ID A0A0T5ZKD1_9CHLR Unreviewed; 494 AA.
AC A0A0T5ZKD1;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Tyrosine phenol-lyase {ECO:0000256|ARBA:ARBA00016166, ECO:0000256|HAMAP-Rule:MF_00543};
DE EC=4.1.99.2 {ECO:0000256|ARBA:ARBA00013154, ECO:0000256|HAMAP-Rule:MF_00543};
DE AltName: Full=Beta-tyrosinase {ECO:0000256|ARBA:ARBA00033449, ECO:0000256|HAMAP-Rule:MF_00543};
GN Name=tpl {ECO:0000256|HAMAP-Rule:MF_00543};
GN ORFNames=XU10_C0013G0019 {ECO:0000313|EMBL:KRT63251.1};
OS Chloroflexi bacterium CSP1-4.
OC Bacteria; Chloroflexota.
OX NCBI_TaxID=1640513 {ECO:0000313|EMBL:KRT63251.1, ECO:0000313|Proteomes:UP000051896};
RN [1] {ECO:0000313|EMBL:KRT63251.1, ECO:0000313|Proteomes:UP000051896}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CSP1-4 {ECO:0000313|EMBL:KRT63251.1};
RA Hug L.A., Thomas B.C., Sharon I., Brown C.T., Sharma R., Hettich R.L.,
RA Wilkins M.J., Williams K.H., Singh A., Banfield J.F.;
RT "Critical biogeochemical functions in the subsurface are associated with
RT bacteria from new phyla and little studied lineages.";
RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-tyrosine = NH4(+) + phenol + pyruvate;
CC Xref=Rhea:RHEA:21704, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15882, ChEBI:CHEBI:28938, ChEBI:CHEBI:58315; EC=4.1.99.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001821, ECO:0000256|HAMAP-
CC Rule:MF_00543};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000256|ARBA:ARBA00001933,
CC ECO:0000256|HAMAP-Rule:MF_00543, ECO:0000256|PIRSR:PIRSR611166-50};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881,
CC ECO:0000256|HAMAP-Rule:MF_00543}.
CC -!- SIMILARITY: Belongs to the beta-eliminating lyase family.
CC {ECO:0000256|ARBA:ARBA00009721, ECO:0000256|HAMAP-Rule:MF_00543}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRT63251.1}.
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DR EMBL; LDXM01000013; KRT63251.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0T5ZKD1; -.
DR STRING; 1640513.XU10_C0013G0019; -.
DR PATRIC; fig|1640513.3.peg.1321; -.
DR Proteomes; UP000051896; Unassembled WGS sequence.
DR GO; GO:0050371; F:tyrosine phenol-lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006570; P:tyrosine metabolic process; IEA:InterPro.
DR CDD; cd00617; Tnase_like; 1.
DR Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR HAMAP; MF_00543; Tyr_phenol_lyase; 1.
DR InterPro; IPR001597; ArAA_b-elim_lyase/Thr_aldolase.
DR InterPro; IPR011166; Beta-eliminating_lyase.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR InterPro; IPR013441; Tyr_phenol_ly.
DR NCBIfam; TIGR02618; tyr_phenol_ly; 1.
DR PANTHER; PTHR32325; BETA-ELIMINATING LYASE-LIKE PROTEIN-RELATED; 1.
DR PANTHER; PTHR32325:SF4; TRYPTOPHANASE; 1.
DR Pfam; PF01212; Beta_elim_lyase; 1.
DR PIRSF; PIRSF001386; Trpase; 1.
DR SUPFAM; SSF53383; PLP-dependent transferases; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|HAMAP-Rule:MF_00543, ECO:0000313|EMBL:KRT63251.1};
KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898, ECO:0000256|HAMAP-
KW Rule:MF_00543}.
FT DOMAIN 62..438
FT /note="Aromatic amino acid beta-eliminating lyase/threonine
FT aldolase"
FT /evidence="ECO:0000259|Pfam:PF01212"
FT MOD_RES 273
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00543,
FT ECO:0000256|PIRSR:PIRSR611166-50"
SQ SEQUENCE 494 AA; 54772 MW; 004DEF14F0ADD0EC CRC64;
MPDDTFVPHT MGQQFGRRSW AEPWKIKMVE PIRQLDRAGR ERAIREAGYN TFLLRSDDVY
IDLLTDSGTN AMSDRQWAAI MLGDEAYAGS RNFYHLEGAI QRRFGYRYVV PTHQGRGAEH
LISRTAIKPG QWVPGNMYFT TTRLHQELAG GTFVDVIIDE AHDPTSLHPF KGDMDLAKLE
ALIARVGPEQ IAYVSLAATV NMAGGQPVSM ANARAVSELC QRHGIRLFLD ATRVVENAFF
IEEREPGFAG RSVASIVHEF CSHADGAWMS AKKDGLVNIG GWLAVNDEEL FEELRNLVVV
FEGLHTYGGL AGRDMEALAV GIEESLEDAY IRARVGQVRY LGELLTDWGV AIVQPVGGHA
VFLDARRFYP HLPQDAFPAQ TLAAELYLDS GVRAMERGIA SAGRDPKTGD HHRPKLELTR
LTIPRRVYTQ AHMDVVAESV KAVWDARESA TGLRMVYEPK YLRFFQAKFE PLAAGETATS
EAGGSEAAYS SSRG
//
