPEK AVEELEQVQE PGIFLVDDVA
FIQGKQGMEI GEAVARRGIK KQYYMETRGD VLLRNKEVFQ FWKTLGLQYM FLGVEAIDAE
GLKMHRKRIS LGRNFEALEF ARSLGITVAI NLIADPDWDR HRFEVIRQWC LEIPEIVNIS
VNTPYPGTES WLTESRKLHT RDYRLFDIQH AVMPTKMPLH EFYAELVKTQ QVLNKKHLGW
AALKGTAKIA AGHLMRGQTN FIKMLWKFNS VYNPELQMAD HRRSVTYEMT MPPEKKDKID
PKQLFILPPK GRHSRAIDDA TETFVDETRM GTVV
//
