ID A0A0V1MJS3_9BILA Unreviewed; 2002 AA.
AC A0A0V1MJS3;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=E3 ubiquitin-protein ligase {ECO:0000256|RuleBase:RU366018};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU366018};
GN Name=Ubr3 {ECO:0000313|EMBL:KRZ71875.1};
GN ORFNames=T10_12513 {ECO:0000313|EMBL:KRZ71875.1};
OS Trichinella papuae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Enoplea; Dorylaimia;
OC Trichinellida; Trichinellidae; Trichinella.
OX NCBI_TaxID=268474 {ECO:0000313|EMBL:KRZ71875.1, ECO:0000313|Proteomes:UP000054843};
RN [1] {ECO:0000313|EMBL:KRZ71875.1, ECO:0000313|Proteomes:UP000054843}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ISS1980 {ECO:0000313|EMBL:KRZ71875.1};
RA Korhonen P.K., Edoardo P., Giuseppe L.R., Gasser R.B.;
RT "Evolution of Trichinella species and genotypes.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Ubiquitin ligase protein which is a component of the N-end
CC rule pathway. Recognizes and binds to proteins bearing specific N-
CC terminal residues that are destabilizing according to the N-end rule,
CC leading to their ubiquitination and subsequent degradation.
CC {ECO:0000256|RuleBase:RU366018}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|RuleBase:RU366018};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|RuleBase:RU366018}.
CC -!- SIMILARITY: Belongs to the UBR1 family. {ECO:0000256|ARBA:ARBA00009750,
CC ECO:0000256|RuleBase:RU366018}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRZ71875.1}.
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DR EMBL; JYDO01000088; KRZ71875.1; -; Genomic_DNA.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000054843; Unassembled WGS sequence.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR GO; GO:0071596; P:ubiquitin-dependent protein catabolic process via the N-end rule pathway; IEA:UniProtKB-UniRule.
DR CDD; cd19673; UBR-box_UBR3; 1.
DR Gene3D; 2.10.110.30; -; 1.
DR InterPro; IPR044046; E3_ligase_UBR-like_C.
DR InterPro; IPR039164; UBR1-like.
DR InterPro; IPR003126; Znf_UBR.
DR PANTHER; PTHR21497:SF39; E3 UBIQUITIN-PROTEIN LIGASE UBR3; 1.
DR PANTHER; PTHR21497; UBIQUITIN LIGASE E3 ALPHA-RELATED; 1.
DR Pfam; PF18995; PRT6_C; 1.
DR Pfam; PF02207; zf-UBR; 1.
DR SMART; SM00396; ZnF_UBR1; 1.
DR PROSITE; PS51157; ZF_UBR; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU366018};
KW Reference proteome {ECO:0000313|Proteomes:UP000054843};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU366018};
KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU366018};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366018};
KW Zinc-finger {ECO:0000256|RuleBase:RU366018}.
