ID A0A0W7WWJ2_9ACTN Unreviewed; 5242 AA.
AC A0A0W7WWJ2;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE SubName: Full=Non-ribosomal peptide synthetase {ECO:0000313|EMBL:KUF14928.1};
GN ORFNames=AT728_37090 {ECO:0000313|EMBL:KUF14928.1};
OS Streptomyces silvensis.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1765722 {ECO:0000313|EMBL:KUF14928.1, ECO:0000313|Proteomes:UP000054804};
RN [1] {ECO:0000313|EMBL:KUF14928.1, ECO:0000313|Proteomes:UP000054804}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 53525 {ECO:0000313|EMBL:KUF14928.1,
RC ECO:0000313|Proteomes:UP000054804};
RA Johnston C.W., Li Y., Magarvey N.A.;
RT "Draft genome sequence of Streptomyces silvensis ATCC 53525, a producer of
RT novel hormone antagonists.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUF14928.1}.
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DR EMBL; LOCL01000051; KUF14928.1; -; Genomic_DNA.
DR STRING; 1765722.AT728_37090; -.
DR Proteomes; UP000054804; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProt.
DR GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 2.
DR CDD; cd17646; A_NRPS_AB3403-like; 1.
DR CDD; cd19543; DCL_NRPS; 2.
DR CDD; cd19540; LCL_NRPS-like; 1.
DR Gene3D; 3.30.300.30; -; 4.
DR Gene3D; 3.40.50.980; -; 6.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 6.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 6.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR010060; NRPS_synth.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 4.
DR NCBIfam; TIGR01720; NRPS-para261; 2.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 4.
DR Pfam; PF13193; AMP-binding_C; 4.
DR Pfam; PF00668; Condensation; 6.
DR Pfam; PF00550; PP-binding; 4.
DR SMART; SM00823; PKS_PP; 4.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 4.
DR SUPFAM; SSF47336; ACP-like; 4.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 12.
DR PROSITE; PS00455; AMP_BINDING; 4.
DR PROSITE; PS50075; CARRIER; 4.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 4.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000054804}.
FT DOMAIN 972..1046
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2487..2562
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3576..3650
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 5154..5228
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 2105..2129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3061..3081
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4590..4609
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5242 AA; 557246 MW; 13C68B6CBBC2DA8D CRC64;
MTGAPSGLQD ILPLSPLQQG LYFLSSYDES ALDVYTVQIA FDLEGPLDAP RLRRAARALL
RRHPNLMAAF RQRKNGDPVT LIPHDVELPW RDSDVSALPT AEGERRALRI ADEDRAERFD
LATPPLIRFT AIRLAEHRHR LLFTHHHLLL DGWSTARVIQ ELFTLYAADG GPHDLPQVRP
