ID A0A0V0S8K5_9BILA Unreviewed; 4388 AA.
AC A0A0V0S8K5;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN Name=HUWE1 {ECO:0000313|EMBL:KRX23049.1};
GN ORFNames=T07_9508 {ECO:0000313|EMBL:KRX23049.1};
OS Trichinella nelsoni.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Enoplea; Dorylaimia;
OC Trichinellida; Trichinellidae; Trichinella.
OX NCBI_TaxID=6336 {ECO:0000313|EMBL:KRX23049.1, ECO:0000313|Proteomes:UP000054630};
RN [1] {ECO:0000313|EMBL:KRX23049.1, ECO:0000313|Proteomes:UP000054630}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ISS37 {ECO:0000313|EMBL:KRX23049.1};
RA Korhonen P.K., Edoardo P., Giuseppe L.R., Gasser R.B.;
RT "Evolution of Trichinella species and genotypes.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRX23049.1}.
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DR EMBL; JYDL01000027; KRX23049.1; -; Genomic_DNA.
DR EMBL; JYDL01000027; KRX23052.1; -; Genomic_DNA.
DR STRING; 6336.A0A0V0S8K5; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000054630; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.720.50; -; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR003903; UIM_dom.
DR InterPro; IPR004170; WWE-dom.
DR InterPro; IPR037197; WWE_dom_sf.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF06012; DUF908; 2.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF14377; UBM; 3.
DR Pfam; PF02825; WWE; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR SUPFAM; SSF117839; WWE domain; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50330; UIM; 1.
DR PROSITE; PS50918; WWE; 1.
PE 3: Inferred from homology;
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000054630};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1744..1820
FT /note="WWE"
FT /evidence="ECO:0000259|PROSITE:PS50918"
FT DOMAIN 4052..4388
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 764..826
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1423..1465
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1540..1568
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2004..2027
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2424..2534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2733..2783
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2923..2965
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3396..3442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3724..3798
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3922..3954
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 799..826
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1423..1456
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1541..1565
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2424..2516
