ID A0A0U3DD24_9VIRU Unreviewed; 2005 AA.
AC A0A0U3DD24;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE SubName: Full=Replicase {ECO:0000313|EMBL:ALT57194.1};
OS Asian prunus virus 2.
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Tymovirales; Betaflexiviridae; Quinvirinae; Foveavirus.
OX NCBI_TaxID=351426 {ECO:0000313|EMBL:ALT57194.1, ECO:0000313|Proteomes:UP000203741};
RN [1] {ECO:0000313|EMBL:ALT57194.1, ECO:0000313|Proteomes:UP000203741}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bungo Q-1256-01 {ECO:0000313|EMBL:ALT57194.1};
RX PubMed=26741704;
RA Marais A., Faure C., Candresse T.;
RT "New Insights into Asian Prunus Viruses in the Light of NGS-Based Full
RT Genome Sequencing.";
RL PLoS ONE 11:E0146420-E0146420(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SIMILARITY: Belongs to the potexviruses/carlaviruses RNA replication
CC protein family. {ECO:0000256|ARBA:ARBA00008513}.
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DR EMBL; KT893294; ALT57194.1; -; Genomic_RNA.
DR RefSeq; YP_009204561.1; NC_028868.1.
DR GeneID; 26631417; -.
DR KEGG; vg:26631417; -.
DR Proteomes; UP000203741; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016817; F:hydrolase activity, acting on acid anhydrides; IEA:InterPro.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd23245; Betaflexiviridae_RdRp; 1.
DR CDD; cd22792; OTU_RDRP-like; 1.
DR Gene3D; 2.60.120.590; Alpha-ketoglutarate-dependent dioxygenase AlkB-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR027450; AlkB-like.
DR InterPro; IPR037151; AlkB-like_sf.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR003323; OTU_dom.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR008041; Peptidase_C23.
DR InterPro; IPR001788; RNA-dep_RNA_pol_alsuvir.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF13532; 2OG-FeII_Oxy_2; 1.
DR Pfam; PF05379; Peptidase_C23; 1.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
DR PROSITE; PS50802; OTU; 1.
DR PROSITE; PS51492; PEPTIDASE_C23; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022806};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000203741};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}.
FT DOMAIN 63..254
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000259|PROSITE:PS51743"
FT DOMAIN 804..899
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
FT DOMAIN 936..1048
FT /note="OTU"
FT /evidence="ECO:0000259|PROSITE:PS50802"
FT DOMAIN 1047..1137
FT /note="Peptidase C23"
FT /evidence="ECO:0000259|PROSITE:PS51492"
FT DOMAIN 1184..1507
FT /note="(+)RNA virus helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51657"
FT DOMAIN 1788..1895
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
SQ SEQUENCE 2005 AA; 226922 MW; DDEBF003798DA691 CRC64;
MALTYRSPIE EVLNRFTSEE QSRVSSTSVA RLTQVEVSNH KLFSFALSEK AKEKLIKVGI
YLSPFSFEPH SHPACKTLEN HILYNILANK LDNSFYAIGI KGSKVNFLKS RSKNLSMLEY
VNRVVTSADK YRYGSDFTTL SSKQIDNLCS KKSLSECGTL KELVPLVKMS KKRNFFLHDE
LHYWSPQDLM LFLDSVNPDH LVATVVFPPE ILAGAKESLN PWCYSFHRQG SKLTFFPDGV
QSESYTQPLS SGYILKASKI VCPSGQIYSV DLVYSAFAHH VVSITKGNLP GLERRFFSNF
EATGVKHLSG LSHSVRDCIP VSTDLISKIY RYIRTLKKPD LQSCMAKLSQ IVPNPTGVEI
KFTEDFSRLV MEIQPLKSLL MPDTAKLIGA LFASFLPFKM ARFFDCYKSS SLDKFIHDLE
PFSFSVPTEV INCNTMPAFE AISLGESMSP EEVIQILNRM EMGAFAGSCN LVGKFKYSNE
AYHCLHHVLD GCKPEVFKRL IRRVAGSFCN EFGVYRKSSY VASVAINVFQ RRSNLNLDFI
LGSDLNQKVV CIVRNFFSSK KSSQLIWYST NSMAWFRFSK LRANQKFLRS PIFPASMNCK
DLSTRKNLIL DFEVFGFCAG LRKLGSSGVS SVVPPKPVLG GFEFVPPGEE AHEDDIEVKS
NTMAKMFDPS PQAVLGIEGP AAGVHEIDPQ DQTLSPKAAD LEERNGLIFG CVPEPSLMNF
SFSSANQGDR LFANIVNENI VDVGILPPTE LKIPLDAKVT QINGRDCYFF TRCSCIDYGH
NKIKYTANNW FSGLDQFLSN EGTYYNACLL QVYRAGSGIG FHSDNEKVYH RSPIKTVNLC
GEADFLVKAK GRRDLGTVAR GHLFPGHYFT MDSNFQSYYQ HSVQDCSEGR VSLTFRHHVN
NVTGLPIKHT CGEFGDTEIL FDVLIRRSFT YAAKNFHTFP VPGDGSCFWH SMGALLGVDG
EELKKISARE IQKNEILLSN LSLRQQMANK QYAERESIAA FCRIQSIHLV VLLPGNNFSY
EFLPSQNPEV TQLFVKLTDE HFEPALPVNG CVVKSIAETL NQTEAKILSV IGRPTNRMIL
DNLVEGEGLN LEDLEAAFTI FGICARVSSE NGVFTLNRDG NLHANYELKS GHIMYLRKVP
NSQFAPSTPI QNFNGQGPDL FGKGARNLEE SFLNGTTGVL CDRTVNLQKN WLTEGRRLVK
DERNLGVVLG TFGSGKSTLF KRFIVKNPSR SIVFVSPRRS LADQIKEDLG LNSKRGRSLR
VRVLTLESFI KAVFTFKAAS VVLDEVQLYP PGYLDLVMLC LSGNCQIYLS GDPCQSDYDS
AKDRALFDGM KGDIFEVLEG KSYKFNASSR RFQSQMFVGR LPCRLDSKDM VGDEDFHWLE
SIEAAAEVSN TEYDVVLVSS FEEKKIIWAH LGRDVEVLTF GESTGLTFNR GIILISHEST
LTGERRWITA LSRFRLNIIF VNLVGNNIED VCQVFHDRTL FKFLNRTATI GDIIKQLPGN
PELTNDFGDK VGKSEGVMED KLAGDPWLKT EIDLLQDEDQ EMVELAEEVK NEPWFKTHLP
LFELESIRAN WVHKIMNREY REVRCGTEVS TQFPEDHPNK YKVTLANAAE RFEAIYPRHK
GSDSVTFLMA VKKRLSFSRP SVESAKLNRA KPYGKFLLHE FLKRVPLKTG LDSALFARSK
REFEEKKTSK SAAVIENHSG RSCRDWLADV GFIFMKSQFC SKWDNRFRDA KAGQTLACFH
HSILCRFAPY MRYIEHKLQE VLPANLYIHS GKNLEDLNRW VIENSFTGMC TESDYEAFDS
SQDHYILSFE IEVMKHLQLP WDLIEDYVYI KTHLGSKLGN FSIMRFTGEA STFLFNTMAN
MLFTFLRYEL NGSEAICFAG DDMCSNRRLR VSNKHEGFLS KLRLKAKVQF TVKPTFCGWN
LCLDGIFKRP QLVLERLCVA REKDNLANCL DSYAIEVGYA FALGEKVLQY MDEEAMQNHY
NCVRFIVKHS HLLKSSVRDL FLQNM
//
