ID A0A0S8FML3_9GAMM Unreviewed; 710 AA.
AC A0A0S8FML3;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE SubName: Full=3-hydroxyacyl-CoA dehydrogenase {ECO:0000313|EMBL:KPK61003.1};
GN ORFNames=AMJ59_03300 {ECO:0000313|EMBL:KPK61003.1};
OS Gammaproteobacteria bacterium SG8_31.
OC Bacteria; Pseudomonadota; Gammaproteobacteria.
OX NCBI_TaxID=1703405 {ECO:0000313|EMBL:KPK61003.1, ECO:0000313|Proteomes:UP000051321};
RN [1] {ECO:0000313|EMBL:KPK61003.1, ECO:0000313|Proteomes:UP000051321}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SG8_31 {ECO:0000313|EMBL:KPK61003.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
CC -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA
CC dehydrogenase family. {ECO:0000256|ARBA:ARBA00007005}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPK61003.1}.
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DR EMBL; LJTI01000003; KPK61003.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S8FML3; -.
DR PATRIC; fig|1703405.3.peg.644; -.
DR UniPathway; UPA00659; -.
DR Proteomes; UP000051321; Unassembled WGS sequence.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.1040.50; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43612; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA; 1.
DR PANTHER; PTHR43612:SF3; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA, MITOCHONDRIAL; 1.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 320..498
FT /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT /evidence="ECO:0000259|Pfam:PF02737"
FT DOMAIN 501..598
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
SQ SEQUENCE 710 AA; 77784 MW; DE8D6E891FD93649 CRC64;
MSETIKYQKD EGGIVTLTID IADRSMNVLT PRFMRDLDAA VDRIVADDSV RGAIITSAKT
SFIAGADLKE LVGVFGREMD EHQIYEFAGR YSQLYRKLET CGKPFVAAIN GTALGGGLEL
CLACHHRVVL DNPKAAIGLP EVKVGLLPGA GGTQRLPRLI GIRESLPLML EGRHLKPPKA
VELGICNELA TDVDDMMERA RRWLADVGDP VAPWDKKGFK VPGGSGGMHP AVVQTFMAGN
AMVAEKTQHR YPAPISIMSC VYEGTQIPID KGLKVELQYF TKLLAGPVAR NMIRTLFIDK
GAADKLARRP ADVPKSHVKK LGVLGAGMMG AGIAYVSAYA GQEVVLLDRA LEDAEKGKGY
SRALLEKRVS RGRMTEDQAA AVLERIKPTT DYGDLAGCDL VIEAVFEDRA IKADVTEKTE
AVIPETSIFA SNTSTLPITG LAQASRRPEQ FIGIHFFSPV DKMPLVEIIL GEKTGQEAIA
RALDYVQQIR KTPILVNDSR GFYTSRVFST YVKEGMELLA EGVKPALIEN AAKMAGMPVG
PLAVHDEVTI DLSYKILMQT KKDLGDDFLP TQADEVVRHF QEDLKRRGKR FGAGFYEYPE
GARKYLWPGL AEEYPPAPEQ PDLEYIQRRL LYIQCVEAAR CMEEGVVPEP ADADVGSILG
WGFPPWTGGT LSLIDTVGVR DFVDQCDALA EQHGPRFAPP DWLRQRSSAR
//