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2733..2751
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2923..2938
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3396..3438
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3732..3750
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3751..3798
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 4355
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 4388 AA; 491821 MW; 278A742DF22EB80A CRC64;
MYMTLVPMLS VQSYLNKAVN CGNSEEELEM KIDVTSVRKQ TAQAPSDCLK QISEILSADE
SSIADFLINV KTWSWSKSEL CHWIPVLNRF DDFLAQACVR KEDNEWRLHC DQRQEKLVIG
ILQFTALLFE NTFSRDIYNS MEHIVVLLES SSISILAATL NLINVFSKRS TFLSRLSKDL
HEELKARMLA IVETWGGPEF GLTLSICCRR DVEIPSALTT LTFDVDDDEI NLTNPKNDND
KGSSRRTIRF VEVHKQKRTP AQWMQELISK GITLPADKKF LLFHRLRVAY AFGNFETRLL
MVICRLKAVA ISVYISSFTD FEYRLLNTGL VIQMLDVLKL PDLPELMAVR AEVFRTLGTF
FSHVRTGIWT EVVDPLGLTF FHGFLPTLTR SCVNELLEHS SNSPVKYPHY FSTALFSFLY
YLAKYDRNGA QALTTSGTVE VLLKVVSSSA ARGEGLTFVT RAVRIIEMTL TPTDSMTFQN
LNGIQTFIHR LSDEIDHCRC ALGHPLSSSD DSSIHAPGTS GSNVPQQSND ALKGKPLLPF
AISSDAQALS FQSAALIKSL LSFMKKIIQD PQFSESVRHI MDSNFSNCLS HIIQNSLFYG
PSLFLLAMSC VASFILQEPS QLSQLQERGL TTEILEALFR NEIPTSREIV SSLPNIFSAL
CLNERGVSQF LSYSPFKTLF TLLISPNYLN ALRRVRSLED VNNTAFILGS MIDQLLRHQP
RLKSDAIHAV VEVCICDHVD ADDRLLRQLA VMYKCRGVTC AEEADVSGRN RRRPQFPTEH
PLPFSRLGNN DSDQSDPDDS ESGPVPSLVL SSPNGNSSEN NSNRNDLSNE QVIPLVDYFE
NVLKFLHGLF NRNQNDEHAR LFLNLGGMYS LVDLIGCGNI PVDYMGGQLY YLLATICRQL
YVVTHNPLIF EGIFSSALDR ARAVKNIFSY HSKNADRPDV RHSVLVKHFP DGVQTVESTV
YSSSQFSAVF DLFNHIYVLS CMLTTIFQQG ENRAIILKTV NEFPLVETLI KVHLVLLLEM
GCVASATEEA VRRNGPVSVS QFMKDGHTLD AKSFPGANDI LSMQLLRRAV MNELPNDSTT
CNHSKKSTAL RCFPMELYFC ERLILQLKFF FGRLAKLTQT NRPGIPGRRF AQFADRTAVT
VEGDKAMTHL VKTLCSINKV TISSKNLDAC LYICTATLEL LSDIFLDYEH LHVTPVIEFF
QRVSTFQMLQ NLSNIVDSDR LWLGKDKYGK SCGSDARGFI SDVLVLIDKL IRGKHFDHRS
ESEGRSSFPN TPHPVIISSL SKFMYKALIS LWHRLPSSPK LKSDEALSAL SRITLHALRY
ESRKIEERKS SEKSSVVQAE VEVIPEISQI VNPVHLQQLM DVGFSQQLAT EALSRLPNVD
QAAEYCLYAS SNRALVQHGN QADVGDDEEQ LRQAIALSLM TMSSPGGEQQ QQRPPETQAA
AASSSSSSSN QRGVDFVPEI RQQPSREEIN ATIEDLLTGT GTQMMDIAES SLASIGSFPP
LFEQIERQLE MEMEMELELE MEPRTSGRNI LAPVISAGSN AAQMPPASSS HKTQLPKQAS
KAASQSVVPK EHDERSEAFL RFAEKDLLPS SILLLKRSPK MAAALTPLFS IVITLFGFKS
VKTYLLIFCN RLCSVVTTTM NEIEQMMEDN SEVDRSTTIL SDSRFEFEEL STLLNLLHRL
FVQFQVPMAI MLHETKLLAK FEEFIVLIGL KKKNGTFLSF MSKRSQAWLA YILRILELHV
YTCEVVDRLL AIPKCRNYVW KFWDRQWYCY SPSVNRKIND AFMAGDQYVY FLQGRRTYRI
DFFLMIQFCY ETNIIRKVIC LRPENSDKNS ADNSTPVTYC YHPFLNYVDM ATFKKEDLGS
CDKEILLNVA VEMIGSSVHA ETLRGILRII LLLTRDHSRT KYIVFHGTIK RLFALDESQK
FVGVEDMVML LLRHIIEDPD YLQNLMEKII ASLLTGHMAI SNYTSTELKE FSCLLCLLEP
