ID A0A0S8FE59_9BACT Unreviewed; 729 AA.
AC A0A0S8FE59;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=AMK73_08890 {ECO:0000313|EMBL:KPK59011.1};
OS Planctomycetes bacterium SM23_32.
OC Bacteria; Planctomycetota.
OX NCBI_TaxID=1704029 {ECO:0000313|EMBL:KPK59011.1, ECO:0000313|Proteomes:UP000052164};
RN [1] {ECO:0000313|EMBL:KPK59011.1, ECO:0000313|Proteomes:UP000052164}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SM23_32 {ECO:0000313|EMBL:KPK59011.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPK59011.1}.
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DR EMBL; LJUE01000187; KPK59011.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S8FE59; -.
DR PATRIC; fig|1704029.3.peg.1791; -.
DR Proteomes; UP000052164; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 12..287
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 288..558
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 33..40
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 729 AA; 82097 MW; 0BF2BC275F5A3AE5 CRC64;
MPKRSRLDQI TADLTDAQRE AVCYGEGPLL VLAGAGSGKT RVITRRIAYL LGCGVAPDRI
IAVTFTNKAA DEMRRRVEAL VGKDVYVSTF HSFCARLLRR EIGRLGRDAS FTIYDRADSL
RVVRRVVREM ELDPATYSPR ALLDFIGLRK DRVEGPRECR QRALGIEEQT WADVYQRYEE
QLASNNALDF DDLLLRTLEV FRESPGALQH YQDAYLHVLV DEYQDTNLPQ HLIARALQGK
HRNITAVGDP DQTIYTWRGA RLENLMEFEQ DFPGAHVVTL KRNYRSSANI LRAASACISH
NVFRRPKELH TERDEGEPIV VAQFADSFAE GRWVAEMVQE LMQDRVAPRE IAVFYRTKNQ
SLPLEDAFAS LDLPHQVVDS VGFFERRQVK DLRSYLQLLI NPRDDVACLR VINTPPRGIG
ARTVEKLQAA AVQRGTSLLE AMRDAEQIEA LGPRAARAAR GFYELHARLS ALRARSAKKL
LSRAIELTGY LERQPADERE DAEELLDMFM DYAGQYDKRS PEGGLMGFLE QSALVSDVDG
WNDQAGAVPF MTLHSAKGLE FDAVFIVGVE RGMLPHQRAM QEHVHDTDRA ALEEERRLFY
VGMTRARHRL FISHARKRTL RGRQEQAEPS PFLEELPAEG VERLGASGRA AASFGREMDY
VLKQKKLSLR ILDGGANRLA AGARVSHPVY GEGTILETMR MGSRHVVRVD FAAHGPMVLV
LAAADVGRA
//