YRDYLAWAAA RDTAADERAW QRALAGLEGP TVVAPAGLTA KEPATFDVPL PADLGAALTA
TARRLDVTVP TAVQTLWSLV LAELTGRQDI VSGTTVSGRP AELGGVESMV GLFINTLPVR
VALRSDETLA ALLARVSREQ SALLAHHHLG LARVQQLADL GGPLFDTLCV FENYLVETGA
AQADGAQDGP AEFAGLRVTA VQGKDATHYP LTLVAAPAAD GGLALRLRHH PDAFDTARVR
RLADRLVRAV EEFTADPHRP LAGIDLLTAP ERERVLKTFN SDVTDVTART LPELFEERAA
AHADLTAVAD GARTLTYAEL NERANAVAHH LIAHGVGPED VVGVALPRGA DVYVAQLAVG
KAGGVFAPLD PAYPADRVTA LVADAGIRTV LTRSGVDTAP WAGTATVLHV DAPPAAGPRH
NPTDTDRRAP LRLDHGAYLI YTSGSTGAPK GVLVAHRPLA DLIAWAHACF GTGPGDRVTQ
FASPSFDVTF CELANSLFSG ATLVVVDEER RAGEPLAEFL AEAALTLAVI PPSVVASLPR
AAELPADLTL IVGTEALPGE VIRTWAARHR LFNAYGPTEA VVNSATWRVP HDWTGGPVPI
GPPDVNKRAY VLDEALRPVA PGTLGELYIA GTGLARGYLG RPGITADRFV ADPFGPAGTR
MYRTGDLARW NEAGELEYAG RTDHQLKIRG FRVEPGEVEA RLTAHPRIAQ AVVTDLADER
GVRRLVAHVV PVPGDTGPGH DALREWAGRT LPDHMVPTAL VTLDALPLTR ANKIDRTALP
APDLAPAADR VAPRDETEKE LAALFTEILG VPDAGAHDSF FALGGDSISS IQLVGAAKRR
GMVLRPRDVF EARTVEALAV RVRTADQRAS DAVRATATAT GSVPLTPILR WLVERGGPLS
DYHQSTVLHT PPGTRLAHLS AGLGQLLDHH DVLRARLTGD TLEIPDAGGT DPAALITRVE
AAGVADADLT GVLRPLAERV AQELDAFAGV MVRAVWLDPG ADRGGLLALL IHHAVVDGVS
WRILTEDLAA ACDAAVRGGR AELPAVGTPW AAWAAGLRDA AGTAERTARA EDWQALLDAP
RTSLAAPLDP ALDTLGTVRT VAVRLDPDIT GPLLTEVPER YHAGPDEVLL TALASALTTW
RGRSGPVLVD VEGHGRVEDA VPGAELSRTV GWFTSVHPVR LDLTGQDAPG TGRTGTALKR
IKEQLRAAGQ DGIGYGMLRH LNPETAPRLA GAAGAGAEIG YNYLGRFAAA GTGASAGDGP
APWSPALAGS LGGGADPALP ASHTLMLNAS TVDGPEGPVL QASFSFPERL LDESAVRTLA
DLWLAALRTL AADCATTGGG HTPSDFPLVR VDQQEIEALE ARHPLSDLLP LSPLQEGLYF
LSGIGAEGEG PDVYTTQTVL EIEGRLDAER LRRAGRRLLD RHPNLRAGFH DRPDAAPLQA
VPAAVDIPFA EFDLRAGVPG AAGTTPGLPE RDRARQLLAE ERGRGFDLAD PPLIRFTLVR
LGEDRQLFAL TSHHILLDGW SGPLLVQDLL ALYRGDPGPA PRPYRDHLLW LAGRDRARTQ
ELWRENLAGL EGPTLLVPHA AGMPAGVPER VELPLDDDLV AQLYAFARGR DVTVNTVLQA
AWGLLLGWLT GRDDVVFGIT VSGRPADLDG AQRMIGLFIN TVPTRVRMRP DQPLADLVQD
LAAQQTRLLD HQYEPLAELQ RLAGHRELFD TLVLFENFPV DAAQLRRSEE RAGLVVTGAE
GHDATHYPLV LVALPGSGDR LALALDHRPE LLPTERAETI GGHLRDVLAQ LVTAPHRTTR
ALHPATGDPH RLDGPTRQTT AHGLAGRFLD RAAATPDATA LIADGTAWSY ARLGERVATL
AARLAARGAG PGTPVAVALP RRADLVATLL AVAATGAAYV PVDPEHPAER IAHVLGDARP
LLSVDEDSPV LAPGHEPYPD PPGRRPRPDD AAYIIHTSGS TGTPKGVVIS HRNLANLLDT
MADTLGSGPG GRLLAVTTVS FDIAALELFV PLVTGGTVIL ADRAQVLDPQ LLAALAEDTG
ATHLQATPSL WRGLVEAAPG LLDGLTVLSG GEPLAADLAE RLATDGARLF NLYGPTETTV
WSTAGELHAE GGAPHVGHAV HNTTVRVLDP WLRPVPAGTP GELYIGGTGV ARGYLGRSAL
TASRFVADPY GRPGERIYRT GDVVHWRADG TLRVRGRVDH QLKVRGHRVE PGEIEAVLRT
CPEVGDAVVV GLPDPTGSTR LVGYVTGKGA AAPEPSALRA FVAERLPDYL VPSVLMVLPA
LPLTPNGKVD RAALPAPQTA AAGGGRTPRD PREAVLAELF ADVLGLARVG ADDDFFALGG
HSLLAMRLAN RLRSALGTDV ALRDVFDHPT PAALAAAVLP RGERRAPLLP REHAPGELLP
LSFAQSRLWF LHRLDGPSAT YNLFFVLRLG GALDVRAMRL AVGDLVARHA ILRTVYPDTD
GLPHQLVLDE AAGRAAADPG EPVPVADADL ERALHAQVTA AVDIIEEIPL RVRILRTEHD
