ID A0A0U2WYV9_9ENTE Unreviewed; 554 AA.
AC A0A0U2WYV9;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000256|HAMAP-Rule:MF_01543};
GN ORFNames=ATZ35_08660 {ECO:0000313|EMBL:ALS37226.1};
OS Enterococcus rotai.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=118060 {ECO:0000313|EMBL:ALS37226.1, ECO:0000313|Proteomes:UP000067523};
RN [1] {ECO:0000313|EMBL:ALS37226.1, ECO:0000313|Proteomes:UP000067523}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 26678 {ECO:0000313|EMBL:ALS37226.1,
RC ECO:0000313|Proteomes:UP000067523};
RA Shamseldin A., Moawad H., Abd El-Rahim W.M., Sadowsky M.J.;
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
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DR EMBL; CP013655; ALS37226.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0U2WYV9; -.
DR STRING; 118060.ATZ35_08660; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000067523; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:ALS37226.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}.
FT BINDING 63..70
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 554 AA; 59662 MW; 4D2B044CFDB0F178 CRC64;
MVKSDIEIAQ ETTMLPITKV AEEIGLNEEQ YELYGKYKAK IAVEKIKADK NGKVILVTAI
TPTPAGEGKT TTVVGLGDAL NRIGKDAIIA LREPSLGPVF GIKGGAAGGG YAQVVPMEDI
NLHFTGDFHA IGAANNLLAA IIDNHIFQGN ELGIDNRRIT WKRAVDMNDR QLRHIVDGLG
ARVNGVPRED GFEITVASEI MAILCLSNDL DDLKENLANI IIGYTYENQP VTARDLNAQG
AMTALLKEAI KPNLVQTLEN NPALIHGGPF ANIAHGCNSV IATNTARKLA DYVVTEGGFG
ADLGAEKFID IKCRKSGIRP STVVVVATVR ALKMHGGIPK DQLGVENVSA LKEGLPNLLK
HIENTTTVFG LPTVVAINKF PTDTEAELKL VEEECQRRNV NVVLSDVWAH GGAGGEELAR
EVIRLADQEN NFSFAYPDEL PIKEKISAIV EKVYGGTAVR YEAIAEREIA KLEKLGFGNL
PICMAKTQYS FSDDQTKLGR PTDFTITISN IKISAGAGFI VAYTGTVMTM PGLPKKAAYE
KIDVDSDGKI TGLF
//
