ID A0A0S8AC64_9GAMM Unreviewed; 424 AA.
AC A0A0S8AC64;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=DNA-binding transcriptional regulator NtrC {ECO:0000256|ARBA:ARBA00019059, ECO:0000256|RuleBase:RU365013};
DE AltName: Full=Nitrogen regulation protein NR(I) {ECO:0000256|RuleBase:RU365013};
DE Flags: Fragment;
GN Name=glnG {ECO:0000313|EMBL:KPJ96964.1};
GN Synonyms=ntrC {ECO:0000256|RuleBase:RU365013};
GN ORFNames=AMJ55_00980 {ECO:0000313|EMBL:KPJ96964.1};
OS Gammaproteobacteria bacterium SG8_15.
OC Bacteria; Pseudomonadota; Gammaproteobacteria.
OX NCBI_TaxID=1703403 {ECO:0000313|EMBL:KPJ96964.1, ECO:0000313|Proteomes:UP000053644};
RN [1] {ECO:0000313|EMBL:KPJ96964.1, ECO:0000313|Proteomes:UP000053644}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SG8_15 {ECO:0000313|EMBL:KPJ96964.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- FUNCTION: Member of the two-component regulatory system NtrB/NtrC,
CC which controls expression of the nitrogen-regulated (ntr) genes in
CC response to nitrogen limitation. Phosphorylated NtrC binds directly to
CC DNA and stimulates the formation of open promoter-sigma54-RNA
CC polymerase complexes. {ECO:0000256|RuleBase:RU365013}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|RuleBase:RU365013}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPJ96964.1}.
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DR EMBL; LJNL01000009; KPJ96964.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S8AC64; -.
DR Proteomes; UP000053644; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006808; P:regulation of nitrogen utilization; IEA:InterPro.
DR CDD; cd00009; AAA; 1.
DR CDD; cd19919; REC_NtrC; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.40.50.2300; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR InterPro; IPR010114; Transcript_reg_NtrC.
DR NCBIfam; TIGR01818; ntrC; 1.
DR PANTHER; PTHR32071:SF15; DNA-BINDING TRANSCRIPTIONAL REGULATOR NTRC; 1.
DR PANTHER; PTHR32071; TRANSCRIPTIONAL REGULATORY PROTEIN; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00158; Sigma54_activat; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF52172; CheY-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 4: Predicted;
KW Activator {ECO:0000256|RuleBase:RU365013};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU365013};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|RuleBase:RU365013};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU365013};
KW Nitrogen fixation {ECO:0000256|RuleBase:RU365013};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU365013};
KW Phosphoprotein {ECO:0000256|PROSITE-ProRule:PRU00169};
KW Repressor {ECO:0000256|RuleBase:RU365013};
KW Transcription {ECO:0000256|RuleBase:RU365013};
KW Transcription regulation {ECO:0000256|RuleBase:RU365013};
KW Two-component regulatory system {ECO:0000256|RuleBase:RU365013}.
FT DOMAIN 17..131
FT /note="Response regulatory"
FT /evidence="ECO:0000259|PROSITE:PS50110"
FT DOMAIN 152..381
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000259|PROSITE:PS50045"
FT COILED 335..362
FT /evidence="ECO:0000256|SAM:Coils"
FT MOD_RES 66
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00169"
FT NON_TER 424
FT /evidence="ECO:0000313|EMBL:KPJ96964.1"
SQ SEQUENCE 424 AA; 47415 MW; 61D66CC761F9E72C CRC64;
MNNTAATNIK HPLTTTSVWV VDDDRSIRWV LERALKQAGM EVETFENAKK VLQEIGKRQP
NAIITDIRMP GIDGLALLNK LNQQAPGLPV IIMTAHSDLD SAVSAYQGGA FEYLPKPFDV
DDAIELVNRA ITHSLNQQDA APAVENSETP EIIGEAASMQ EVFRAIGRLS RSKITVLITG
QSGTGKELVA KALHKHSPRA DKPFIALNTA AIPKDLLESE LFGHEKGAFT GAQSQRKGRF
EQADGGTLFL DEIGDMPADL QTRLLRVLAD GEFYRVGGHT PIKVDVRIIA ATHQDLEKGV
HAGKFREDLF HRLNVIRIHM PPLKDRKEDI PLLMKHFLEL AAKELNMEIK QLDKDTETYL
SQLDWPGNVR QLENTCRWLT VMSPGKIIHM EDLAPELLEE TRKSGEDSDW ENALRRWANQ
QLSR
//
