ID A0A0T6A8W7_9BACT Unreviewed; 441 AA.
AC A0A0T6A8W7;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=L-cysteine S-thiosulfotransferase subunit SoxA {ECO:0000256|ARBA:ARBA00019364};
DE EC=2.8.5.2 {ECO:0000256|ARBA:ARBA00012408};
GN ORFNames=XU13_C0051G0005 {ECO:0000313|EMBL:KRT71358.1};
OS Candidatus Rokubacteria bacterium CSP1-6.
OC Bacteria; Candidatus Rokubacteria.
OX NCBI_TaxID=1640509 {ECO:0000313|EMBL:KRT71358.1, ECO:0000313|Proteomes:UP000051916};
RN [1] {ECO:0000313|EMBL:KRT71358.1, ECO:0000313|Proteomes:UP000051916}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CSP1-6 {ECO:0000313|EMBL:KRT71358.1};
RA Hug L.A., Thomas B.C., Sharon I., Brown C.T., Sharma R., Hettich R.L.,
RA Wilkins M.J., Williams K.H., Singh A., Banfield J.F.;
RT "Critical biogeochemical functions in the subsurface are associated with
RT bacteria from new phyla and little studied lineages.";
RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 Fe(III)-[cytochrome c] + L-cysteinyl-[SoxY protein] +
CC thiosulfate = 2 Fe(II)-[cytochrome c] + 2 H(+) + S-sulfosulfanyl-L-
CC cysteinyl-[SoxY protein]; Xref=Rhea:RHEA:56720, Rhea:RHEA-COMP:10350,
CC Rhea:RHEA-COMP:14328, Rhea:RHEA-COMP:14399, Rhea:RHEA-COMP:14691,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034,
CC ChEBI:CHEBI:29950, ChEBI:CHEBI:33542, ChEBI:CHEBI:139321; EC=2.8.5.2;
CC Evidence={ECO:0000256|ARBA:ARBA00029285};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 Fe(III)-[cytochrome c] + S-sulfanyl-L-cysteinyl-[SoxY
CC protein] + thiosulfate = 2 Fe(II)-[cytochrome c] + 2 H(+) + S-(2-
CC sulfodisulfanyl)-L-cysteinyl-[SoxY protein]; Xref=Rhea:RHEA:51224,
CC Rhea:RHEA-COMP:10350, Rhea:RHEA-COMP:14399, Rhea:RHEA-COMP:14689,
CC Rhea:RHEA-COMP:14690, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033,
CC ChEBI:CHEBI:29034, ChEBI:CHEBI:33542, ChEBI:CHEBI:61963,
CC ChEBI:CHEBI:140664; EC=2.8.5.2;
CC Evidence={ECO:0000256|ARBA:ARBA00029308};
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000256|ARBA:ARBA00004418}.
CC -!- SIMILARITY: Belongs to the SoxA family.
CC {ECO:0000256|ARBA:ARBA00025746}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRT71358.1}.
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DR EMBL; LDXP01000051; KRT71358.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0T6A8W7; -.
DR STRING; 1640509.XU13_C0051G0005; -.
DR Proteomes; UP000051916; Unassembled WGS sequence.
DR GO; GO:0070069; C:cytochrome complex; IEA:InterPro.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016669; F:oxidoreductase activity, acting on a sulfur group of donors, cytochrome as acceptor; IEA:InterPro.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0019417; P:sulfur oxidation; IEA:InterPro.
DR Gene3D; 1.10.760.10; Cytochrome c-like domain; 3.
DR InterPro; IPR009056; Cyt_c-like_dom.
DR InterPro; IPR036909; Cyt_c-like_dom_sf.
DR InterPro; IPR025710; SoxA.
DR InterPro; IPR030999; Thiosulf_SoxX.
DR NCBIfam; TIGR04484; thiosulf_SoxA; 1.
DR NCBIfam; TIGR04485; thiosulf_SoxX; 1.
DR Pfam; PF13442; Cytochrome_CBB3; 1.
DR Pfam; PF21342; SoxA-TsdA_cyt-c; 1.
DR SUPFAM; SSF46626; Cytochrome c; 3.
DR PROSITE; PS51007; CYTC; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Electron transport {ECO:0000256|ARBA:ARBA00022982};
KW Heme {ECO:0000256|ARBA:ARBA00022617, ECO:0000256|PROSITE-ProRule:PRU00433};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PROSITE-ProRule:PRU00433};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|PROSITE-
KW ProRule:PRU00433}; Periplasm {ECO:0000256|ARBA:ARBA00022764};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..441
FT /note="L-cysteine S-thiosulfotransferase subunit SoxA"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5006667677"
FT DOMAIN 102..194
FT /note="Cytochrome c"
FT /evidence="ECO:0000259|PROSITE:PS51007"
FT REGION 195..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 197..211
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 441 AA; 48213 MW; 4E32F51F02EEC28F CRC64;
MLRPGTVFGG GIVFAALLSA ACAQTDKAST AGLPPAPWIS RAIPEGKGEV KTQPDGKKMQ
VRYKRAPFAG VPQDWSDYRT YAYDDTRPAP PVSKAPMTQV KGDPARGRRL FLSRAQGPCT
GCHLIPGADV WPAGNIGPDL STYGDRNLPD EFIFTQLYDS RNFFPHTTMP PWGTTGVFKP
EELVHIVAFL KTLKGPVPPE KDPDRNPDTR AKPVGFGDNL DPTNNPAVLL AEGAMAVWSK
KGPAGKACAD CHAGGPEKSM KEAATRYPKY VKAYQRVMSI EDFLTVHAPE TTGLQMLSQS
ADNLNMTMLV KMQSNGMPLK VDVTSPEAKG AYERGRASFF KRVGQRNHAC ADCHTKGAGR
GSDKFLGGRL LADVEVGLTK HFPLWRTSQG QVWDMRKRMQ WCMTPLGMNM LPADAVEYAE
LELYLTAFDN GKEMSVPGIR H
//
