ID   A0A0S6X031_9SPHN        Unreviewed;       673 AA.
AC   A0A0S6X031;
DT   17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT   17-FEB-2016, sequence version 1.
DT   27-MAR-2024, entry version 22.
DE   SubName: Full=Malate dehydrogenase {ECO:0000313|EMBL:GAM04710.1};
GN   ORFNames=MBENS4_1708 {ECO:0000313|EMBL:GAM04710.1};
OS   Novosphingobium sp. MBES04.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Novosphingobium.
OX   NCBI_TaxID=1206458 {ECO:0000313|EMBL:GAM04710.1};
RN   [1] {ECO:0000313|EMBL:GAM04710.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MBES04 {ECO:0000313|EMBL:GAM04710.1};
RA   Ohta Y., Nishi S., Kobayashi K., Tsubouchi T., Iida K., Tanizaki A.,
RA   Kurosawa K., Adachi A., Nishihara M., Sato R., Hasegawa R., Hatada Y.;
RT   "Draft Genome Sequence of Novosphingobium sp. Strain MBES04, Isolated from
RT   Sunken Wood from Suruga Bay, Japan.";
RL   Genome Announc. 3:e01373-14(2015).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|ARBA:ARBA00001936};
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DR   EMBL; DF850488; GAM04710.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0S6X031; -.
DR   STRING; 1206458.MBENS4_1708; -.
DR   HOGENOM; CLU_012366_2_0_5; -.
DR   Proteomes; UP000053513; Unassembled WGS sequence.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR   GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR   Gene3D; 3.40.50.10950; -; 1.
DR   Gene3D; 3.40.50.10750; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR   Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N_dom.
DR   InterPro; IPR037062; Malic_N_dom_sf.
DR   InterPro; IPR012302; Malic_NAD-bd.
DR   InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR042113; P_AcTrfase_dom1.
DR   InterPro; IPR042112; P_AcTrfase_dom2.
DR   InterPro; IPR002505; PTA_PTB.
DR   PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR   PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   Pfam; PF01515; PTA_PTB; 1.
DR   SMART; SM01274; malic; 1.
DR   SMART; SM00919; Malic_M; 1.
DR   SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR   SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   4: Predicted;
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT   DOMAIN          1..73
FT                   /note="Malic enzyme N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM01274"
FT   DOMAIN          85..322
FT                   /note="Malic enzyme NAD-binding"
FT                   /evidence="ECO:0000259|SMART:SM00919"
SQ   SEQUENCE   673 AA;  72761 MW;  91E771EB21C369CC CRC64;
     MGNLGALASK PVMEGKAVLF KRFADVDSID IELNSEDPEA LIEAIAMMEP SFGGINLEDI
     KAPECFVIEQ TLRDRMNIPV MHDDQHGTAI ITAAGLINAC LITGRKIDEI KVVVNGAGAA
     ALACTSLIKS MGVRHDNVII CDRSGTIYQG RTEQMDQWKS AHAANTEART LEEALVGADV
     FLGLSAAGAL PPEYVKKMAD EPIIFAMANP VPEINPDEAK AVRPDAIIAT GRSDFPNQVN
     NVLGFPFIFR GALDVSATAI NEEMKIAAAT AIAELAREQV HEDVAAAYGG AVQQFGRDYI
     IPAPFDPRLM EVVPAAVAKA AMDSGVAQRP IEDFDAYRLQ LKGRLNPTTS VLSSVYAQVR
     ANPKRVIFAE ADNEVVLRAA IQYRDFGYGE PVLVGRTQNI LDLMGELGVP NPESFHIENS
     MVSDHVAPMV EMIYARLKRR GYLERDVQRM VNTDRNTFAS GLLKMGVGDA MITGMTRPFA
     QSMKNVRRVL DPAPDKLPFG MHMLVGKNRT VFLADTTINE RPSAEELAII ATEAASVAKR
     MGHEPRVAFL SYSTFGNPEG QWVERTRDAV HILDERDDVP FEYEGEMAPD AALNPKIMAL
     YPFCRLSAPA NVLIMPGLQS ANISAKLLRE IGGTTSIGPI LLGMEKPVQI VPMTAIAPDV
     LTSAVLAAAG IAG
//