ID A0A0S7Z723_9GAMM Unreviewed; 467 AA.
AC A0A0S7Z723;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=Tyrosine phenol-lyase {ECO:0000256|ARBA:ARBA00016166, ECO:0000256|HAMAP-Rule:MF_00543};
DE EC=4.1.99.2 {ECO:0000256|ARBA:ARBA00013154, ECO:0000256|HAMAP-Rule:MF_00543};
DE AltName: Full=Beta-tyrosinase {ECO:0000256|ARBA:ARBA00033449, ECO:0000256|HAMAP-Rule:MF_00543};
GN Name=tpl {ECO:0000256|HAMAP-Rule:MF_00543};
GN ORFNames=AMJ58_01240 {ECO:0000313|EMBL:KPJ82384.1};
OS Gammaproteobacteria bacterium SG8_30.
OC Bacteria; Pseudomonadota; Gammaproteobacteria.
OX NCBI_TaxID=1703404 {ECO:0000313|EMBL:KPJ82384.1, ECO:0000313|Proteomes:UP000053683};
RN [1] {ECO:0000313|EMBL:KPJ82384.1, ECO:0000313|Proteomes:UP000053683}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SG8_30 {ECO:0000313|EMBL:KPJ82384.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-tyrosine = NH4(+) + phenol + pyruvate;
CC Xref=Rhea:RHEA:21704, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15882, ChEBI:CHEBI:28938, ChEBI:CHEBI:58315; EC=4.1.99.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001821, ECO:0000256|HAMAP-
CC Rule:MF_00543};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000256|ARBA:ARBA00001933,
CC ECO:0000256|HAMAP-Rule:MF_00543, ECO:0000256|PIRSR:PIRSR611166-50};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881,
CC ECO:0000256|HAMAP-Rule:MF_00543}.
CC -!- SIMILARITY: Belongs to the beta-eliminating lyase family.
CC {ECO:0000256|ARBA:ARBA00009721, ECO:0000256|HAMAP-Rule:MF_00543}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPJ82384.1}.
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DR EMBL; LJNS01000004; KPJ82384.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S7Z723; -.
DR STRING; 1703404.AMJ58_01240; -.
DR PATRIC; fig|1703404.3.peg.1062; -.
DR Proteomes; UP000053683; Unassembled WGS sequence.
DR GO; GO:0050371; F:tyrosine phenol-lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006570; P:tyrosine metabolic process; IEA:InterPro.
DR CDD; cd00617; Tnase_like; 1.
DR Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR HAMAP; MF_00543; Tyr_phenol_lyase; 1.
DR InterPro; IPR001597; ArAA_b-elim_lyase/Thr_aldolase.
DR InterPro; IPR011166; Beta-eliminating_lyase.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR InterPro; IPR013441; Tyr_phenol_ly.
DR NCBIfam; TIGR02618; tyr_phenol_ly; 1.
DR PANTHER; PTHR32325; BETA-ELIMINATING LYASE-LIKE PROTEIN-RELATED; 1.
DR PANTHER; PTHR32325:SF4; TRYPTOPHANASE; 1.
DR Pfam; PF01212; Beta_elim_lyase; 1.
DR PIRSF; PIRSF001386; Trpase; 1.
DR SUPFAM; SSF53383; PLP-dependent transferases; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|HAMAP-Rule:MF_00543, ECO:0000313|EMBL:KPJ82384.1};
KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898, ECO:0000256|HAMAP-
KW Rule:MF_00543}.
FT DOMAIN 57..433
FT /note="Aromatic amino acid beta-eliminating lyase/threonine
FT aldolase"
FT /evidence="ECO:0000259|Pfam:PF01212"
FT MOD_RES 268
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00543,
FT ECO:0000256|PIRSR:PIRSR611166-50"
SQ SEQUENCE 467 AA; 52109 MW; A31A81D574097C94 CRC64;
MAKEPQGRRP TRRSWAEPWK VKVVEPIKML SPAERERAIA EAGYNTFLLR SEDVYIDLLT
DSGTSAMSDW QWAGMMMGDE AYAGSRNFYH LEAAIREHYG YRYVVPTHQG RGAENLLSRI
LIEPGDFVPG NMYFTTTRLH QELAGGTFVD VIVPEAHDPA DESPFKGNVD LEALEALIRK
VGADRIPYVC IAGTVNMAGG QPMSMANARA VRELTARHGI RVMLDATRAV ENAYFIQQRE
PGYRDKPVAE ILREYCSCTD GCTMSGKKDA LVNIGGWLAV NERELYEEAS NLVVVYEGLH
TYGGMAGRDM EAMARGIVES VDDDHIHARV GQVEYLGHKL IDLGVPVVRP IGGHAVYLDA
RAFYPQIRQD DFPAQTLAAS LYADSGVRAM ERGIVSAGRD AETGEHHYPA LELVRLTIPR
RVYTQAHMDL TAESVAAVFD ARESARGLKM TYEPKYLRFF QARFEPL
//
