ID A0A0W4ZVG5_PNEJ7 Unreviewed; 748 AA.
AC A0A0W4ZVG5;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 08-NOV-2023, entry version 32.
DE RecName: Full=cysteine--tRNA ligase {ECO:0000256|ARBA:ARBA00012832};
DE EC=6.1.1.16 {ECO:0000256|ARBA:ARBA00012832};
DE AltName: Full=Cysteinyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00031499};
GN ORFNames=T551_00442 {ECO:0000313|EMBL:KTW32352.1};
OS Pneumocystis jirovecii (strain RU7) (Human pneumocystis pneumonia agent).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Pneumocystomycetes; Pneumocystaceae; Pneumocystis.
OX NCBI_TaxID=1408657 {ECO:0000313|EMBL:KTW32352.1, ECO:0000313|Proteomes:UP000053447};
RN [1] {ECO:0000313|Proteomes:UP000053447}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RU7 {ECO:0000313|Proteomes:UP000053447};
RX PubMed=26899007; DOI=10.1038/ncomms10740;
RA Ma L., Chen Z., Huang D.W., Kutty G., Ishihara M., Wang H., Abouelleil A.,
RA Bishop L., Davey E., Deng R., Deng X., Fan L., Fantoni G., Fitzgerald M.,
RA Gogineni E., Goldberg J.M., Handley G., Hu X., Huber C., Jiao X., Jones K.,
RA Levin J.Z., Liu Y., Macdonald P., Melnikov A., Raley C., Sassi M.,
RA Sherman B.T., Song X., Sykes S., Tran B., Walsh L., Xia Y., Yang J.,
RA Young S., Zeng Q., Zheng X., Stephens R., Nusbaum C., Birren B.W.,
RA Azadi P., Lempicki R.A., Cuomo C.A., Kovacs J.A.;
RT "Genome analysis of three Pneumocystis species reveals adaptation
RT mechanisms to life exclusively in mammalian hosts.";
RL Nat. Commun. 7:10740-10740(2016).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KTW32352.1}.
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DR EMBL; LFWA01000002; KTW32352.1; -; Genomic_DNA.
DR RefSeq; XP_018231044.1; XM_018372708.1.
DR AlphaFoldDB; A0A0W4ZVG5; -.
DR STRING; 1408657.A0A0W4ZVG5; -.
DR GeneID; 28938963; -.
DR VEuPathDB; FungiDB:T551_00442; -.
DR eggNOG; KOG2007; Eukaryota.
DR OrthoDB; 2140072at2759; -.
DR Proteomes; UP000053447; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004817; F:cysteine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd00672; CysRS_core; 1.
DR Gene3D; 1.20.120.1910; Cysteine-tRNA ligase, C-terminal anti-codon recognition domain; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR HAMAP; MF_00041; Cys_tRNA_synth; 1.
DR InterPro; IPR015803; Cys-tRNA-ligase.
DR InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00435; cysS; 1.
DR PANTHER; PTHR10890:SF3; CYSTEINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR10890; CYSTEINYL-TRNA SYNTHETASE; 1.
DR Pfam; PF01406; tRNA-synt_1e; 1.
DR PRINTS; PR00983; TRNASYNTHCYS.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:KTW32352.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000053447};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 36..452
FT /note="tRNA synthetases class I catalytic"
FT /evidence="ECO:0000259|Pfam:PF01406"
SQ SEQUENCE 748 AA; 87444 MW; 32A9AB820FEE25D3 CRC64;
MSFFPKEQRH WSPPKPKKSL KKLVVWNSLT REKNEFVPLN GRKITWYCCG PTVYDWSHMG
HARNYVTTDI IRRILQDYFK YDVIFVQNVT DIDDKIIIRA RHNYFYNKYA KTVTEITPEV
YNKVLIAWKN FAITNFPSAP TSLEIFEEWS NSIKDLETFK SDGKLQMNFT ILTNSYEALI
SYKTLSPAIF LEKVKDILVL ELDKELKHTI NDNTIFREIP AYWERKYNED MKALDVLEPS
VIVRVTEFIP KIIEFIKGIM KNGYAYEFKG SILFDTSAFE ANSCHTYAKI EPWNKNNKKL
IIEGEGSLTQ SEGKKVPSDF ALWKASKPGE PAWDSPWGRG RPGWHIECSA MAAEILGSEI
DIHSGGIDLA FPHHDNEIAQ SEGFFDSDQW VNYFFHIGHL HIEGQKMSKS LKNFITIQDA
LKKYTSKQMR LYFLMHQWNL QMDFKESFMS DIKRIETLLS NFFANINALV MEKKMFLKQN
EIITYFFSDL EKELYENLYN AQDALHSALC DNFNTPVALD VVLNLITKTN KYISQAQMDI
NILMITNISK WVSNILGIFG LKDNNFNVID YDKFNDSTIN DHKEIVMPYI KTLSSFRDKI
RNISIVSRSK SNIELSKDIL QLCDKLRDSD LTDLGIQLED RDQGDALIKF VSKEELQQEK
KLKQELLNKK ASLEQEKLLE KILKGKLSPK ELFQNNEYSK WDNQGLPTHD AQGNELTKSK
RKRIAKDWEK QHILHEKYLE WCKKNTQN
//
