ID A0A0V1AFH7_9BILA Unreviewed; 402 AA.
AC A0A0V1AFH7;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 08-NOV-2023, entry version 19.
DE RecName: Full=Protein-tyrosine sulfotransferase {ECO:0000256|ARBA:ARBA00013262, ECO:0000256|RuleBase:RU365018};
DE EC=2.8.2.20 {ECO:0000256|ARBA:ARBA00013262, ECO:0000256|RuleBase:RU365018};
DE Flags: Fragment;
GN Name=tpst-1 {ECO:0000313|EMBL:KRY22979.1};
GN ORFNames=T12_13377 {ECO:0000313|EMBL:KRY22979.1};
OS Trichinella patagoniensis.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Enoplea; Dorylaimia;
OC Trichinellida; Trichinellidae; Trichinella.
OX NCBI_TaxID=990121 {ECO:0000313|EMBL:KRY22979.1, ECO:0000313|Proteomes:UP000054783};
RN [1] {ECO:0000313|EMBL:KRY22979.1, ECO:0000313|Proteomes:UP000054783}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ISS2496 {ECO:0000313|EMBL:KRY22979.1};
RA Korhonen P.K., Edoardo P., Giuseppe L.R., Gasser R.B.;
RT "Evolution of Trichinella species and genotypes.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the O-sulfation of tyrosine residues within acidic
CC motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as
CC cosubstrate. {ECO:0000256|RuleBase:RU365018}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3'-phosphoadenylyl sulfate + L-tyrosyl-[protein] = adenosine
CC 3',5'-bisphosphate + H(+) + O-sulfo-L-tyrosine-[protein];
CC Xref=Rhea:RHEA:16801, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:11688,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:46858, ChEBI:CHEBI:58339,
CC ChEBI:CHEBI:58343, ChEBI:CHEBI:65286; EC=2.8.2.20;
CC Evidence={ECO:0000256|ARBA:ARBA00000411,
CC ECO:0000256|RuleBase:RU365018};
CC -!- SIMILARITY: Belongs to the protein sulfotransferase family.
CC {ECO:0000256|ARBA:ARBA00009988, ECO:0000256|RuleBase:RU365018}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRY22979.1}.
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DR EMBL; JYDQ01000006; KRY22977.1; -; Genomic_DNA.
DR EMBL; JYDQ01000006; KRY22979.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0V1AFH7; -.
DR STRING; 990121.A0A0V1AFH7; -.
DR Proteomes; UP000054783; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008476; F:protein-tyrosine sulfotransferase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR026634; TPST-like.
DR PANTHER; PTHR12788:SF10; PROTEIN-TYROSINE SULFOTRANSFERASE; 1.
DR PANTHER; PTHR12788; PROTEIN-TYROSINE SULFOTRANSFERASE 2; 1.
DR Pfam; PF13469; Sulfotransfer_3; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000054783};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365018};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 27..46
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KRY22979.1"
SQ SEQUENCE 402 AA; 45599 MW; 14412F9B7D0189FA CRC64;
LKAAAAATTT TTTTFGEVWM RRGLHRLFLL IALPVGLLCF LAYQLLLDSG GSCRQSVVPS
TLKIVDSISS STSQIERSST FGNFSYDNTL PLIFIGGFPR SGTTLIRAML DAHPDIRCGE
ETRLLPRILG MRAHWKNEKV EWRRLIEAGV DDEVIDAAVS AFIMEVIVRH GPLAPRLCDK
DPFLMKFAVY LSELFPNAKF VFMIRDGRAA VHSMITRRVT IGGFDLTDIR QCLSKWSLSV
AQMYNECYNI GARRCFPMYY EQLVLNPEKW LRELLAFLEV PWSDHVLNHE KYIGREISLS
KSERSTDQVM KPVYTSSLDS WVGKFPEDIL KDMPKIAPML DFLGSIFPFT IFSSYDPKVY
KPDYGKAGEF ITRKLNLFNG TESFKQWLKK VIDGMKPAAV KS
//
