ID A0A0U5BNE4_9MICO Unreviewed; 1170 AA.
AC A0A0U5BNE4;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=Error-prone DNA polymerase {ECO:0000256|ARBA:ARBA00017273, ECO:0000256|HAMAP-Rule:MF_01902};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|HAMAP-Rule:MF_01902};
GN Name=dnaE2 {ECO:0000256|HAMAP-Rule:MF_01902,
GN ECO:0000313|EMBL:BAU32379.1};
GN ORFNames=MalAC0309_1527 {ECO:0000313|EMBL:BAU32379.1};
OS Microcella alkaliphila.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microcella.
OX NCBI_TaxID=279828 {ECO:0000313|EMBL:BAU32379.1, ECO:0000313|Proteomes:UP000218965};
RN [1] {ECO:0000313|Proteomes:UP000218965}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JAM AC0309 {ECO:0000313|Proteomes:UP000218965};
RA Shamseldin A., Moawad H., Abd El-Rahim W.M., Sadowsky M.J.;
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:BAU32379.1, ECO:0000313|Proteomes:UP000218965}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JAM AC0309 {ECO:0000313|EMBL:BAU32379.1,
RC ECO:0000313|Proteomes:UP000218965};
RA Kurata A., Hirose Y., Kishimoto N., Kobayashi T.;
RT "Microcella alkaliphila JAM AC0309 whole genome shotgun sequence.";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC translesion synthesis (TLS). It is not the major replicative DNA
CC polymerase. {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632, ECO:0000256|HAMAP-
CC Rule:MF_01902};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000256|ARBA:ARBA00007391, ECO:0000256|HAMAP-
CC Rule:MF_01902}.
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DR EMBL; AP017315; BAU32379.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0U5BNE4; -.
DR KEGG; malk:MalAC0309_1527; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000218965; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR HAMAP; MF_01902; DNApol_error_prone; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR023073; DnaE2.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01902};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|HAMAP-Rule:MF_01902};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01902}.
FT DOMAIN 63..130
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
FT REGION 1..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1170 AA; 128147 MW; 5FE9CFBA12B32820 CRC64;
MGWHNPPIPW SELERTLRSG NRPDPSLPEV GDGSDSPAWS TKRAPYRPGR VEQPDPETVV
PYAELHAHSS FSFLDGASSP EELLEEAARL GLTGLALTDH DGFYGVVRMA EAAEPYGLTT
VFGAELSLGL TKPQLGEPDP EGTHLLVLAR GQEGYHRLAA ALTDGQLAGG AKGRPVYDLE
VLAQHAGRGG DATAPGHWVI LTGCRKGAVR TALTAGDPNG QTVPSRASIA AAGRELDALV
ERFGRENVVV ELFDHGRPLD TVYNDALAAL AAERGLPVVA TGAVHYAHPG RYPLASAFAG
LRARRSLDDM DGWLPAAGTA HLRSGAEMQR LFARYPGAVD RSVELARELG FSLRQAKPKL
PKLPVPEGHT PMSWLRELVW QAVPQKYPDA SDADRARIAR ELEVIEQKDF PGYFLIVHDI
VRFARSRGIL CQGRGSAANS AVCYLLDITA VDSIYYKLPF ERFLSALRDE EPDIDVDFDS
DRREEVIQYV YETYGRRNAA QVANVISYRP KNAVRDMAKA LGYSTGQQDA WSRQVERWGA
VLSSDDHDIP DAVVDLAQQV LTYPRHLGIH SGGMVLTDRP VGEIVPIEHA RMTKRTVLQW
DKDDCAWMGL VKFDLLGLGM LAALQYAFDL IDDHLGERWE LATIPKEEQT TYDMLCRADS
IGVFQVESRA QMGLLPRLQP RKFYDLVVQI ALIRPGPIQG GAVHPFVKRK LGEEPVTYDH
PKLVEPLERT LGVPIFQEQL MQIAMAVGGC TGDDADLLRR AMGSKRGLER IESLRGTLYA
GMASNGITGR LADEIYARIQ AFANFGFAES HSLSFALLVY ASSWIKLHYP GVFLASLLRA
QPMGFYSPAS LTADAERHGV EVRGPCIVRS GVEAGLERLD LTVDDPFSPL SADARRAAPT
GLASCTHRVH PPVPEFRPDS RDGELEDLVP HRRDGELAVR MGLGAVSGLG KETAEKVIAE
RERYGPYRDL TDLVYRTGLT AAQLESLATA GAFEVWGMTR REALWAAGAA AQARPEYLPG
SVVAIQPPLF DDLEPAERMV ADLVTTGVAP GDHPVRYARE RLRARGVLSA RELRSAEPGR
RVEVAGVVTH RQRPATASGI TFLNLEDETG LANIICSVGL WGRYRRTVRE SRALIVRGIL
ERSPEGVVNV VADRVEALSL KATMPSRDFR
//
