ID A0A0S8AU36_9BACT Unreviewed; 509 AA.
AC A0A0S8AU36;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KPK02220.1};
GN ORFNames=AMK71_02975 {ECO:0000313|EMBL:KPK02220.1};
OS Nitrospira bacterium SG8_35_4.
OC Bacteria; Nitrospirota; Nitrospiria.
OX NCBI_TaxID=1704025 {ECO:0000313|EMBL:KPK02220.1, ECO:0000313|Proteomes:UP000050997};
RN [1] {ECO:0000313|EMBL:KPK02220.1, ECO:0000313|Proteomes:UP000050997}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SG8_35_4 {ECO:0000313|EMBL:KPK02220.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPK02220.1}.
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DR EMBL; LJTM01000034; KPK02220.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S8AU36; -.
DR PATRIC; fig|1704025.3.peg.2220; -.
DR Proteomes; UP000050997; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF12; BLL1977 PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 1..126
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 149..389
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 509 AA; 59753 MW; 073F3D68C2E02CFA CRC64;
MKRVLFIETD LRNEKLGIMY LSAALKKRGH ETLLCRYEKE DFDMLIQSFS PDLVAFSLIT
GAHKRSLEIA RDIRKNYGIP TVAGGPHATF FSQDIPEDAV DFVVVGQGEK AIVDVVEGRA
RGRFLCYDFT DLNEIPFPDR SLFYRYEEFR DNPMKNVISM RDCPYSCSYC YNHSWKKMFR
GQNHFMQRRS VDGVIGEIKE MRDHYMLEKV LFIDDNFLIN DMWIGEFAIK YGREIGLPFL
CSFRVNLLDE EKLKMLRDAG LFMVNFALES ADPYVQSEIL HRDNIKNEHI IRAIELFRNY
DIKCRMQNMI GLPVEDSLQD ALGTLEFNKT YRVDDSWVSI FQPYPNTELA EYCFKNGFLE
ASMKDSIAES FFDESRLKID NKNKIKKLQK WWFFIVHYNL SDRLIDVILN IDINNAVSQS
LLDLRYKFSK KYLYEIENEN GSMLHYDWDD ICNRFKSAPN FKLWKHIIKK YELCGALSEI
LLDIDISDEI AKELANVQPI KEACLIDIS
//
