ID A0A0X1T5S2_PSEAA Unreviewed; 1116 AA.
AC A0A0X1T5S2;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Potassium transporter KefA {ECO:0000313|EMBL:AMB87447.1};
GN ORFNames=AWM79_19960 {ECO:0000313|EMBL:AMB87447.1};
OS Pseudomonas agarici.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=46677 {ECO:0000313|EMBL:AMB87447.1, ECO:0000313|Proteomes:UP000063229};
RN [1] {ECO:0000313|EMBL:AMB87447.1, ECO:0000313|Proteomes:UP000063229}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCPPB 2472 {ECO:0000313|EMBL:AMB87447.1,
RC ECO:0000313|Proteomes:UP000063229};
RA McClelland M., Jain A., Saraogi P., Mendelson R., Westerman R.,
RA SanMiguel P., Csonka L.;
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family.
CC {ECO:0000256|ARBA:ARBA00008017}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP014135; AMB87447.1; -; Genomic_DNA.
DR RefSeq; WP_017130102.1; NZ_JACAQG010000057.1.
DR AlphaFoldDB; A0A0X1T5S2; -.
DR STRING; 46677.AWM79_19960; -.
DR OrthoDB; 9799209at2; -.
DR Proteomes; UP000063229; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR Gene3D; 1.10.287.1260; -; 1.
DR Gene3D; 2.30.30.60; -; 1.
DR Gene3D; 3.30.70.100; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR049142; MS_channel_1st.
DR InterPro; IPR049278; MS_channel_C.
DR InterPro; IPR023408; MscS_beta-dom_sf.
DR InterPro; IPR006685; MscS_channel_2nd.
DR InterPro; IPR011066; MscS_channel_C_sf.
DR InterPro; IPR006686; MscS_channel_CS.
DR InterPro; IPR011014; MscS_channel_TM-2.
DR InterPro; IPR025692; MscS_IM_dom1.
DR InterPro; IPR024393; MscS_porin.
DR PANTHER; PTHR30347:SF8; MECHANOSENSITIVE CHANNEL MSCK; 1.
DR PANTHER; PTHR30347; POTASSIUM CHANNEL RELATED; 1.
DR Pfam; PF21088; MS_channel_1st; 1.
DR Pfam; PF00924; MS_channel_2nd; 1.
DR Pfam; PF21082; MS_channel_3rd; 1.
DR Pfam; PF12795; MscS_porin; 1.
DR Pfam; PF12794; MscS_TM; 1.
DR SUPFAM; SSF82689; Mechanosensitive channel protein MscS (YggB), C-terminal domain; 1.
DR SUPFAM; SSF82861; Mechanosensitive channel protein MscS (YggB), transmembrane region; 1.
DR SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
DR PROSITE; PS01246; UPF0003; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000063229};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..1116
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5035254257"
FT TRANSMEM 487..506
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 536..558
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 578..601
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 613..631
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 643..664
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 676..700
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 712..731
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 779..802
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 822..843
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 864..885
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 897..925
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 37..271
FT /note="Mechanosensitive ion channel MscS porin"
FT /evidence="ECO:0000259|Pfam:PF12795"
FT DOMAIN 491..809
FT /note="Mechanosensitive ion channel inner membrane"
FT /evidence="ECO:0000259|Pfam:PF12794"
FT DOMAIN 870..911
FT /note="Mechanosensitive ion channel transmembrane helices
FT 2/3"
FT /evidence="ECO:0000259|Pfam:PF21088"
FT DOMAIN 913..978
FT /note="Mechanosensitive ion channel MscS"
FT /evidence="ECO:0000259|Pfam:PF00924"
FT DOMAIN 986..1069
FT /note="Mechanosensitive ion channel MscS C-terminal"
FT /evidence="ECO:0000259|Pfam:PF21082"
FT REGION 1093..1116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 221..251
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 284..311
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1116 AA; 124479 MW; 0D1889916F55C450 CRC64;
MSFMRSLFVT TLLGLSLAVG ALQAAEPPTS GSVQQSLDKI AERKLPDADQ KALQLVLQQT
LSWLSSADDY DKRLSATKQQ VAEAPRQTNE NQRELARLKV TKVVPVAQRY ANQSVPQLEQ
LLSERTTQQG ELQKALADAN SLIITAQTRP ERAQSEISSS QTRIQQINTT LKLGKDNGKT
LSGDQRNQLN AELASINALI LLRRQELAGN SQLQDLGNSQ HDLLMEKTAR LEQEIQDMQT
LINQKRLAQS QKTVTQQSIE AQKAGDSSLL ATESATNLKL SDYLLKSTDR LNELTQQNLR
AKQQLDSVTQ SDQALDEQIS VLKGSLLLSK ILYKQKQALP RLQLDKNLAD EIADIRLYQF
EVNQQRELIN SPSTYVDNLL ATQPPEQLNP QLRRTLLDLA VTRGDLLERL NRELSALLNE
SITLQLNQKQ LLDTAQALRA TLDEQMFWIP SNKPLDLEWL EGVPSRLQKQ LNTLPWASSL
SELSEGLLQR PLLFLPLLLL IGALLWRRRH LYAQLSKVHK DIGHFKRDSQ WHTPQAILVN
VLLALPIALG LALCGYALQI DARGQNANLG AALLQMSQAW LVFYTAYRIL APGGVAELHF
HWEKPQVEFL QGWIRRLGFV VMALVAVVAV AEHQPAALAD DVLGIAVVLT CYALMAWLLS
RLLLRSPNHH NASLFRKLVG VVFTALPIAL FVAVCFGYYY TALKLSDRLI NTLYLLMFWL
VIEATFVRGL AVAARRLAYQ RALSKRQAAK DAGDGEPTVE EPALDIAQVN QQSLRLIRLI
LLGGFVAALY WVWSDLITVF SYLDNITLYE YTSGTGATMS MVPITIGNVI GALAIVGITF
ALARNLPGLL EVLVLSKLEL AQGSAYATTT LLSYVIAGIG FVSTLSTLGV SWDKLQWLVA
ALSVGLGFGM QEIFANFISG IMILFERPVR IGDTITIGTL SGTVSKIRIR ATTITDFDRK
DIIVPNKTFI TGQLINWSLT DTVTRVTLKL GVDYGSDLDR VKELLLKAAR DNPRVLKEPE
PHVYFLNFGE STLDHELRMH VRDLGDRNPV IDEVNRFINC EFKKEHINIS FRQMEVYLKN
LHGQEYKLVP VEGETSSLAP KPPVEPDQQS PLNKLD
//
