ID A0A0U5NPC7_9CLOT Unreviewed; 293 AA.
AC A0A0U5NPC7;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Proline iminopeptidase {ECO:0000256|ARBA:ARBA00021843, ECO:0000256|PIRNR:PIRNR005539};
DE Short=PIP {ECO:0000256|PIRNR:PIRNR005539};
DE EC=3.4.11.5 {ECO:0000256|ARBA:ARBA00012568, ECO:0000256|PIRNR:PIRNR005539};
DE AltName: Full=Prolyl aminopeptidase {ECO:0000256|ARBA:ARBA00029605, ECO:0000256|PIRNR:PIRNR005539};
GN Name=pip {ECO:0000313|EMBL:CUX30855.1};
GN ORFNames=BN3456_01275 {ECO:0000313|EMBL:CUX30855.1};
OS Clostridium sp. C105KSO13.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1776045 {ECO:0000313|EMBL:CUX30855.1, ECO:0000313|Proteomes:UP000198973};
RN [1] {ECO:0000313|EMBL:CUX30855.1, ECO:0000313|Proteomes:UP000198973}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C105KSO131 {ECO:0000313|EMBL:CUX30855.1};
RA McClelland M., Jain A., Saraogi P., Mendelson R., Westerman R.,
RA SanMiguel P., Csonka L.;
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Releases the N-terminal proline from various substrates.
CC {ECO:0000256|PIRNR:PIRNR005539}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of N-terminal proline from a peptide.; EC=3.4.11.5;
CC Evidence={ECO:0000256|ARBA:ARBA00001585,
CC ECO:0000256|PIRNR:PIRNR005539};
CC -!- SIMILARITY: Belongs to the peptidase S33 family.
CC {ECO:0000256|ARBA:ARBA00010088, ECO:0000256|PIRNR:PIRNR005539}.
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DR EMBL; FBWL01000160; CUX30855.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0U5NPC7; -.
DR STRING; 1776045.BN3456_01275; -.
DR OrthoDB; 9775557at2; -.
DR Proteomes; UP000198973; Unassembled WGS sequence.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR002410; Peptidase_S33.
DR InterPro; IPR005945; Pro_imino_pep.
DR NCBIfam; TIGR01250; pro_imino_pep_2; 1.
DR PANTHER; PTHR43194; HYDROLASE ALPHA/BETA FOLD FAMILY; 1.
DR PANTHER; PTHR43194:SF5; PIMELOYL-[ACYL-CARRIER PROTEIN] METHYL ESTER ESTERASE; 1.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR PIRSF; PIRSF005539; Pept_S33_TRI_F1; 1.
DR PRINTS; PR00793; PROAMNOPTASE.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 3: Inferred from homology;
KW Aminopeptidase {ECO:0000256|PIRNR:PIRNR005539,
KW ECO:0000313|EMBL:CUX30855.1};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR005539, ECO:0000313|EMBL:CUX30855.1};
KW Protease {ECO:0000256|PIRNR:PIRNR005539};
KW Reference proteome {ECO:0000313|Proteomes:UP000198973}.
FT DOMAIN 28..277
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000259|Pfam:PF00561"
FT ACT_SITE 104
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005539-1"
FT ACT_SITE 244
FT /evidence="ECO:0000256|PIRSR:PIRSR005539-1"
FT ACT_SITE 271
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005539-1"
SQ SEQUENCE 293 AA; 33234 MW; 5ADCBCF24234D8FB CRC64;
MNVIEGYMPY RGYKTYYRIV GECKGNKKPL VLLHGGPGST HNYFEVLDRL ADEGRAIIFY
DQLGCGNSYV EGHPELWCSD TWDNELIELR KHLKLDQVHL LGQSWGGMLA IEYLCDYKPE
GVQSVILSST LPAAELWAHE QHRLIKLMSG EDQEAIAKAE KSGNFDDPDY LAANDRFMLL
HCAGEVTEDS PECLRRPKKS GAEAYVAGWG PNEYNPTGSL GGWDYRDKIG GIKEPALIIS
GTNDLCTPLV AKTMYDAIPN SRWELFDGCR HMCFVEENDK YCRLVNEWME KYD
//
