ID A0A0X3V8R8_9ACTN Unreviewed; 1063 AA.
AC A0A0X3V8R8;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=alpha-amylase {ECO:0000256|ARBA:ARBA00012595};
DE EC=3.2.1.1 {ECO:0000256|ARBA:ARBA00012595};
GN ORFNames=ADL15_05450 {ECO:0000313|EMBL:KUL41181.1};
OS Actinoplanes awajinensis subsp. mycoplanecinus.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Actinoplanes.
OX NCBI_TaxID=135947 {ECO:0000313|EMBL:KUL41181.1, ECO:0000313|Proteomes:UP000053244};
RN [1] {ECO:0000313|EMBL:KUL41181.1, ECO:0000313|Proteomes:UP000053244}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL B-16712 {ECO:0000313|EMBL:KUL41181.1,
RC ECO:0000313|Proteomes:UP000053244};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in
CC polysaccharides containing three or more (1->4)-alpha-linked D-
CC glucose units.; EC=3.2.1.1; Evidence={ECO:0000256|ARBA:ARBA00000548};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUL41181.1}.
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DR EMBL; LLZH01000017; KUL41181.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0X3V8R8; -.
DR OrthoDB; 9805159at2; -.
DR Proteomes; UP000053244; Unassembled WGS sequence.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0051060; F:pullulanase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd10315; CBM41_pullulanase; 2.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 2.60.40.1110; -; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 2.60.40.1130; Rab geranylgeranyltransferase alpha-subunit, insert domain; 1.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR005323; CBM41_pullulanase.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011839; Pullul_strch.
DR InterPro; IPR024561; Pullul_strch_C.
DR InterPro; IPR040671; Pullulanase_N2.
DR NCBIfam; TIGR02103; pullul_strch; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF03714; PUD; 2.
DR Pfam; PF11852; Pullul_strch_C; 1.
DR Pfam; PF17967; Pullulanase_N2; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 2.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 2.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000053244}.
FT DOMAIN 11..105
FT /note="Pullulanase carbohydrate-binding module 41"
FT /evidence="ECO:0000259|Pfam:PF03714"
FT DOMAIN 118..208
FT /note="Pullulanase carbohydrate-binding module 41"
FT /evidence="ECO:0000259|Pfam:PF03714"
FT DOMAIN 253..328
FT /note="Pullulanase N2"
FT /evidence="ECO:0000259|Pfam:PF17967"
FT DOMAIN 338..422
FT /note="Glycoside hydrolase family 13 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02922"
FT DOMAIN 900..1060
FT /note="Alpha-1,6-glucosidases pullulanase-type C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11852"
SQ SEQUENCE 1063 AA; 116080 MW; 65AD8A79CC9DFB6E CRC64;
MLRVTTRPHW LIVHYHRPDG DYSGWALHVW GDVAPDQAAA FPAGQAFDGE DAHGRFAWVR
LSAEAADVGF VVVHSSGVKD VDLDRRVDVR TTTAILLHAG DRRVHQVPAA PPPDPGFAVL
HYRRPDGDYS GWGLHVWEGV PIKGKTLWGT PLEPTDFDGY GAVFRVPLKP DAVGLRYVLH
QSERKDLPDD QRLDLTVTRE VWLLAGTTEP VRPDLGTLGP ELDPARSFAV FVDRTTIALP
EWFAARAATF ELLTAETTLA LTPRPGGLFQ AQSRRFPHLR AYRAFAVREL GDRVLGGLLR
DRLLIEGRDT AGDLVARTAV QIAGVLDDLY LEAADADLGL TLDVDRPQLA VWAPTARTVE
LELSREPGSE PRILPMDRDD LTGVWSIPVK RKWLGRFYRY RVETWHPAAQ RVVTTSVTDP
YSVSLAADST HSQLVDLDAA ELKPPGWDDA AKPAAVAPAR MQIAEVSVRD FSIGDGSLPA
AERGTYLAFT HAGSDGMRHL RSLAEAGLTH VHLLPVNDFG TVPDHRADQA QPACDLVTLP
PDSPEQQRAV MAVADEDGYN WGYDPWHWTT PEGSYATDPA GTARILQMRS AVAALNATGL
RVVLDVVYNH TMRDGLDRFS VLDRIVPGYY HRLLADGSTA ESTCCPNTAP EHMMMGRLVI
DSLVTWARAY RIDGFRFDLM GHHPRANILE ARIALDHRVE HGRDICLYGE GWNFGEVAYD
ARFAQATQVN MAGTGIGSFN DRMRDAARGG GAFGDPGVPG FATGLGQRTP GFVHDRIKVG
LAGGLATYRF VTHDGVERSG AQVDYNGSPC GYASAPGETV NYVDAHDNEI LYDAMAFKLP
PGTRPADRAR MQVLALSLVV LGQGAGFMAL GSERLRSKSL DRNSFNSGDW FNQIRWDPGH
GNGFGTGLPP FDDNREVWGH ARPLLADPAL IPSAEVINLA AERYRELLRI RRSSPVFGLP
TADEVQRRLT FPLGGPGESP GVIVMGLDGT GLDERWRRLV VVFNATDEPT CQVVPGEGPL
RLHPELTASV DPVLRGASAV TKLDGAELTV PARQVAVFVA DLA
//