HVLAVMLHHI AGDEWTMGPL TEDLRAAYLA RTTGTEPDWP PLEVSYADYA VWQREFLGDA
DDPGSPAARQ AAYWRTALAG SPEELALPYD RARPAEEHHD GATVTFAVDP RTHRALRALT
ATTGTSVFMA AQASLAALLA AHGAGTDLPI GTPVAGRTDA ALDDLVGFFV NTLVLRTDLS
GRPTFRELLA RVREIDLAAF DHADLPFEQL VELIAPERTL ARNPLFQVML VFQNVSDDEL
TLPGLDVTPV SADPGVAKLD LAFTLAERPG GAGINGMLTY QTSLFDRETA QALADRYVTL
LAALVAEPDV PVHRVQALTA DERARVLAAG TGAEHALPPT TLPALLAERY ARTPDALALV
ADEEDEENEG GEGEKGAPGA DPLVLDNAAF DRRVGALAAV LRERGVEPEV RVAVALPRSA
DLVVALHAVQ RAGGAYVPVD PGYPAERTAH MLSDSAPRVL VTTRAIAAAL DVPDTVEPLL
LDDPATRAEL AATRAELAAA RTAAVHPDLR GEHAAYVIYT SGSTGRPKGA VISHRALVNR
LRWMADTYPL DAADRVLQKT PAAFDVSVWE FFLPMLTGAP LVVLPDGAHR DPVRVAATIT
RHGVTVAHFV PSMLAAFAAE PAAAHCTGLR LLFASGEALP TAVAGAVLRA LPGVALHNLY
GPTEATVDVT AWPAAPTGTS TGVPIGRPVW NTGAHVLDAW LRPVPDGVTG ELYLSGDQLA
RGYLDRPALT AERFVADPFG APGKRLYRTG DLVRRRRDHA LVFVGRADGQ VKLRGLRVEL
GEIEALLGEQ PEVAACAVLL RDDRGGSPLL TGYAVPAAGA APDPDELTAR LARRLPDHMV
PGALLLIDQL PLTPGGKLDR AALPAPDLAA RASGGAPQGI REARLTELFA DLLAVPEVGP
EDSFFALGGD SILSIQLVAR ARKAGLLLTP RDVFEHKTPA ALARAARTTG RATALPAVPA
TGRVPLTPIM RWALDRGPLT GLHQYLYVIA PAEADGENLT AALDLLFDRH PMLRARLVDT
GDGQQALDVP PYTAGHGADA LLHADLTGVD PADRATAIAG HASRAVDELD PERGRMVRAV
WCDLGPDEPG RLLLIIHHLV VDGVSWRILT ADLTAAHRAV LDGTDPPAAH PSTSFRQWAL
GLADAAREPG RQAEAAVWRA RDEALAPALG SRPLDPAVDT QETVAHLERT LPSDTTHALL
TDVPAAVHGT VHDVLLAAAA LAFGRWRAER GEHAFGPDVI AAEGHGREEG VVPGAELSTT
VGWFTSWYPV VLDPGTPPAG GSAARAALIA VKEHLRQGPD NGIGHGLLHR FTDLAGPART
PEAVFNYLGR FTGASGTQEG DGAWTPAPET LPPLRPARNP LPVLFPLEII ASTVDGPEGP
ALATRWSYAT GVLAEHDAHR LLDLWCAALA ELAADRTPPT RTPSDFPLVR LGQDAVDDMQ
RRLPDLTDVW PLAPLQEGFH FLTQLADDEL DVYTMQLTLD LTGAVDAAAM RDAGADLLAR
NANLRTAFLT TADRTAVQAV LAEVAPQWQE SDLRALPRAE RQAALRDLAD DDRARPFDLT
APPLLRMRLV RLADEEYALV VTNHHAVLDG WSVPVLMQEF FQLYGARTGT GTPPPTRRPF
RDHLRWLGEQ DCAAAVDAWR SALDGVDTPT LLTPADPTRT PVLTRHVRHE ADGALHDLLT
ALARDADVTV NTVVQFAWGL LLARHTGRDD VLFGATVSGR PSELEGVEAM TGLFINTLPV
RVDLRAPGSV RAALARLQEN QSRLTAHQHL ALAEVQRIAG VGVLFDTLLV FENYPVDADG
LRRAERSGGL TVTGGRSADA THYPLTLAVV PGRGLTLEYR PDLFTEAEAE ALMRRYLHLL
RELGARPDGP VHRIDALDAG ERERVLGHWS GREAGRRGAG GRGREDAVAA VPAGSAVPAG
SVGSAGSAGS AGETLPGLLA ARIAADPHAP AVVHDATQLT FAELGERSAR LAHELIARGI
GPEDVVALLL PRTEEMLVAL NAVHLAGAAY LPLDPDYPAE RIAFMLGDTA PRLLLTTEDL
RKRSAPQEEQ QGTAVECVAL DDPAVADALA ARPPVLPYDT DRVRPLLPDH PAYVIYTSGS
TGTPKGVVVA HRGLVNLFHS HRGALYEPVV ERTGRRRLHV AHAWSFSFDA SWQPQLWLYH
GHCVHVLDED ALRDPVALHD YLRSHAVDFV EVAPSTLALL EQAGLMDGDR CPLPLLGVGG
EAVPDAQWAR LKRLAGTEAV NLYGPTECTV DAMVARVRDS EKQVVGHPVD GARVHLLDAG
LRPVPPGVPG ELYVAGAGLA RGYLGRPGLT AERFVADPFG SGGRLYRTGD LARWTPGGAV
EYLGRADDQV KIRGFRVELG EIESVVSRRP DVAQAVVVAR EDGGVRRLVG YAVPRPGADL
DARELRAGVA RELPDHLVPA AFVVLDALPV TANAKLDRGR LPAPDFGGAV LGREPEGERE
ELLCAAMARA LGLPAVGADD DFFALGGDSI VSVQLVAALR ADGWRLSPRQ VFLLRTPAAL
AREIGHAGDE PAPGARTAAA GS
//
