ID A0A0T6AJ19_9DELT Unreviewed; 1217 AA.
AC A0A0T6AJ19;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 08-NOV-2023, entry version 31.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN ORFNames=XU12_C0001G0177 {ECO:0000313|EMBL:KRT74986.1};
OS Deltaproteobacteria bacterium CSP1-8.
OC Bacteria; Deltaproteobacteria.
OX NCBI_TaxID=1640514 {ECO:0000313|EMBL:KRT74986.1, ECO:0000313|Proteomes:UP000051991};
RN [1] {ECO:0000313|EMBL:KRT74986.1, ECO:0000313|Proteomes:UP000051991}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CSP1-8 {ECO:0000313|EMBL:KRT74986.1};
RA Hug L.A., Thomas B.C., Sharon I., Brown C.T., Sharma R., Hettich R.L.,
RA Wilkins M.J., Williams K.H., Singh A., Banfield J.F.;
RT "Critical biogeochemical functions in the subsurface are associated with
RT bacteria from new phyla and little studied lineages.";
RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRT74986.1}.
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DR EMBL; LDXO01000001; KRT74986.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0T6AJ19; -.
DR STRING; 1640514.XU12_C0001G0177; -.
DR PATRIC; fig|1640514.3.peg.190; -.
DR Proteomes; UP000051991; Unassembled WGS sequence.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00932; MOLYBDOPTERIN_PROK_3; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000313|EMBL:KRT74986.1}.
FT DOMAIN 26..90
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 1217 AA; 137536 MW; 9FD0E88F25AE6C85 CRC64;
MSWIRDIADP RNRRWEEFYR NRWQHDKVVR STHGVNCTGG CSWMVHVKDG IITWELQATD
YPRLAGDLPP YEPRGCQRGI STSWYVYSPI RVKYPYVRGA LMDLWRKARA KHDDPVAAWT
SLMEDGESRR AYQRARGKGG FRRASWEEVL EIIAASAIHT VKRHGADRII GFSPIPAMSM
LSYAGGSRFL QLLGGVNLSF YDWYSDLPPA SPEVWGEQTD VAESADWYNS KYIVVMGANL
GMTRTPDVHF AAEARHNGSK LVVLSPDFSM VSKYADWWIP ANAGQDGAFW MAVNHVILRE
FFAEKRTPYF EDYLKRYSDA PFLVVVDSRG EAGSGGPVAD SKRLLRARCL NRYRDVENAD
WKFLVWDDTT GGPRMPLGSV GFRWQEEKGK WNLGMKDGLD GKEISPRLSM LGVHDDVLPL
EVADFSENRT FARGVPVKYV ETDGGKVAVA TVFDLIMAQF GVSRGLPGEY PERYEDEAIP
YTPAWQEKFT GIEAKTVLQF AREFASTAEK TEGRCSIIIG AGVNHWYHNN LMYRAGIVGL
ILCGCVGKNG GGLNHYVGQE KLAPLAPWST LAFALDWGKP PRLQNAPSFH YVHSCQWRYE
RGFDEYNSPL TAGAGNGDGR FTRGHTMDLQ AKAVRMGWLP FYPQFNENPL DIVREAEEAG
AKADGEIVRR VTERLKEGSL RFAVEDPDAP ENWPRLWIIW RGNALMSSAK GHEYFLRHYL
GTHHNMVSEE IAEGTSAEIS WREPAPEGKL DLVVDINFRM DTSALYSDIV LPTATWYEKN
DLNSTDLHSY IHPLSAAVPP CWESKSDWDI FKAIAEKVSN LAPAHFPGKV RDIVAVPLLH
DTPAELAQPV VKDWKAGECD LVPGKTMPNL VVVERDYANL YRRFLSFGPL ARKDGIGVHG
LSWPIEDLYD ELAQRRPTAE WNGRAYPSVE EAVDAANLIL HLAPETNGES AYRAFKAEEK
KVGLPLADLA EGVREVRYTF EELARQPRRL LTTPCWTGIT NKGRAYSAYC LNVERLVPWR
TLTGRQHLYL DHEGYIAFGE HLPTYKPIPD PRSMGDISRS RPEGKSVLLN YLTPHGKWHI
HSTFFDNERM LTLSRGIEPL WMNDRDAADA GIADNDWVEL YNDHGVVVTR AAVSARLPRK
MCMLYHSPER TISVPRSPLR GNRRAGGHNS LTRTRLKPVL MMGGYAQFTY GFNYWGPTGV
NRDTFVYVRK LPGAPAW
//
