ID A0A0S8L5N9_9PROT Unreviewed; 1142 AA.
AC A0A0S8L5N9;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN ORFNames=AMJ72_01100 {ECO:0000313|EMBL:KPL28845.1};
OS Acidithiobacillales bacterium SM1_46.
OC Bacteria; Pseudomonadota; Acidithiobacillia; Acidithiobacillales.
OX NCBI_TaxID=1703384 {ECO:0000313|EMBL:KPL28845.1, ECO:0000313|Proteomes:UP000052054};
RN [1] {ECO:0000313|EMBL:KPL28845.1, ECO:0000313|Proteomes:UP000052054}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SM1_46 {ECO:0000313|EMBL:KPL28845.1};
RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6;
RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.;
RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur cycling
RT among widespread estuary sediment bacteria.";
RL Microbiome 3:14-14(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPL28845.1}.
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DR EMBL; LJVB01000005; KPL28845.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0S8L5N9; -.
DR PATRIC; fig|1703384.3.peg.2095; -.
DR Proteomes; UP000052054; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 5..502
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 514..780
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1142 AA; 124662 MW; 4E11E245C5513F83 CRC64;
MSTERLADTN AREAALDAAR SFIVQAPAGS GKTELLTNRY LVLLGRVDHP EEVLAITFTR
KAAAEMRSRI IEALAAAATG AELEAPHKQR MVALARAALA RDAERGWNLL AQPQRLRIST
IDALCTGLTR QMPVLSRTGG AVRITEQGET LYREAARELL ALLEADHDWT PAVERLLAHL
DNDLPQVESL LASMLARRDQ WLRHLATPDD PRRHRAVLET ALTHAIEDEL ARAAAALPTA
VAAALLGVLR AATANLRAAG SDSALIAWEE CVALLAPRVG NLAAWQALAG LLTTSTDGGA
WRARVTRSEG FPPPSSAKGK EKARLAAAKA EFEALIAQLA EAPELLGRLR ALVALPGPHY
ADAQWQLIEA LGALLPLAVA QLRLVFARHG EVDFTEVSWS ALEALGRPEA PSDLALLLDY
RIAHLLVDEF QDTSLSQYEL LERLTAGWQP DDGRTLFLVG DPMQSIYRFR QAEVGLYLRA
RAQGLGSVRL TPLALEVNFR SQAGIVDWVN RAFATAFPAE ENIALGAVTH AHSVAANGAL
HGQAVTVHPF FDKDHVAEAE QVAALVQAAL ADPAQRTIAI LVRSRSHLEE IVPRLRAAGI
PFRAIDIEPL GGRPVVQDLL VLTRALVHRA DRLSWLAVLR APWCGLQLAD LDALCARDRD
TAVWTLMQDD AHVARLSDDG QARLVRVRDV LAAALQRERR APLRQLVEGT WLALGGSACA
PGAAALADAR AFFDLLDTLE QGGDLADLAL LDQQVGELHA APDVDAGERL QVMTVHKAKG
LEFDTVIVPG LGRQPRAADA PLLRWHEMAR AGATVAELLL APMRARGEKQ DPVYDFLGGY
ERIQADFEDT RLLYVASTRA IRHLHLLGAV AIEETEEGGR ELREPPARSL LARLWPAAAG
EFERALARGA HRHVTATHEA ATDNVAIRRL TSAWRTPAPP AQVAWRKRAE PVRSDRTVVA
FDWASETIRS VGSLVHRWLE ILAREGIAAW SEERVRALTP VFARNLAELG VPADRIGAAI
ERVSEALTHA LTDPRGRWIL DSAHGEARTE YALTGVDQGE LVSVVLDRTF VDGDGVRWII
DYKTGTHEGG DPDVFLDREQ QRYAAQLARY ARLMYLLDAR PIRLGLYFPL LGAWREWPAV
GD
//
