ID A0A0U2XXA7_9BURK Unreviewed; 424 AA.
AC A0A0U2XXA7;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=glucarate dehydratase {ECO:0000256|ARBA:ARBA00011973};
DE EC=4.2.1.40 {ECO:0000256|ARBA:ARBA00011973};
GN ORFNames=AT302_03485 {ECO:0000313|EMBL:ALS58980.1};
OS Pandoraea norimbergensis.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Pandoraea.
OX NCBI_TaxID=93219 {ECO:0000313|EMBL:ALS58980.1, ECO:0000313|Proteomes:UP000060277};
RN [1] {ECO:0000313|Proteomes:UP000060277}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 11628 {ECO:0000313|Proteomes:UP000060277};
RA Ee R., Lim Y.-L., Yong D., Yin W.-F., Chan K.-G.;
RT "Complete genome sequence of Pandoraea norimbergensis DSM 11628.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O;
CC Xref=Rhea:RHEA:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:30612,
CC ChEBI:CHEBI:42819; EC=4.2.1.40;
CC Evidence={ECO:0000256|ARBA:ARBA00001426};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- PATHWAY: Carbohydrate acid metabolism; D-glucarate degradation; 2,5-
CC dioxopentanoate from D-glucarate: step 1/2.
CC {ECO:0000256|ARBA:ARBA00005183}.
CC -!- SIMILARITY: Belongs to the mandelate racemase/muconate lactonizing
CC enzyme family. GlucD subfamily. {ECO:0000256|ARBA:ARBA00009938}.
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DR EMBL; CP013480; ALS58980.1; -; Genomic_DNA.
DR RefSeq; WP_058375923.1; NZ_CP013480.3.
DR AlphaFoldDB; A0A0U2XXA7; -.
DR STRING; 93219.AT302_03485; -.
DR KEGG; pnr:AT302_03485; -.
DR OrthoDB; 193563at2; -.
DR Proteomes; UP000060277; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd03323; D-glucarate_dehydratase; 1.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR InterPro; IPR034593; DgoD-like.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR034598; GlucD-like.
DR InterPro; IPR013342; Mandelate_racemase_C.
DR PANTHER; PTHR48080; D-GALACTONATE DEHYDRATASE-RELATED; 1.
DR PANTHER; PTHR48080:SF4; GLUCARATE DEHYDRATASE; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR SFLD; SFLDS00001; Enolase; 1.
DR SFLD; SFLDG00055; glucarate_dehydratase; 1.
DR SMART; SM00922; MR_MLE; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 171..265
FT /note="Mandelate racemase/muconate lactonizing enzyme C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00922"
FT ACT_SITE 193
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634598-1"
FT ACT_SITE 320
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634598-1"
SQ SEQUENCE 424 AA; 47053 MW; 3C93F55ECC5475E7 CRC64;
MKIQRITITP IAFRDPPLLN AAGIHEPYAL RTIIEIETDN GHVGLGETYG DAPVLTLLQN
TREHLIGMDP FDTNGLRARV AAVVARGVGG ERVEYELAPG TDPRKDAEKI YSAFEVPFLD
LQARHLGIPL VELLGGAVRN EVQYSAYLFF KYAQHIDNPY APDPWGETLD ATQLVAQART
MIDTYGFGSI KLKAGALDPN EEVKCLKALK RAFPDKPLRI DPNGNWSLAT AIRMGRELEG
DLEYYEDPTP TLEGMAELHR ATGMPLATNM VVTDLRQFRE NVRVNGAQII LSDHHYWGGL
RDTQVLARMC ETFDLGLSMH SNSHLGISLM AMTHLAASVP RLSYACDTHY PWQAADEEVV
RGGKIAIRNG AVALTKAPGL GLEIDQDQLA KLHEQYLRCG VRTRNDAVQM RKYQPGWSGK
APRF
//
