ID A0A0U3QEH9_9ACTN Unreviewed; 597 AA.
AC A0A0U3QEH9;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=Neutral metalloproteinase {ECO:0000256|RuleBase:RU366073};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU366073};
GN ORFNames=AS200_40875 {ECO:0000313|EMBL:ALV37725.1};
OS Streptomyces sp. CdTB01.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1725411 {ECO:0000313|EMBL:ALV37725.1, ECO:0000313|Proteomes:UP000068029};
RN [1] {ECO:0000313|EMBL:ALV37725.1, ECO:0000313|Proteomes:UP000068029}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CdTB01 {ECO:0000313|EMBL:ALV37725.1,
RC ECO:0000313|Proteomes:UP000068029};
RA Tian Y., Zhou G., Yang H., Lu X.;
RT "Complete genome sequence of the Streptomyces sp. strain CdTB01, a
RT bacterium tolerant to cadmium.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Extracellular zinc metalloprotease.
CC {ECO:0000256|RuleBase:RU366073}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU366073};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU366073}.
CC -!- SIMILARITY: Belongs to the peptidase M4 family.
CC {ECO:0000256|ARBA:ARBA00009388, ECO:0000256|RuleBase:RU366073}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP013743; ALV37725.1; -; Genomic_DNA.
DR RefSeq; WP_058927305.1; NZ_CP013743.1.
DR AlphaFoldDB; A0A0U3QEH9; -.
DR STRING; 1725411.AS200_40875; -.
DR KEGG; scx:AS200_40875; -.
DR OrthoDB; 291295at2; -.
DR Proteomes; UP000068029; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09597; M4_TLP; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 3.10.450.490; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR023612; Peptidase_M4.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR001570; Peptidase_M4_C_domain.
DR InterPro; IPR013856; Peptidase_M4_domain.
DR PANTHER; PTHR33794; BACILLOLYSIN; 1.
DR PANTHER; PTHR33794:SF1; BACILLOLYSIN; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF01447; Peptidase_M4; 1.
DR Pfam; PF02868; Peptidase_M4_C; 1.
DR PRINTS; PR00730; THERMOLYSIN.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Cell projection {ECO:0000313|EMBL:ALV37725.1};
KW Cilium {ECO:0000313|EMBL:ALV37725.1};
KW Flagellum {ECO:0000313|EMBL:ALV37725.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366073};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU366073};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU366073};
KW Reference proteome {ECO:0000313|Proteomes:UP000068029};
KW Secreted {ECO:0000256|RuleBase:RU366073};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU366073};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366073}.
FT SIGNAL 1..28
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT CHAIN 29..597
FT /note="Neutral metalloproteinase"
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT /id="PRO_5023076965"
FT DOMAIN 68..107
FT /note="FTP"
FT /evidence="ECO:0000259|Pfam:PF07504"
FT DOMAIN 249..400
FT /note="Peptidase M4"
FT /evidence="ECO:0000259|Pfam:PF01447"
FT DOMAIN 419..587
FT /note="Peptidase M4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02868"
FT ACT_SITE 394
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
FT ACT_SITE 490
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
SQ SEQUENCE 597 AA; 62414 MW; 8F76D86EBAC9DF08 CRC64;
MRRPHIRSVA VAVAVTTAAT GLAGTAFAGP DTGTHATAAD ASASAVVKAA ESAAFAHASA
TGVSHGDGLQ AQDVMIDPEG ARHVRFTRTH QGLPVLGGDL VVHLTRQLSY AGVTRAADHA
VKAAPASGAK LSAAEAEQKA ASVAKGDAGP AELVVDARDG ASALAYRVSV SDSTTTQGAG
SRTVVVDAVS GKVRSNLPDN DEFLSPKLLD TLRERGETSA PVTGTGARSA GLLAGSAAAA
TRYPVTANGT GKSLFVGKVP LKTTQTARTT FQLKDPTRYG SETRDAKGSY TESFGSGTKI
TTKNDVFGNG ATTSRNSAAA DAQYGITKTL DFYKKTFGRK GIANNSKPAK AMVHWGKKVA
NAFWDSDCGC MLYGDGDGDM FKKPLVALDV TGHELTHGVV DATAKLEPTR MDGQGNQYGE
PGSLNESLAD IFASNVEFAT NNATDKPDYL IGEKMGLAQK FLRRLDHPSL DKLEGTIDYW
APEVYDAEVH AGSGVSSHAY YLLAEGSGQK TIGGVKYDSP TFDGSTVKGI GRTKATAIYY
RALTRYMVST TDFHDARVAT LKAAQDLYGA NSVEYRTVDQ SWAAVNVTEA NTPADRH
//
