ID A0A0X1T2A0_PSEAA Unreviewed; 4579 AA.
AC A0A0X1T2A0;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE SubName: Full=Non-ribosomal peptide synthetase {ECO:0000313|EMBL:AMB86151.1};
GN ORFNames=AWM79_12925 {ECO:0000313|EMBL:AMB86151.1};
OS Pseudomonas agarici.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=46677 {ECO:0000313|EMBL:AMB86151.1, ECO:0000313|Proteomes:UP000063229};
RN [1] {ECO:0000313|EMBL:AMB86151.1, ECO:0000313|Proteomes:UP000063229}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCPPB 2472 {ECO:0000313|EMBL:AMB86151.1,
RC ECO:0000313|Proteomes:UP000063229};
RA McClelland M., Jain A., Saraogi P., Mendelson R., Westerman R.,
RA SanMiguel P., Csonka L.;
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR EMBL; CP014135; AMB86151.1; -; Genomic_DNA.
DR RefSeq; WP_060783018.1; NZ_CP014135.1.
DR STRING; 46677.AWM79_12925; -.
DR Proteomes; UP000063229; Chromosome.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR CDD; cd17646; A_NRPS_AB3403-like; 1.
DR CDD; cd17649; A_NRPS_PvdJ-like; 1.
DR CDD; cd12115; A_NRPS_Sfm_like; 1.
DR CDD; cd19543; DCL_NRPS; 2.
DR CDD; cd19534; E_NRPS; 3.
DR CDD; cd19531; LCL_NRPS-like; 1.
DR Gene3D; 3.30.300.30; -; 3.
DR Gene3D; 3.40.50.980; -; 6.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 6.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 6.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR010060; NRPS_synth.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 3.
DR NCBIfam; TIGR01720; NRPS-para261; 3.
DR PANTHER; PTHR45398; ENZYME, PUTATIVE (JCVI)-RELATED; 1.
DR PANTHER; PTHR45398:SF1; ENZYME, PUTATIVE (JCVI)-RELATED; 1.
DR Pfam; PF00501; AMP-binding; 3.
DR Pfam; PF13193; AMP-binding_C; 3.
DR Pfam; PF00668; Condensation; 6.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 3.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 12.
DR PROSITE; PS00455; AMP_BINDING; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000063229}.
FT DOMAIN 1024..1098
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2534..2608
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4046..4123
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 4579 AA; 508400 MW; 35B9E7A946E5C939 CRC64;
MDKQVAARIA KRFITLTLDK RTLYLQKMLE EGVSPAHLPI PEVKSSFEAL PLSFAQQRQW
FLWKLDPQSS AYHIPTALRL RGALDVASLE RSFNALIARH ESLRTRFVED QGRTLQVIEP
ELKLSLSVRQ WSGATACDEA IAAFVGEQSQ EVFDLTQAPL LRVTLLRLAV DDHVLVLTQH
HIVSDGWSMQ VMVQELVELY GAFSQGRQVE LPVLSIQYAD YAIWQRHWME AGEQARQLEY
