ID A0A0U2YDW4_9BURK Unreviewed; 762 AA.
AC A0A0U2YDW4;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE SubName: Full=Malate dehydrogenase {ECO:0000313|EMBL:ALS62981.1};
GN ORFNames=AT302_02530 {ECO:0000313|EMBL:ALS62981.1};
OS Pandoraea norimbergensis.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Pandoraea.
OX NCBI_TaxID=93219 {ECO:0000313|EMBL:ALS62981.1, ECO:0000313|Proteomes:UP000060277};
RN [1] {ECO:0000313|Proteomes:UP000060277}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 11628 {ECO:0000313|Proteomes:UP000060277};
RA Ee R., Lim Y.-L., Yong D., Yin W.-F., Chan K.-G.;
RT "Complete genome sequence of Pandoraea norimbergensis DSM 11628.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: In the N-terminal section; belongs to the malic enzymes
CC family. {ECO:0000256|ARBA:ARBA00007686}.
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DR EMBL; CP013480; ALS62981.1; -; Genomic_DNA.
DR RefSeq; WP_058379836.1; NZ_CP013480.3.
DR AlphaFoldDB; A0A0U2YDW4; -.
DR STRING; 93219.AT302_02530; -.
DR KEGG; pnr:AT302_02530; -.
DR OrthoDB; 9805787at2; -.
DR Proteomes; UP000060277; Chromosome.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10950; -; 1.
DR Gene3D; 3.40.50.10750; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR012188; ME_PTA.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR042113; P_AcTrfase_dom1.
DR InterPro; IPR042112; P_AcTrfase_dom2.
DR InterPro; IPR002505; PTA_PTB.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR Pfam; PF01515; PTA_PTB; 1.
DR PIRSF; PIRSF036684; ME_PTA; 1.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|PIRSR:PIRSR036684-2};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|PIRSR:PIRSR036684-3};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 19..152
FT /note="Malic enzyme N-terminal"
FT /evidence="ECO:0000259|SMART:SM01274"
FT DOMAIN 164..401
FT /note="Malic enzyme NAD-binding"
FT /evidence="ECO:0000259|SMART:SM00919"
FT ACT_SITE 95
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-1"
FT BINDING 77..84
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 137
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 138
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 163
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 288
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
SQ SEQUENCE 762 AA; 82241 MW; 39E290C42840D71B CRC64;
MSNNNDHQAA LEYHQFPTPG KISVTASKPL VTQRDLALAY TPGVAIPCQE IAADPAKAFN
YTARGNLVGV ITNGSAVLGL GNIGALASKP VMEGKAVLFK KFAGIDVFDI EITESDPDKL
VEIIASLEAT FGGINLEDIK APECFTVERK LRERMKIPVF HDDQHGTAIT VSAAFINGLK
VVGKDIKEVK VVTSGAGAAA LACLDLMVDL GLPIENVWAT DIDGVVYEGR AVGMDPDKAR
FAQKTDKRTL AEVIDGADVF LGLSAGGVLK PEMLKTMGPR PLILALANPT PEIFPEDARA
ARDDVVLATG RSDFPNQVNN VLCFPYIFRG ALDVGATTIT RNMEIAAVNA IAALAQEEPN
DSVATAYGAY DLSFGPEYLI PKPFDSRLIV RIAPAVARAA MEDGVATRPI ADFDAYADQL
QQFVYHSGAF MKPIFSAAKQ LVRDGGKARI VFAEGEEERV LRAVQVIVDE KLARPILVGR
PEVLLARIEK FGLRLRLGED VEVTNPYYDE RFHQYWTSYW ELRCRDGITK EMARVEMRRR
LTLIGAMMVR LGDADGMICG TVGAYHDHLR FVDEVIGKQA GANTYAAMNI LLLDKRTVAL
VDTHVNDDPT AEQIAEFTIA AAKQMSWLNL NPKVALLSRS NFGSGSAASG TKMRRVLDLV
SAQAPDLEID GEMHGDCALD EGLRSRILPH SRLKGAANLL VCPNVDSGNI AYNLLKTGAG
SNVAVGPFLL GVGAPVNVLT SSSTVRRIIN MTALTVIQAN RD
//