FT DOMAIN 106..177
FT /note="UBR-type"
FT /evidence="ECO:0000259|PROSITE:PS51157"
FT ZN_FING 106..177
FT /note="UBR-type"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00508"
FT REGION 975..998
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1042..1065
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1084..1134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1171..1199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1088..1134
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1179..1198
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2002 AA; 230600 MW; D554CC3BA0927393 CRC64;
MDTSSNVDAM WLEKPLLDWM NDRKEASAMI FQEFCMQMKR LSRRPEAEEQ YMIMRAQMDE
FCLNLLSFFE RTSTRLPNHN WCVQLIAGGM SKEDFLAEVR KYDYSALCGY IWLSGHVAYR
CRTCQINPSM SICASCFHNG DHKNHDFNMF RSQTGGACDC GDKTVMRSEG FCSHHGDGIL
ASATKPPEIL ICSAEQIIPY IIWRLLICYR DYSYVLSGMP GMEHNDFSGI IMLLQTYSEA
GEPLKRIIAE CLLDEKLYKK FCTDLTTSTH NYLVQMTGHY DKAVDYMKPI YSFHEVEHYD
CQDLYGLLVH RNMMQELVFW LVKHHFPQSL TNFLLSLLPN ADFKIAFAET YAKHYPHLAK
ILSQELNPDV VANRVVHISV QILSNNELAY FLVDKCSFLR LVMFSLNSMV RYCLVASSIQ
SSASEVHLVV DCRAPIMEKH SYWAIALDLH SLLGHKDLAL MMLRDPVVLD LWITMALFFQ
GMNPNHRMLD KHVDYEMKTY SAAFTAEVDM FSSTLWSFLQ HLTSEDSFDC ILMVLQAASG
ALQDWLRNMY YGSYPWQLPP LELCFHYPLH RIYVAFLQRG LRLGMELVIP SESFLRMLML
HPLRIQVGRC EIASGMWVRN GASIRYQSYL YSQSHFCSSF IDLDLYLMQL CMQKCNPEWF
VLTVFDCFHT ASWFSFSPSA RNPYLRPDWI AAQVDSALQL IATLYVWIVN LVSSEEDILK
LEVMTLLSGG EYTYSQITDH LPERGSMCEF TALFDKTLNE LADYKQPTFD RSGQMTQGTY
VMKDWVWEEL YDPLYVMSRT SSLKDFRNSF NLFNQYATRT RNLSASESNA LWIPLRFPKK
TLPPNDSIWR VLHSRSLHSI IYTILHRAVF RTACISDFTL SLCIFFLELA IRNPPEESTI
QKSSVGTSVL DLQYCSWFPS NSIELNLSHR IFHVICSDDF VQILQRQDDR RENVISFYDL
IHLWEIDSQT ASEDSANSTL SDTAPDSTDG QQLSSSIPFI DSSVHGNVYT DAEEFRSRMI
AMLHPTVRTR LFSQLTGNER AVQNDAEMNG TNDNDGRISV SNGEQPTQGM QFCRAIELLS
YGCSSGERTE DEEEEEGDEE EEGEGEEEEE EEEEEGEGAE GDEEGEGETE GDEQDIEELL
RRIQQVQRIR LLQRRQQQQQ QQQQQQQQQQ QQQQQQHEQE QEGVDEVDGQ RSSKEKKSSH
GMITALSFDG HSILSLLVVL HFRMLKEKLK NGKVTRPLYY QYPTERPSDE DEEPPIGDGI
YFMERVLNRA YFINQSCRDE IHRICEQISK PASEILQASA PPEDRKQLEA KRKQEILAKF
QEKQGNIMKD WMIKHAELMK DDVEEQKDKE ETPYTCVVCN QHCGPSMENP ISLLLHMQST
NVVSKQRLDV KSRADMGIIS ADRGELTLKF HKMLSDKFGP VATFNAFGSG LERGVFIQSC
GHCIHATCHA DYLKALKSDQ HIVSSFSLHL NNNELLCPLC RRVCNAIAPI LEFSPPESCR
RDETSFDQSL ADIAAMLNIP PTNVPETLAL CGTFVGQLWT KCNQPQKYLA NSSELSVLQN
FVVSTARITT ELNATIRRSP NAIFPHCNEI IMRVLAIVSS NMAVGDLQEY AIQWWQLSGG
HLMDSCYRLL KVPMVPILLQ DAYILLIRYV LFILPKAEAV KSRFHAICRV VWNVLITQIL
LSELLVASDE EYQSFPEVKS GVRTKKFSFM HNNIDGLLSC LASRIGELRN LPMSNSIYKN
VDNCVRLDDM NAVVKLVQAK MLPYLRYAAF LKCVVCKLPL PDDDQFEDFY FLKRFCLDLS
ETDQEMVIFW QSNYVAETLY RQLAQLIQYF KNNPNDGELM VMSVPRVWLQ PKLIDLPDSY
QTLFEKYHFA VCDLCETHSE DSLLCLLCNE FLCFRSSCCR NAEGLSEAEW HSCKCNAGTG
VFLSIEESSV IITRDKRYAE WGFLYLDSHG EEDRGLKRGK PLKLCRDRLA FLVEQWLTHG
FDYFIKRWSW ITRNPNVYSA TT
//
