ID A0A0W8CIM5_PHYNI Unreviewed; 2425 AA.
AC A0A0W8CIM5;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE SubName: Full=Calpain-5 {ECO:0000313|EMBL:KUF83939.1};
GN ORFNames=AM587_10009421 {ECO:0000313|EMBL:KUF83939.1};
OS Phytophthora nicotianae (Buckeye rot agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=4790 {ECO:0000313|EMBL:KUF83939.1, ECO:0000313|Proteomes:UP000052943};
RN [1] {ECO:0000313|EMBL:KUF83939.1, ECO:0000313|Proteomes:UP000052943}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=race 0 {ECO:0000313|Proteomes:UP000052943};
RA Liu H., Ma X., Yu H., Fang D., Li Y., Wang X., Wang W., Dong Y., Xiao B.;
RT "Genomes and virulence difference between two physiological races of
RT Phytophthora nicotianae.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the peptidase C2 family.
CC {ECO:0000256|ARBA:ARBA00007623}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUF83939.1}.
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DR EMBL; LNFO01003042; KUF83939.1; -; Genomic_DNA.
DR STRING; 4790.A0A0W8CIM5; -.
DR EnsemblProtists; KUF83939; KUF83939; AM587_10009421.
DR OMA; QANGAFF; -.
DR Proteomes; UP000052943; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00044; CysPc; 1.
DR CDD; cd00051; EFh; 1.
DR CDD; cd00201; WW; 1.
DR Gene3D; 2.20.70.10; -; 1.
DR Gene3D; 2.60.120.380; -; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR Gene3D; 1.10.238.10; EF-hand; 2.
DR InterPro; IPR022684; Calpain_cysteine_protease.
DR InterPro; IPR036213; Calpain_III_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR PANTHER; PTHR10183; CALPAIN; 1.
DR PANTHER; PTHR10183:SF379; CALPAIN-A-RELATED; 1.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR Pfam; PF00648; Peptidase_C2; 2.
DR Pfam; PF00397; WW; 1.
DR PRINTS; PR00704; CALPAIN.
DR SMART; SM00230; CysPc; 1.
DR SMART; SM00054; EFh; 3.
DR SMART; SM00456; WW; 1.
DR SUPFAM; SSF49758; Calpain large subunit, middle domain (domain III); 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF51045; WW domain; 1.
DR PROSITE; PS50203; CALPAIN_CAT; 1.
DR PROSITE; PS00018; EF_HAND_1; 3.
DR PROSITE; PS50222; EF_HAND_2; 2.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
DR PROSITE; PS01159; WW_DOMAIN_1; 1.
DR PROSITE; PS50020; WW_DOMAIN_2; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00239};
KW Protease {ECO:0000256|PROSITE-ProRule:PRU00239};
KW Reference proteome {ECO:0000313|Proteomes:UP000052943};
KW Thiol protease {ECO:0000256|PROSITE-ProRule:PRU00239}.
FT DOMAIN 77..430
FT /note="Calpain catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50203"
FT DOMAIN 1106..1141
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 1263..1298
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 2362..2395
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT REGION 2327..2347
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2095..2183
FT /evidence="ECO:0000256|SAM:Coils"
FT ACT_SITE 183
