ID A0A0T9Y5I4_SALET Unreviewed; 453 AA.
AC A0A0T9Y5I4;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 24-JAN-2024, entry version 47.
DE RecName: Full=PTS system N-acetylmuramic acid-specific EIIBC component {ECO:0000256|ARBA:ARBA00040399};
DE EC=2.7.1.192 {ECO:0000256|ARBA:ARBA00039021};
DE AltName: Full=EIIBC-MurNAc {ECO:0000256|ARBA:ARBA00043021};
GN ORFNames=B7N01_14300 {ECO:0000313|EMBL:EDG4995593.1}, B7N80_12120
GN {ECO:0000313|EMBL:EDG5289122.1}, E0916_16675
GN {ECO:0000313|EMBL:ECF1447580.1}, EJV93_08215
GN {ECO:0000313|EMBL:ECA2721189.1}, ERS008198_04580
GN {ECO:0000313|EMBL:CNV18200.1}, G0B02_19020
GN {ECO:0000313|EMBL:HAC6381169.1}, G0D18_16330
GN {ECO:0000313|EMBL:HAC6682187.1}, G2206_20860
GN {ECO:0000313|EMBL:HAE0437192.1}, NCTC5754_01341
GN {ECO:0000313|EMBL:SUE46501.1};
OS Salmonella enterica subsp. enterica serovar Bovismorbificans.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=58097 {ECO:0000313|EMBL:SUE46501.1, ECO:0000313|Proteomes:UP000254190};
RN [1] {ECO:0000313|EMBL:CNV18200.1, ECO:0000313|Proteomes:UP000041314}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A1104 {ECO:0000313|EMBL:CNV18200.1,
RC ECO:0000313|Proteomes:UP000041314};
RG Pathogen Informatics;
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:HAC6381169.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=BCW_3301 {ECO:0000313|EMBL:HAC6381169.1}, M123
RC {ECO:0000313|EMBL:HAC6682187.1}, and MS140073
RC {ECO:0000313|EMBL:HAE0437192.1};
RX PubMed=30286803;
RA Souvorov A., Agarwala R., Lipman D.J.;
RT "SKESA: strategic k-mer extension for scrupulous assemblies.";
RL Genome Biol. 19:153-153(2018).
RN [3] {ECO:0000313|EMBL:SUE46501.1, ECO:0000313|Proteomes:UP000254190}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC5754 {ECO:0000313|EMBL:SUE46501.1,
RC ECO:0000313|Proteomes:UP000254190};
RG Pathogen Informatics;
RA Doyle S.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:HAE0437192.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=BCW_3301 {ECO:0000313|EMBL:HAC6381169.1}, M123
RC {ECO:0000313|EMBL:HAC6682187.1}, and MS140073
RC {ECO:0000313|EMBL:HAE0437192.1};
RG NCBI Pathogen Detection Project;
RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:ECF1447580.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=136768 {ECO:0000313|EMBL:EDG4995593.1}, 138330
RC {ECO:0000313|EMBL:EDG5289122.1}, 598023
RC {ECO:0000313|EMBL:ECA2721189.1}, and 692616
RC {ECO:0000313|EMBL:ECF1447580.1};
RA Ashton P.M., Dallman T., Nair S., De Pinna E., Peters T., Grant K.;
RL Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N(pros)-phospho-L-histidyl-[protein] + N-acetyl-beta-D-
CC muramate(out) = L-histidyl-[protein] + N-acetyl-beta-D-muramate 6-
CC phosphate(in); Xref=Rhea:RHEA:33399, Rhea:RHEA-COMP:9745, Rhea:RHEA-
CC COMP:9746, ChEBI:CHEBI:29979, ChEBI:CHEBI:58721, ChEBI:CHEBI:64837,
CC ChEBI:CHEBI:64848; EC=2.7.1.192;
CC Evidence={ECO:0000256|ARBA:ARBA00036529};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; CQPA01000065; CNV18200.1; -; Genomic_DNA.
