ID A0A0S8B6J0_9PROT Unreviewed; 756 AA.
AC A0A0S8B6J0;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN ORFNames=AMJ64_09340 {ECO:0000313|EMBL:KPK06365.1};
OS Betaproteobacteria bacterium SG8_39.
OC Bacteria; Pseudomonadota; Betaproteobacteria.
OX NCBI_TaxID=1703390 {ECO:0000313|EMBL:KPK06365.1, ECO:0000313|Proteomes:UP000051483};
RN [1] {ECO:0000313|EMBL:KPK06365.1, ECO:0000313|Proteomes:UP000051483}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SG8_39 {ECO:0000313|EMBL:KPK06365.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPK06365.1}.
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DR EMBL; LJTQ01000083; KPK06365.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S8B6J0; -.
DR STRING; 1703390.AMJ64_09340; -.
DR PATRIC; fig|1703390.3.peg.2729; -.
DR Proteomes; UP000051483; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 6..294
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 295..576
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 27..34
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 756 AA; 83144 MW; D373D78CE79805E6 CRC64;
MSLHLEHLNP EQLAAVTLPD EHALILAGAG SGKTRVLTTR VAWLVKSGRV SPGGVLAVTF
TNKAAREMYT RVSTMLPVNP RGMWIGTFHG LCNRMLRAHH RDAGLPQQFQ ILDTQDQLAA
VKRLLKSLNV DEDRFAPREV QYFFNARKEE GVRASNVEVY DETSRRFAEL YAAYDAQCQR
EGVVDFPELL LRSFELLARN ELLRAHYANR FRHILVDEFQ DTNRLQYRWL KLLAGASEGQ
GARPAPGGAA VFAVGDDDQS IYAFRGANVG NMADFERDFA VAHVVRLEQN YRSHGNILDA
ANALIANNRK RLGKNLWTAA GAGEPLRVYE GESDIDEARR IVDEAQSLAR EGTRFDHMAV
LYRSNAQSRV IEHALFSAGV AYRVYGGLRF FERAEVKHAL AYLRLVASPD DDNALLRVVN
FPPRGIGARS VEQLQDAARA QGSSLWRAAE SLGGRAAQSL AAFRALIEEM RAAADDLPLA
ELAEHVVAKS GLIEHYRHER EGADRIENLG ELVTAAAGFD VEEHASGLET GEGEADAPAI
DPLTAFLTHA ALEAGEHQAG EGQDALQLMT VHSAKGLEFD TVFLTGLEEG LFPHEQSALE
KDGLEEERRL AYVAITRARQ RLYLSFAQTR MLHGQTRYNL PSRFLEEIPE GVTKWLTPRF
AGQRAREAMR AASSGWSAPR KTAFGGAAAG RAHHAGFGAA AEVNGFRIGQ NVVHPKFGAG
VIVDAEGRGN DARVQVNFGR EGVKWLAVNV AKLQAA
//