FACRDMNLFA KAFKRTAKIN IASNENRTNA NNTSNNSGSN SNNNSNNNAG AAAPSTVCLE
LSCATPSVVN SRSPHLKPAA FELIKTLLEY IMTKQKSSVS SVVKDSRAEM KPSSSRLFQR
ESAEGESQLA GSLLFPKPLV MLYLAEIIQC YPDAASVVVN FRLNESGSLN TSDNRSPLAH
ILDVLIEGEY EEWKCKGQTF VDGVKAVFGS LAACVHCEES QVAVVSEIKA ALARALALNN
NERKHKILDN LMSLLCHIVE VCPLSEVQIP KDSKLPPPQN TRFKSILRWF VKKRLLVDLA
RIPHSLDLSD GQSVLTVNCM LKALDEISRQ HFYPSRSRST GTPAINYLTS NGQAGRRSLI
RISETVDSSG HDYVFLNLAS ANGRGNNLFT VFDSEFPTLR SLDGDFSSDA FFDREDTIEV
AQEPPQGNGN DNFPEFIVHN LAADDDEEDD DEDDEDDEDE DVEEEEIDIN EEVGEDEEEE
EADHGDDHEV GEEEEEEEAD NDDSEYEAVE DDVNEEDDDD DDDDDDDDED EDDELANSFR
DTSYVEENNN NAISSNDLAG SIGALGDLLR QTLSSNSNGV RDVINQLSSE GREERWHHDV
PSITIEPDVE FGYRTLGRNR GGQHRSALPR IVQLNRQSAF RIRVTEPMPI VDTDDIFWRD
FFSEAVRPAE RFVMFQRSLT DTLRSINFGS RLNVTNSSEA NMPMTTEICG FFTRIMQEGS
LLEMDSTYLA WLGVREDILK ALEKRRDDEL SRKQAEALSA ADSEDKSKKS ENNNTSGTAS
QDEPGQMRAM VTGDHPRDSA NSSQLAESFI NQDRNDIEQE ISWVSVADGQ VLFEGSRQQV
VDNSQRLFQQ QQAEQEQQAV ANNDANPEIP IVANNAFLEA LRQAQEHFET PGRLDMPDPD
ALQEACNMLL MTGGLSETPE QRQDQQLDPL PEPDLVQGRV AAEEQQQLQQ QEPQENVESN
ADAAVAADAT TAAPETAETE ELTEEQRRIR DILGDTPIPE GVDRAFLAEL PENIRAEVIA
EQWRLLRLQQ QSRSAEGTDG SAPPAISMDF LVALPPELQE EVIAQHRISS QQQNNPDGQM
DLATFFETLP APLRRQILSD ADESQMAFFP SHIAAEARHI QLTFNNRETG RPRHYHVVRT
GRNGQRGDNS VRPRHGIRLA RRPVPADMRS LRQQLANWNR DTASYTRTQF GGHTGSKSAT
TNTTLSTRSS ALFDRESLTC LLVLLFVDDS RLSTARLTRI LRTVCAHPGT REWLLRALVS
ILQRTWMSVD LIGGSTLGGV RPSDPEFGDL SSYTFSPTPS RSTVVARSSH RRNEAAGAAG
GLHLSSANSE NKGWLSMTMT SKLGNCVEVF RISKPTASGG KRALLFPQLT PVLLHPLVVP
AVTTAVINTI AAIAKSYSHS FFPQLRKLLN SQSNAANTSD SASASVAEAG STAQQSGAAR
QGDEQQPQSA SKTPTLRRRS SSAVDRIRSD FWDILTKLNN ASSANSRTKH SPKIVSFGFP
DTTAQCGVED SLFGQLVLFL RHPVIVERQG LQSILLSTLQ HVTSTSLAQI SDTILDQGVF
LEEQLKLLID VLVRDSSSGD NRNDARLILV RCAAALGPAT RRRIFELLFE AARELAGALR
KELIVLLEEM RSLKNNEPGT SAQFVASSSS SSALLPARGR MIDRFTGTTL VVSSTSPAKA
PNFACSQELQ CPSMELLSKK NSMQTCLLRV INSILSMKHS MKKFDERDPN FEQDVTSFDF
ELQDSEEVID ESDMSAGDRD RDRRNGSSQE EGRQETTAAV EPAPATTTTT AGTDASSTTV
TASSAATTTT TTSDESSTAT VAAPVTPVTS AVVEVSDSNA PFVTVTSTSI TALSSGDGSS
NNELVGEETQ RPVFELEVQP LTVSMQVDNL WDLLSECLTL LDESADPHAV LILQPAVEAF
FIIHGDDKKH MNELCTLLQS STRPDSDTLT ESSASNSAQP FSDMTVSSND AMKSSDPHFR
NLPLDVQMFI RFAEKHRTVL NQIIRQSKTP LNTGPFSVLI NYTRILDFDI KRRYFRLELE
RVDQTFRADD TPVRVKRDRI FQDSFRELFR LRPSEWKSRF YILFEGEEGQ DAGGLLREWF
SVITREIFDP NYALFMTASG DSVTYTINPS SYINREHLQY FKFIGRLIAK AIYDNKLLDC
YFTRAFYKHI LSKPVKYTDI ESEDPSFYQS MVFLLENPVS TLGYDLTFSL EVEEFGVREI
RDLKPEGRTV LVTDANKMEY VRLVCQMKMT GAIRQQLNAF LEGFYDIIPR RLISIFNEQE
LELLISGLPI IDIDDLQQNT EYYKYNKNSL QIQWFWRAVR SFDHAERAKL LQFVTGTSRV
PLQGFAALPG MHGAQRFQIH RDDRSVDRLP AAHTCFNQLD LPPYETYDKL RHMLLLAINE
CSEGFGFA
//
