ID A0A0S4LQT2_9BACT Unreviewed; 1156 AA.
AC A0A0S4LQT2;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:CUS39321.1};
GN ORFNames=COMA2_70079 {ECO:0000313|EMBL:CUS39321.1};
OS Candidatus Nitrospira nitrificans.
OC Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
OC Nitrospira.
OX NCBI_TaxID=1742973 {ECO:0000313|EMBL:CUS39321.1, ECO:0000313|Proteomes:UP000198736};
RN [1] {ECO:0000313|Proteomes:UP000198736}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Luecker S., Luecker S.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR EMBL; CZPZ01000034; CUS39321.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S4LQT2; -.
DR STRING; 1742973.COMA2_70079; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000198736; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000198736}.
FT DOMAIN 619..780
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 801..955
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1156 AA; 129279 MW; 7A3A814F6DAB86C9 CRC64;
MSEAQIQPPA WLVPLRSALS QGQARACLLG AHGSTAACAL TLLNDIPQAT PDRAGLCVVV
TPNDDSAERI FNDLCFFHGL IGRPVEDLAW FPEWETLPYE ATAPHVGLIA HRMTTLHRLL
INPPSILVTS VAAAMHRVIP RSTFEQAIFH FQTGAAFERE SLITNLLRLG YRRVSVVEIP
GEFSVRGGII DIFSTAYANP VRVEFLGDHV ESIRLFDAST QTSITKLNEG WVLPAREFVR
PPHAPEAIVP IQADAEWRSP DLYDSMDTLF DYLTAVPFLA LDQLDALKKA CETAWEKIDD
GYLRHVDRDA TQPYPSPERL FLTWEGIQQR IAPWPMLALE PLAAPDATWD QTFVFSAQTP
GSIGLGIRGT AFSQTLSLLE GLRNEHRVVL VARSRGQVDR LLALLREHDL PADPWNPTAW
SSQRTGKRPF YVLHGDLSAG FLSGDFRLAL LTEEELFAKG ARHKPQPKSR TATFLSSLED
LNVGDYVVHV QHGIAKYRGL KRLSVQDFES DFLILEFSGG DTLYVPLDRL NQVQRYGGAE
GHVPRLDRLG GTSWAKTTAR VKKNIEEMAQ ELIDLYANRE LVKRNAYGST TTLYHEFEAA
FEYEETSDQL RAIADIGRDM ESTKPMDRLV CGDVGYGKTE VAMRAAFKAV EHDRQVAVLV
PTTLLAHQHY ENFAERFAPF PMRVALLSRF QSPKEAKATL KDVGAGTVDI VIGTHRLLQK
DVAFRQLGLV IIDEEQWFGV KHKERLKQLR TQVDVLTLTA TPIPRTLQMA MSSVRDLSII
DTPPAGRLAI KTEVVRFSDK AVRDAILREL GRGGQIYFVH NRVETMERIG AWLHQLVPEA
RMVMAHGQMD ARPLEAVMLK FVKREADVLI ASAIIQSGLD VPNANTIIVN RADLFGLAQL
YQLRGRVGRG GEQAYAYFLI PDEGTLTGDA QKRLIAIQQF TELGSGFRIA AADLEIRGAG
NLLGKQQSGH IAAIGLDLYM QMVEQAVQRL KGHVIEEDPD PTLQFPVSAF IPEQYVTDPH
HRLSLYKRLT ACGQIGELAL LHGEIQDRYG PPPEPVERLL EVMQLQTHAK RLRLASIEVH
DQTTKVVFHP KAVIPESSVH RLMDQLKKRL RLLSPVAFEI QMRHSDWPSL FSELNAILQS
LDLCDTKTIQ RDTTVS
//