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
FT ACT_SITE 319
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
FT ACT_SITE 344
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
SQ SEQUENCE 2425 AA; 269954 MW; CDD2199CC5D38776 CRC64;
MRSAVKDNGA ADLHTLQEEL KKLGISVVSG RQDTVISVKI KGLAGRALIR GLKLADQLLQ
EKIKHAIKAC TDPATKFFTD PTFGPCSSDP DGAAAICKAG GVIPSKGGSQ HQAKVIGLLQ
RGKIRWERPI YAPDNEEDDI SPQEDEDDIY AMSSSDNVFA SNATLFADGV SSGDVIQGNL
GDCWFLSALS VVATRSDLLA QTFWRRDQHK SKGLFVCKFM KNFVWNYVLI DDRLPVFGFT
DKKPGKPYFA RCRNPNELWV SLIEKAYAKL HGSYEALIGG FIDCALNDLT GMCAEQPPVT
STKEVAVESS AGNGLYFKHA YALVDAADIK TAKGEYVRLV KLRNPWGMGE WTGPWSDSSD
ERAANEDAID KFFKIMKRKV GANADKRVFM TLNPQGRVGA DEISQEEVIE INANDGTFFM
SFDAWMQSFT HFFAGIDFPD SWHGRRSEGS WNEANCGGNT TKSTWINNPH FELILEQRAR
IFASLSQEDP RGSENLKIVP VGFHICSLSP VGNDPNKFEI REPTKKLDAY YRSYKPVDRD
NYMTGKRPEP LPPAIIPGTV IPGIDDDGVP QAAYTFKQAV SVRSVEATME PGRYCIIPSM
YMRTNYGQDK VTGNTNVGNF WISVYSDKPT FRLEGGEKIV EEEEGDEETQ TNGSQSYKNL
NAGATTLTRQ FSSTNSSRKS ETIVATGNAV AAEKRRNFEN EKEELLRQAK RYGIGLRELR
TAFGKTPKVS KAECRQKLQK LGFNVASWDD DKIGLLFGGL EEGPTSGLIQ TDRILNLFTP
DIQEEKMACE LPDMEEDGDT PDSIHREGIL EIELHGASGL AVGEKRQSLI QRKVPIPTPT
FSTSQISLRR KIAAEILERD PSMAWYLRQF AKKSTLLANT HNTSKQNRDG SVRSQLHIPT
PVAAFYELLE KEYAARIISR CDVEDELEYV VPVSRTPSYS KRDGISPFYP SLSSKSAPEW
NLPPTPLNEL ELHKSSRIRD LEAKRSTKLA LLLQHKQQAL GDAEKTASLP RLIGGRKSGS
SHVKCIDCGA RVLVGGRSKT ATSQDIDSCP CNGANCVNVF CSPCYSLLPT KSKLCEECYQ
HEISPEERFG EQLRGVLIEK IGASDQQRSH LEEVFRSFDV DGSGSLSPQE FEKMLQLLNI
QPTLTQRQKA FLVGQFDANG DGEISLIEFK HWILRDHVWV ESVAPTSHKT IPHTAEVMST
VIAPLCEEII DLAYNAYAFS STIGSWSRSS SSTTECQKKT LQSGETGVII FQRVIKLSVT
SGGEANVAEK VFEQVDDDGS GSIDQFEFVA LLSALGIHLN SEDAVLLMNR FGAASTGSGR
ITIEKAAFIE YVGQMSTKTF SRVGDGSLDK ILVKLAEFAA TNPVARESLQ DELQQLHENI
TQKELLRICK NLDEKASLIG GVDELRSLAM NLAFDHEMNA SNTEREGADQ HGPLKSSGDI
LARMLLSESG DLMKLVRTFD VTSTCRELAS HLQIRSGTNS TRTIWKSVFG ADTTDVVHLR
DFISCVLKTG FLIEVQNEPD KLGVATHDRL ITPLLLSLAV DAVRTDCHVL STSPEASPVI
SFNLFRLIVR LQKIKEIELK FDHALTNFMQ LCQGEQQYLV TIALDASRNL VVRAGDPVFK
FSADFELRED EFDYQNLIAQ LGSASFERRQ QETHEVSREL AFAKAPYGLI TSEFQPELNN
SMLTIIERLR AELRCRHHST TSHTSRLHIL SLTMVESEAF VSTLRNLLSL VELPFFWSVS
PRSLVFSIDS EYLDQQSKPS FQQLVADTLS LHGSQLPPPL RALATFVAHT ASSLLVRYEV
VGKYTNVETS WQEFQDFISG HQNAYAVMEL HPQGSVLTTR VARASGSGAV QWNFKTQIKM
DEPKLCDHRI DRPVVFTDTV KVSCIPGSTE NINLIRGDSE GPGHFVVISV RRALPNGSDF
PKPRLYCTAY DPLTSCDYAV EGYPSDWSVD FFDVTVNPSF ESQWQTMLDS MRLGVTITPK
LAITVYNKQP KTDKLIGECE VSIGSAVVQE GHIFEERVAL RPPVMNPTSA CAGVTSLTFR
FDVEKTSSIR EDVVAERRQQ DTGTRVPIKL GAQYLAGEIV TPRTESVPDN GNDRVKELQT
SMTAMEMSKL EAQEQVKQLK AQIQQLSYAS NKTTEDNAER WKRRLEQARQ EQFAAEEQHA
ARLAALQEEI RHLSEETQKQ KNAHREASNL KDCRLSADAS AYDIFSSIRE ILTRRCPERP
YNGLKKALAA EAEVPGKVTF AVLNDVLGDF GLALSSDQRS TLANLFDPEM VGRVNIEDFF
INLCGDAEAY EKVLTSRLQL STTPESSSPV AEDVAVVEYP EPIVFRPTPP AQRPVSPTAS
PARTSSVRSK MSWQDMKKFL VLNLPEGWET RFTEKGRPYF CNHANRSTQW KHPRPEIETI
FSEWVQANGA FFHRKSVSAR PCKQK
//
