ID A0A0U3PUM0_9ACTN Unreviewed; 988 AA.
AC A0A0U3PUM0;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=AS200_27720 {ECO:0000313|EMBL:ALV35413.1};
OS Streptomyces sp. CdTB01.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1725411 {ECO:0000313|EMBL:ALV35413.1, ECO:0000313|Proteomes:UP000068029};
RN [1] {ECO:0000313|EMBL:ALV35413.1, ECO:0000313|Proteomes:UP000068029}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CdTB01 {ECO:0000313|EMBL:ALV35413.1,
RC ECO:0000313|Proteomes:UP000068029};
RA Tian Y., Zhou G., Yang H., Lu X.;
RT "Complete genome sequence of the Streptomyces sp. strain CdTB01, a
RT bacterium tolerant to cadmium.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP013743; ALV35413.1; -; Genomic_DNA.
DR RefSeq; WP_058925023.1; NZ_CP013743.1.
DR AlphaFoldDB; A0A0U3PUM0; -.
DR STRING; 1725411.AS200_27720; -.
DR KEGG; scx:AS200_27720; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000068029; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000068029}.
FT DOMAIN 11..301
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 302..577
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 675..697
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 32..39
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 988 AA; 109328 MW; BCB692810B167C6E CRC64;
MNVDVEQALG GWLTPEQYGA AVAPEDEVLA LAGAGSGKSR TLAGRVAYKI ANGASPESVV
AFTFTEKAGE QIKTRVAQAL KVCGVDPLLV GGMYIGTIHA WCRRVLGDMD ARYRQFDVLD
EMQFLVYLIS RYPKLNINPL RDAHPTAKGN RRGYFETLKE LSRAWHMMND ELLDPAQIAA
EDPALGEALE RLRDQMEQDN FIDFSLMQRL VVEALEAQEP GAVQALALLR HVLVDEYQDV
NVVQERLIGL MHQNSETLFV VGDDDQAIYG WRGADVTNIL EFNRRYPTAA VHKLLTNFRS
TSAIVEAAGD FAAQELGPSR LPKQLHAAAN HDPREFAVLN FPVRAVEAEF VAERIDELLG
TAYEESDGTV RGLTPADFAI LMRSTGQPES DGTKRHTAYI QALDARNIPY DLNAGGQLFD
RAQPAAVRSA FELLRDAMPD RTQVEDLFHS QLKKAFPQAD LSRVIRVFAD WGRRIHAPIA
PGLPRQRLFP QQLLHDLLEA LDVANSGFDD GVMADLGTFS RILQDIETVY LSIDGKPRFG
ELLNFLQNVA GDGYETDNQM MARPDAVTVS TVHKAKGLEF PVVFVVDVEN GRFPGRRRSY
DGWIPETLIQ QAIDNGSYQT GPHDQARLFY TAITRAERFL YVTGSALLPG GKRLTKTSPY
AVRLNHPEIR RDLLDPAAPP RVVPPVEAQR RRGDESVLPT TYSQIHTFMR CPHAYKLSVV
NGFSPPIPEL FGFGRAVHAA VGKLHQAYKD KAPTSDEARA IAENMFHLKH VAPSSDPVDR
PGAYERAKQK SSDIVSDYVD KYGTDFELKR QVELPFEIPV EGAVISGAID LLLSVDDADT
IHDAKVIDFK TMAGGPEPTN NPDLNWEDLS LQVQLYGRGA EQVHGMNART GAVHLLKDGQ
RVQVPVDADA VDAAVANVEW AVSRITDGDF PQRGSRTKCG DCDFAQICSK KLQPFKTEEE
PPDLHLPDGT NGKRFLKVSA FSRTGPNE
//
