ID A0A0S8G189_9BACT Unreviewed; 425 AA.
AC A0A0S8G189;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369, ECO:0000256|RuleBase:RU003370};
GN Name=gltA {ECO:0000313|EMBL:KPK66606.1};
GN ORFNames=AMS21_01375 {ECO:0000313|EMBL:KPK66606.1};
OS Gemmatimonas sp. SG8_38_2.
OC Bacteria; Gemmatimonadota; Gemmatimonadetes; Gemmatimonadales;
OC Gemmatimonadaceae; Gemmatimonas.
OX NCBI_TaxID=1703358 {ECO:0000313|EMBL:KPK66606.1, ECO:0000313|Proteomes:UP000051763};
RN [1] {ECO:0000313|EMBL:KPK66606.1, ECO:0000313|Proteomes:UP000051763}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SG8_38_2 {ECO:0000313|EMBL:KPK66606.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000308,
CC ECO:0000256|RuleBase:RU003370};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2. {ECO:0000256|ARBA:ARBA00004751,
CC ECO:0000256|RuleBase:RU003370}.
CC -!- SIMILARITY: Belongs to the citrate synthase family.
CC {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC ECO:0000256|RuleBase:RU003406}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPK66606.1}.
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DR EMBL; LJTP01000003; KPK66606.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S8G189; -.
DR PATRIC; fig|1703358.3.peg.1788; -.
DR UniPathway; UPA00223; UER00717.
DR Proteomes; UP000051763; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd06114; EcCS_like; 1.
DR Gene3D; 2.20.28.60; -; 1.
DR Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR InterPro; IPR010953; Citrate_synthase_typ-I.
DR NCBIfam; TIGR01798; cit_synth_I; 1.
DR PANTHER; PTHR42871; CITRATE SYNTHASE; 1.
DR PANTHER; PTHR42871:SF1; CITRATE SYNTHASE; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; Citrate synthase; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:KPK66606.1};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW ECO:0000256|RuleBase:RU003370}.
FT ACT_SITE 303
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT ACT_SITE 360
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ SEQUENCE 425 AA; 48474 MW; EA6D93C76FC7DF8B CRC64;
MTKKAKLEYD DKSYEMAIVE GTYAERAVDI RGLRKDTGLI TLDSGYMNTG STESRITYIN
GEEGILLYRG YPIEQLAEAS TFVETSYLLI YGELPTKTQL KEFSTLLTYH SMLHEDMRHF
FDGFPSNAHP MAILSSMVSA LSAYYPDSLE PDTFNKQHIA RLLSKLRTIA AFSYQKKQGK
PVAYPRNELS YCANFMHMMF SHPAEPEYVP DPEIVKVLNI LLILHADHEQ NCSTSTVRLV
GSSRANLYAA ISAGISALWG PLHGGANQAV IDMLHDIIAE GGDCKAFMEH VKNRERRLMG
FGHRVYKNFD PRATILKKYA DKLLKHLGMD DPLFDIARQL EEVALSDDFF IERKLYPNVD
FYSGILYRAI GIPTEMFTVM FALGRIPGWI AHWKEMMEDP DLRIGRPRQV YMGQTKRDYV
KIEAR
//
