ID A0A0S8EE91_9BACT Unreviewed; 424 AA.
AC A0A0S8EE91;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KPK46234.1};
GN ORFNames=AMK74_00850 {ECO:0000313|EMBL:KPK46234.1};
OS Nitrospira bacterium SM23_35.
OC Bacteria; Nitrospirota; Nitrospiria.
OX NCBI_TaxID=1704026 {ECO:0000313|EMBL:KPK46234.1, ECO:0000313|Proteomes:UP000051169};
RN [1] {ECO:0000313|EMBL:KPK46234.1, ECO:0000313|Proteomes:UP000051169}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SM23_35 {ECO:0000313|EMBL:KPK46234.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPK46234.1}.
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DR EMBL; LJUG01000006; KPK46234.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S8EE91; -.
DR Proteomes; UP000051169; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485}.
FT DOMAIN 3..142
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 180..404
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 424 AA; 48684 MW; 65915ACE3A5FA70A CRC64;
MTKKQIILIS YNWANHFSLA LGYLKAYSLK DPFIRQHTNI EILDFDTAGL NVQQVIYYLS
QSKPDIIGFS CYCWNIEKVL DTARILKTLY PDMKIVLGGP EVGPIGPKYL GEHPFLDVVI
KGEGEITFAE LLKYCLGECR LEDIAGISYR FNGRVIENPD RPPIENLSEI PSPYLEGILI
PRDRVTYIET YRGCIFKCHY CFEGKNLPKL RFFPDDRVQR EIELVLSHPE VKSFHFVDTV
FNFRKERLAK MAGIIASANR YGAELRTVEI IAEFVDEETV EHFKKAHVRS IETGPQTVNE
NTLKNVNRFY KEEQFRNGVR LLEDNGIEVT TDLIIGLPGD TFFKFMNSVQ TIIDMKPSNL
VFSILHVLPG TVLYEKSMEF GLKYDDKAPH LVLSAPDFPY EDIDKAVIMA FSLDKEYNIK
PLPP
//