DR EMBL; AAHTVM010000004; ECA2721189.1; -; Genomic_DNA.
DR EMBL; AAIKGE010000011; ECF1447580.1; -; Genomic_DNA.
DR EMBL; AAMEJW010000014; EDG4995593.1; -; Genomic_DNA.
DR EMBL; AAMEML010000011; EDG5289122.1; -; Genomic_DNA.
DR EMBL; DAAMEZ010000015; HAC6381169.1; -; Genomic_DNA.
DR EMBL; DAAMHM010000013; HAC6682187.1; -; Genomic_DNA.
DR EMBL; DAAQQK010000031; HAE0437192.1; -; Genomic_DNA.
DR EMBL; UGVQ01000002; SUE46501.1; -; Genomic_DNA.
DR RefSeq; WP_000361660.1; NZ_WFIN01000006.1.
DR AlphaFoldDB; A0A0T9Y5I4; -.
DR Proteomes; UP000041314; Unassembled WGS sequence.
DR Proteomes; UP000254190; Unassembled WGS sequence.
DR Proteomes; UP000839929; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00212; PTS_IIB_glc; 1.
DR Gene3D; 3.30.1360.60; Glucose permease domain IIB; 1.
DR InterPro; IPR036878; Glu_permease_IIB.
DR InterPro; IPR018113; PTrfase_EIIB_Cys.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR013013; PTS_EIIC_1.
DR InterPro; IPR001996; PTS_IIB_1.
DR PANTHER; PTHR30175; PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN; 1.
DR PANTHER; PTHR30175:SF3; PTS SYSTEM N-ACETYLMURAMIC ACID-SPECIFIC EIIBC COMPONENT; 1.
DR Pfam; PF00367; PTS_EIIB; 1.
DR Pfam; PF02378; PTS_EIIC; 1.
DR SUPFAM; SSF55604; Glucose permease domain IIB; 1.
DR PROSITE; PS51098; PTS_EIIB_TYPE_1; 1.
DR PROSITE; PS01035; PTS_EIIB_TYPE_1_CYS; 1.
DR PROSITE; PS51103; PTS_EIIC_TYPE_1; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597,
KW ECO:0000313|EMBL:ECA2721189.1};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 115..136
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 214..231
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 243..264
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 325..345
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 357..378
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 425..447
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 5..88
FT /note="PTS EIIB type-1"
FT /evidence="ECO:0000259|PROSITE:PS51098"
FT DOMAIN 113..453
FT /note="PTS EIIC type-1"
FT /evidence="ECO:0000259|PROSITE:PS51103"
FT ACT_SITE 27
FT /note="Phosphocysteine intermediate; for EIIB activity"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00421"
SQ SEQUENCE 453 AA; 47219 MW; 37A8DEB72020D4F8 CRC64;
MDKTAALASD ILRGIGGEQN ILRLENCMTR VRVEVQDDSQ LDIPRLKALP GVSGYVKQGE
QHQLIVGPGK AAQVVDAMRV QIAAGGVKPD DAMARTKSEA KAKYKAPMSD ALRKLANVFI
PLIPAFIASG LITGIINILK RPDIVGDVAV HYPNLLGLMG IFGSAVFAIM NILVGVNTAK
VFGGSQALGG VMAGILSSPQ LAQITLFGEA LQPGRGGVIA VLLVVALMCW IERQFRKLLP
GSLELILNPL LTTVITGAVA IVALQPLGGW ISDAIAHGAS WAIDRGGFLV GAVLAGTFLP
LVLTGLHQGL VPIHVELVQA HGYNALFPIL AMAGVGQIGA AIAVLMKTRN ARLKKVIKGA
LPVGLLGIGE PLIFGVTLPL GKPFIGACLG GAVGGALISY WKVATVITFG ISGLPLALTI
VAGKVLFYLL GYLIAVIAGF IFTWLLGFND PEE
//
