ID A0A0S8CBJ0_9CHLR Unreviewed; 233 AA.
AC A0A0S8CBJ0;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=precorrin-2 dehydrogenase {ECO:0000256|ARBA:ARBA00012400};
DE EC=1.3.1.76 {ECO:0000256|ARBA:ARBA00012400};
GN ORFNames=AMJ76_03115 {ECO:0000313|EMBL:KPK21253.1};
OS Dehalococcoidia bacterium SM23_28_1.
OC Bacteria; Chloroflexota; Dehalococcoidia.
OX NCBI_TaxID=1703395 {ECO:0000313|EMBL:KPK21253.1, ECO:0000313|Proteomes:UP000054497};
RN [1] {ECO:0000313|EMBL:KPK21253.1, ECO:0000313|Proteomes:UP000054497}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SM23_28_1 {ECO:0000313|EMBL:KPK21253.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin;
CC Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=1.3.1.76;
CC Evidence={ECO:0000256|ARBA:ARBA00001156};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme
CC biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
CC {ECO:0000256|ARBA:ARBA00005010}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPK21253.1}.
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DR EMBL; LJTZ01000069; KPK21253.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S8CBJ0; -.
DR UniPathway; UPA00262; UER00222.
DR Proteomes; UP000054497; Unassembled WGS sequence.
DR GO; GO:0004325; F:ferrochelatase activity; IEA:InterPro.
DR GO; GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 1.10.8.210; Sirohaem synthase, dimerisation domain; 1.
DR InterPro; IPR028161; Met8-like.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR037115; Sirohaem_synt_dimer_dom_sf.
DR InterPro; IPR019478; Sirohaem_synthase_dimer_dom.
DR InterPro; IPR006367; Sirohaem_synthase_N.
DR NCBIfam; TIGR01470; cysG_Nterm; 1.
DR PANTHER; PTHR35330; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR PANTHER; PTHR35330:SF1; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR Pfam; PF10414; CysG_dimeriser; 1.
DR Pfam; PF13241; NAD_binding_7; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF75615; Siroheme synthase middle domains-like; 1.
PE 4: Predicted;
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 153..206
FT /note="Sirohaem synthase dimerisation"
FT /evidence="ECO:0000259|Pfam:PF10414"
SQ SEQUENCE 233 AA; 26096 MW; 1C48B665C749CC60 CRC64;
MRSYPINLVG LENRRCIVVG GGEVAQRKAE ALLEAGAERV VVISPHLTHK LRALLKAKRI
EHRPRDYQQG DLEGAFVVIA ATDDPDVNRD VWQEAQEKRL LINVVDDPHR CNFFVPSVVR
RGDLTISICT GGQDPALSAR LRRQLEPRFG PEYAAFLEIA GALRDRVGKE LSARVRSRFW
NALADSEVLA LLRDGKWQEA ERLANDILAA HALKAKGRGR KRTPSLRDKQ SEA
//