WKATLGAAQT VLELPTDHPR PAVQSLRGAT LALTLEARLV NGIRQLAQQE NVTLFMVLLA
SFQVLMHRYS GQREILIGVP SANRNRVETE RLIGFFVNTQ VFKAEVDGTA PFRQLLAACK
QSALGAQAHQ DLPFEQLVEA LQLERSLSHN PLFQVMYNHQ SAARQVPRAA AGPASLLDVE
VLAWENHTAQ LDLTLDTFES ADGLSASLTY ATDLFSAATV ERMARHWQNL LRAVTADPAG
RIGELPMLDD SERLQRLEQW NATRTAYPLE RTVHSLIEQQ ASATPDAVAL VFAGESLSYR
QLDGRANHLA HQLIAQGVGP DVLVGIAVER SLEMVVGLLA ILKAGGAYVP LDPEYPEDRL
AYMIADSSIS LLLTQRRLLA RLPLPDTVAT LCLDELEADC VCATPPPVAL HPENLAYVIY
TSGSTGKPKG AANRHSALTN RLCWMQQAYG LGSDDAVLQK TPFSFDVSVW EFFWPLLTGA
RLVIAEPGDH RDPARLVGLI NQQRVTTLHF VPSMLQVFLQ DENVALCTGL KRIVCSGEAL
QVDTQQQVFA KLPDTALYNL YGPTEAAIDV THWTCREEGR DSVPIGEPIA NLQTYVLSHE
LALVPNGVMG ELYLGGEGLA RGYHRRAGLT AERFVVSPLG DGQRLYRTGD LVRQRANGII
EYVGRIDHQV KVRGLRIELG EIEARLLEQA SVREAVVLAI EGSGGLQLVG YVVPADDSLA
GADPQAQGAL RDALKLALKG SLPDYMVPAH WLFLAQLPLS PNGKLERKAL PKPDVSQLQQ
TYVAPRNELQ RRIATVWQEV LKVERVGLSD NFFELGGDSI ISIQVVSRAR QAGIRFTPKD
LFQHQTVQGL ATVAQVGDGG LVIEQGPTVG ATPLLPFQRL FFATVTVERH HWNQSVLLKP
SRPLDPAQLE QVLRALIVQH DVLRLRFSQE VDRTVSAWTA TYRTLREQEA IWQQSPVLHQ
ADAADVAALE QLGEEAQRSL DLGNGPLLRA VLVTMADGSQ RLLLVIHHLV VDGVSWRILF
EDLQTAYQQL QAGEALALPA KSSAFKRWAE RLQAYARDKA QQQVLPYWLE QFQGVVADLP
CDRPGAALQN RYADSVQSRL DTTFTQQLLQ QAPAAYRTQI NDLLLTALAR VIGRWTRQAS
VLVQLEGHGR EDLFNDIDLT RTLGWFTSLY PLKLTAQGTP GSAIKAIKEQ VRAIPDKGLS
FGALRYLGDE AAREALAGLP LPRITFNYLG QFDGSFSEAQ GALFVPSGES RGAEQSPEAP
LGNWLTVNSQ VYAGELSVSW SFSREMFDVQ TLQVLADEYV QELKELIAHC CESPAQGVTP
SDFPLARLEQ AQLDNLDVPA GQIENIYPLS PMQHGLLFHS LYEPHSSHYI NQMRLDVEGL
DASRFQQAWQ AAMDAHDILR SRFVWQGEFA EPMQLVHKHL AVPFTVRDWR AHAQLDLDLE
ALAQSEQQDF DLSRAPLLRL QLVRTGENRH HLIYTHHHIL MDGWSHSQLM GEVLQRYHGQ
TPIAHPGHYR DYIAWLQRQD VAVSESFWKA QLAPLQEPTR LAGAFVPAGA GGQGDLRQLV
DVDTTRQLSE FARQQKITVN TLLQASWLLL LQRYTGLGTV CFGATVSGRP ADLKGVEQQI
GLFINTLPVI ASPCAQQSVG EWLQAVQAGN VALREHEHTP LFDVQRWAGL GNDALFDSLL
VFENYPVAEA LQQEAADDLL FGRIDNHEQT NYPLTLSVTL SETLSLHYNY AQTHFSDAAV
EQISRHLLIL LVALSRSAQT PIGELGLLSA DERRLQLEAW NANELAYPRE LCIHQQIDAQ
ASSQPQAIAL TVGAQHWSYE QLNRQANQLA HRLIESGVGP EVRVGVAMSR SAQMVVALLA
VLKAGGTYVP LDPDYPAERV AYMLKDSGAK VLLTERELLA GLTVPQAEIL VLEATGTAFA
EYPAQTPDSA VTAQNLAYVI YTSGSTGQPK GVAITHRNVA ALIQWSRQVY SQDDLQGVLA
STSICFDLSV WELFVTLARG GSIILARNAL ELPDLPARDQ VRLINTVPSA IAALQRAGQI
PASVRIINLA GEPLKQALVD SLYGLASVEH VYDLYGPSED TTYSTWTRRE AGGLANIGRP
LANTQSYLLD SQLQPVPPGV AAELYLAGAG ITRGYLFRPG LTAEKFVPNP FGESGERLYR
