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Database: UniProt
Entry: A0A0W0GJ35_9CHLR
LinkDB: A0A0W0GJ35_9CHLR
Original site: A0A0W0GJ35_9CHLR 
ID   A0A0W0GJ35_9CHLR        Unreviewed;       589 AA.
AC   A0A0W0GJ35;
DT   16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT   16-MAR-2016, sequence version 1.
DT   11-DEC-2019, entry version 24.
DE   RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993443};
DE            EC=2.7.7.- {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993444};
GN   Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974};
GN   ORFNames=DEALK_13930 {ECO:0000313|EMBL:KTB48547.1};
OS   Dehalogenimonas alkenigignens.
OC   Bacteria; Chloroflexi; Dehalococcoidia; Dehalogenimonas.
OX   NCBI_TaxID=1217799 {ECO:0000313|EMBL:KTB48547.1, ECO:0000313|Proteomes:UP000053947};
RN   [1] {ECO:0000313|EMBL:KTB48547.1, ECO:0000313|Proteomes:UP000053947}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IP3-3 {ECO:0000313|EMBL:KTB48547.1,
RC   ECO:0000313|Proteomes:UP000053947};
RA   Key T.A., Richmond D.P., Bowman K.S., Cho Y.-J., Chun J., da Costa M.S.,
RA   Rainey F.A., Moe W.M.;
RT   "Genome sequence of the organohalide-respiring Dehalogenimonas
RT   alkenigignens type strain (IP3-3T).";
RL   Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC       molecules used as primers for DNA polymerase during DNA replication.
CC       {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811,
CC       ECO:0000256|SAAS:SAAS00709340}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|SAAS:SAAS00709317};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|PIRNR:PIRNR002811,
CC         ECO:0000256|PIRSR:PIRSR002811-1};
CC       Note=Binds 1 zinc ion per monomer. {ECO:0000256|PIRNR:PIRNR002811,
CC       ECO:0000256|PIRSR:PIRSR002811-1};
CC   -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC       Rule:MF_00974}.
CC   -!- SIMILARITY: Belongs to the DnaG primase family. {ECO:0000256|HAMAP-
CC       Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811,
CC       ECO:0000256|SAAS:SAAS00709351}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|HAMAP-Rule:MF_00974}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KTB48547.1}.
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DR   EMBL; LFDV01000002; KTB48547.1; -; Genomic_DNA.
DR   RefSeq; WP_058439514.1; NZ_QEFQ01000001.1.
DR   EnsemblBacteria; KTB48547; KTB48547; DEALK_13930.
DR   PATRIC; fig|1217799.6.peg.1440; -.
DR   OrthoDB; 1071997at2; -.
DR   Proteomes; UP000053947; Unassembled WGS sequence.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR   Gene3D; 3.90.580.10; -; 1.
DR   Gene3D; 3.90.980.10; -; 1.
DR   HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR   InterPro; IPR013264; DNA_primase_core_N.
DR   InterPro; IPR037068; DNA_primase_core_N_sf.
DR   InterPro; IPR019475; DNA_primase_DnaB-bd.
DR   InterPro; IPR006295; DNA_primase_DnaG.
DR   InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR   InterPro; IPR030846; DnaG_bac.
DR   InterPro; IPR034151; TOPRIM_DnaG_bac.
DR   InterPro; IPR006171; TOPRIM_domain.
DR   InterPro; IPR002694; Znf_CHC2.
DR   Pfam; PF10410; DnaB_bind; 1.
DR   Pfam; PF13662; Toprim_4; 1.
DR   Pfam; PF08275; Toprim_N; 1.
DR   Pfam; PF01807; zf-CHC2; 1.
DR   PIRSF; PIRSF002811; DnaG; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   SMART; SM00400; ZnF_CHCC; 1.
DR   TIGRFAMs; TIGR01391; dnaG; 1.
DR   PROSITE; PS50880; TOPRIM; 1.
PE   3: Inferred from homology;
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993445};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709369};
KW   DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709327};
KW   Magnesium {ECO:0000256|SAAS:SAAS00709345};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR002811,
KW   ECO:0000256|PIRSR:PIRSR002811-1, ECO:0000256|SAAS:SAAS00709338};
KW   Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709339,
KW   ECO:0000313|EMBL:KTB48547.1};
KW   Primosome {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811,
KW   ECO:0000256|SAAS:SAAS00709304};
KW   Reference proteome {ECO:0000313|Proteomes:UP000053947};
KW   Transcription {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709341};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993442,
KW   ECO:0000313|EMBL:KTB48547.1};
KW   Zinc {ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|PIRSR:PIRSR002811-1,
KW   ECO:0000256|SAAS:SAAS00709300};
KW   Zinc-finger {ECO:0000256|PIRSR:PIRSR002811-1,
KW   ECO:0000256|SAAS:SAAS00709301}.
FT   DOMAIN          252..333
FT                   /note="Toprim"
FT                   /evidence="ECO:0000259|PROSITE:PS50880"
FT   ZN_FING         34..59
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR002811-1"
SQ   SEQUENCE   589 AA;  64437 MW;  CFA6391FE995C333 CRC64;
     MDAVNEIKNR ADIVELISKY TVLTRSGKTM RGLCPFHSEK HGSFFVYPES GTWHCFGACA
     SGGDVFSFIM KMEGLNFSEA LERLAETYGV VLPSRSEAST HNEHRNELVA VNAAAALYFH
     DLLLNSPSAA KARAYLEKRG VNAGSIEDFQ IGYALPDWQA LKDHLNSAGY ADDILVEVGL
     LGQADSGRLY DRFRHQIIFP ISDARGKIAG FGARVLDDSQ PKYLNSPETP LFSKSGALYG
     INLAAQTIRA NDAAIIVEGY FDVIISHQFG FKNTIGSMGT AVNEQQVAII KKLSPNLIIA
     FDADAAGEAA TARCVSFENL LGREIKVVLA PPGKDPDDTI RANPSAWETS LGNALPIVDF
     VFDMAASKFD LRTARGKAGL TERLLPVVAE IKDPVRRGHY LAILAGKVGT SPSELQYSLS
     RQALLRSVGK SSTLPPHDSP SGLHGNACED YLLAIILKHP SIVENQRIPD PEIFQSLENK
     EIIQLLLKYP GAESIRDKLD SFTRSRFDLI AGIHLDDKNV ALKFQECCRR LEKEYLKTLL
     IKIQEAMKGN NSEGSADGSP ELTEHISAIA GKLAELDAQD AIRRRAPKR
//
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