ID A0A0S7YET3_9BACT Unreviewed; 516 AA.
AC A0A0S7YET3;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=Radical SAM core domain-containing protein {ECO:0000259|PROSITE:PS51918};
GN ORFNames=AMJ48_02170 {ECO:0000313|EMBL:KPJ73246.1};
OS Parcubacteria bacterium DG_74_1.
OC Bacteria; Candidatus Parcubacteria.
OX NCBI_TaxID=1703765 {ECO:0000313|EMBL:KPJ73246.1, ECO:0000313|Proteomes:UP000051540};
RN [1] {ECO:0000313|EMBL:KPJ73246.1, ECO:0000313|Proteomes:UP000051540}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DG_74_1 {ECO:0000313|EMBL:KPJ73246.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|PIRSR:PIRSR005669-1};
CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine.
CC {ECO:0000256|PIRSR:PIRSR005669-1};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPJ73246.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LJNE01000006; KPJ73246.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S7YET3; -.
DR STRING; 1703765.AMJ48_02170; -.
DR PATRIC; fig|1703765.3.peg.606; -.
DR Proteomes; UP000051540; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.40.630.30; -; 1.
DR InterPro; IPR039661; ELP3.
DR InterPro; IPR034687; ELP3-like.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR032432; Radical_SAM_C.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR01211; ELP3; 1.
DR PANTHER; PTHR11135:SF0; ELONGATOR COMPLEX PROTEIN 3; 1.
DR PANTHER; PTHR11135; HISTONE ACETYLTRANSFERASE-RELATED; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF16199; Radical_SAM_C; 1.
DR PIRSF; PIRSF005669; Hist_AcTrfase_ELP3; 1.
DR SFLD; SFLDF00344; ELP3-like; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Iron {ECO:0000256|PIRSR:PIRSR005669-1};
KW Iron-sulfur {ECO:0000256|PIRSR:PIRSR005669-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR005669-1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 74..351
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT BINDING 89
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|PIRSR:PIRSR005669-1"
FT BINDING 93
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|PIRSR:PIRSR005669-1"
FT BINDING 96
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|PIRSR:PIRSR005669-1"
SQ SEQUENCE 516 AA; 60152 MW; 1F3F53CE555B8686 CRC64;
MEILQQIIKD LIKGQIKTST ELVSFKRRVS KKYKIPCPSN IKLLRAYHEL VKNKSIKKSM
ELEDTLKTRP IRSLSGVVNV SVLTKPYPCP GKCLYCPIEK GIPKSYVSGE PAVERAKKLK
YNPCSQVKKR IEMLGIEGHP TDKIEIRIVG GTWSYYPRDY QAWFIKRCSD ACNEKPSKNL
KEAQKLNEKA KHRIVGLSVE TRPDFINKNE VKRLRDLGVT MVEIGVQSIY DNILKLNQRG
HGVRETILAT KLLKDAGFKV LYQIMPNLLG STLKRDKKMF KELFSNSDFQ PDLLKIYPCA
LLKETPLYEL WKKGKYKPYA QSQLINLLKS IKKKMPYYVR IQRITRDIPS KIIIVGPAKI
SNLRQILFQK MRKERWQCKC IRCREVKEAY NQKEKIYFFR QDYKASGGRE VFLSFESKQK
TKLYSLLRLH LTSDKRAIIR ELHTYGQQLS FLYPLKKLAV SPQHKGLGKK LVKEAEKITK
KDFKLNKIAV ISAVGTRNYF RRLGYRLKDT YMVKKI
//