TGDLCRYRRD GVLEYVGRLD HQVKVRGFRI ELGEIEARLL AQGQLQEVAV LAVEGGAGSQ
LVAYAVPLGG RVDGESAQVL RETLKEALSA SLPDYMVPAH WLFLEALPLT PNGKLDRKAL
PAPDVTQAQK AYVQPQGELE QRLAAVWEDV LQVGRIGLSD NFFELGGDSI LSIQVVSRAR
RAGIFFTPKD LFQHQTVRDL ARIATLDGGA LAIDQGPMEG VMPLLPIHQM FFEEDIPQRH
HWNQSVMLTS ALALDPLRLE QALRALILHH DTLRLGFVQQ VDGWVATYRS VAEQQSFWQR
SAVMTQATAQ DGEQLQQLAN ALQASLDLQK GPLLRALLVN MADGGQRLLL VIHHLAVDGV
SWRILFEDLQ TAYQQLVAEQ AVALPLKSSS LKSWAQHLQA RARSTAQEPE LIYWHTQLQG
VSSDLPARNP QGRLQRRHAT TVHSALDASL TRQLLQQAPA AYRTQINDLL LTALARVIGR
WTGQTDSLIQ LEGHGREDLF GTLDLTRSVG WFTSIFPVRL SSDADLARSI KQVKEQLRAI
PDKGIGFGAL RYLGDERAQS LLKALPVPRI TFNYLGQFDG SFDGAQGALF SPCGDHRGAE
QSAEAPLANW LTLNGQVFAG EFGMSWTFSR EMFDEATIQH LARAYENELA LLIEHCCAPD
HQGCTPSDFP LSPLTQQQLD ELVVDCQGVV DIYPLSPMQE GMLFHAVADS ESSFYVNQLS
LPVQGLDPQR FCEAWQAAVA RHDILRTSFH WQGGASKPYQ IVHASVPFDL SELDWRGIAV
DDSKLQEQAT VERDKGFDLA CAPLQRLLLI RLDDHCYQVI WTSHHILMDG WSSSRLFGEV
LQHYAKGEVA QPPARYRDFI HWLQRQDQSA LQAFWQARLQ PVDTPTSLAQ AVHPRHVADL
AGHQALYSNW DPRQTSRLQA YCRELRITLN TLIQGAWLLV LQRYTGQRTV SFGATVAGRP
ESLPGAEHML GLFINTLPVI QTLEPATPLA DWLRQLQGYN VDIRDYAHVP LADIQRWSGL
GGQALFDSII VFENYPIDER LHEDATQELA FGKTQSHDVT NFPMDLAVHL TDRLSIEYLF
LRNCFAPSAV ERIRQTLEDT VEAMLRQPGV ALGQLQRLPT AQRQELLAWS HQCAPDTRPQ
LLPALIARHA LERPLAIAVQ CGGQTLTHAQ LDRRSNQLAH ALIARGAGPE GVIGVGLERS
VDTIVALLAV LKSGAAYVPL DIDYPADRLA YMIEDSGMLL LISRSELLER FANVDRLPCL
ELDGFDGELW SEQTPLSGAT AQSLAYLVYT SGSTGRPKGV AVPQGPLSMH CQAIARLYEM
DSSTRELHFM SFAFDGAHER WLSTLFSGGR LVLRDGNLWT PEETFAALIR HEVTIACFPP
AYLKQLADYA QASGLQPPAV RIYCFGGDAV PEQTFEQVKR SLRPQYITNG YGPTETVVTP
MLWKAASTDD CEAAYAPIGR VVGERSLYLL DDVLEPLPMG FAGELYIGGQ GVARGYHQRP
ALTAERFVPN PFDPSGERLY RTGDLVRLRT DGVLDYVGRI DQQVKVRGFR IELGEIEACL
RQQAGVNDAL VVARDSASGK QLIGYVVCAS ASAVSPSLKQ ALREVLPEYM VPAQIIGLSE
FPVTPNGKID RKALPEPRFD SDGYLAPRSD AQRWLAQIWA EVLHVERVGI QDNFFELGGD
SILSLQVVSR VRNHPDLQMD LKLRDLMRCQ TIERLFDGRD EPVAVARDDR SHLISEGLFN
LIPIQEWFFA QDMAEPHHFN QALLLRPRQS LDSAALEAAL NRLILQHDAL RLRFRRENGR
WYQYYAPAQQ CIPAQGLLWR CEAADGEALK ELANRVQRSL HLEDGPLFRA VQIDMADGQS
RLLLVIHHLV IDTVSWRILL QDMQSSYESL LQGLEPELAM RTSSYQAWSE RLRSYGQSLV
DSEQAYWLEQ LDRPGHDLPC DNPRGKNQVR HVATAHLELS AQATTDLLKK VPLVYQTQIN
DVLLAALSRV LCRWSEQSSV LVQFEGHGRE DLFDEIELSR TVGWFTSMFP VRLAPGVEAG
FADSIRAVQA QLAALPNKGM GYGALRYLSD ASVVERLSRL PQARVTFNYL GQFDQSFDDN
ALFVPADESS GDSYSLQAEL GNWLEIVGQV FDGKLALRCL YSRRRYRPET VETLMANFQA
ELEALIAHCL EQAPALSLA
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