ID A0A0X7B972_9FLAO Unreviewed; 1509 AA.
AC A0A0X7B972;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN Name=dnaE_2 {ECO:0000313|EMBL:KVV13692.1};
GN ORFNames=AP058_03057 {ECO:0000313|EMBL:KVV13692.1};
OS Flavobacterium sp. TAB 87.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=1729581 {ECO:0000313|EMBL:KVV13692.1, ECO:0000313|Proteomes:UP000059935};
RN [1] {ECO:0000313|EMBL:KVV13692.1, ECO:0000313|Proteomes:UP000059935}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TAB87 {ECO:0000313|EMBL:KVV13692.1,
RC ECO:0000313|Proteomes:UP000059935};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KVV13692.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LLWK01000038; KVV13692.1; -; Genomic_DNA.
DR RefSeq; WP_066312681.1; NZ_LLWK01000038.1.
DR STRING; 1729581.AP058_03057; -.
DR PATRIC; fig|1729581.3.peg.3154; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000059935; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd06127; DEDDh; 1.
DR CDD; cd04485; DnaE_OBF; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000313|EMBL:KVV13692.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000059935};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:KVV13692.1}.
FT DOMAIN 1..192
FT /note="Exonuclease"
FT /evidence="ECO:0000259|SMART:SM00479"
FT DOMAIN 262..350
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1509 AA; 171290 MW; 2715C9A0DEA275AF CRC64;
MYLIFDTETT GLPKNWNAPI TDSSNWPRCI QIAWQLHDEM GNLIEHQDYL VKPDGFNIPY
DAERIHGIST ELAQQDGISL TEVLEKFNIA LSKSKFIVGQ NLGFDVNIMG AELYRMGVNS
PMSSMPVLDT CTEVTASLLK LPGGRGGRFK LPTLTELHEY LFNVPFSEAH NATADVEATT
RCFLELIRRE IFTKEELDVP ANYFEAFQNK NPKEIRLIGL KHINLKAASD KIRAQLKALD
SVATETVISD SEKHDLRAAK FAHLHNHTQF SVLQSTIAIP KLVAATAKDN LPAVAMTDTG
NMMGAFHFVS SVMNHNKASA SKNKTLVEAG EEPTETEIKP IVGCEFNICE NHLDKTKKDN
GYQIVLLAKN KKGYHNLAKM ASIAYVSGFY YVPRIDRSVV EKYKEDIMVL SGNLYGEIPS
KILNIGEIQA EEALIWWKEQ FGADFYLEIM RHNQEDENRV NKTLIEFSQK HKVKLIATNN
TFYLNKEDAN AHDILLCVKD GEKQATPIGR GRGYRYGLPN QEYYYKSEKE MKKIFSDLPD
AIINIQEIVD KVEIYSLYRD VLLPKYDIPA EFVNPEDEKD NGVRGENAYL RHLTMVGAEK
RYGEISEAIQ ERLDFELLTI SNSGYPGYFL IVQDFIAKAR EMDVSVGPGR GSAAGSAVAY
CLKITNIDPI KYDLLFERFL NPDRVSMPDI DIDFDDEGRG RVMDYVINKY GANQVAQIIT
YGKMATKSAI RDTARVLDLP LFEADRIAKL IPPMMPSKWN LARFISEKEE DVKKALRSDE
FDNIKELIAI ANEDGLAGET IQQAKILEGS MRNTGIHACG VIITPSDITD FVPVTTAKDS
ALYVTQFDNS VAESAGLLKM DFLGLKTLTL IKDTVKLVKY RTGIQLDPDA FPIDDAETYE
LFQRGETVGI FQYESPGMQK YMKDLKPTVF GDLIAMNALY RPGPIEYIPS FVRRKNGEEE
IKYDLDACEE YLGETYGITV YQEQVMLLSQ SLAGFSKGDA DVLRKAMGKK QKEVLDKMKP
KFVAQAVEKG HDAKILEKIW KDWEAFASYA FNKSHSTCYA WIAYQTAYLK AHYPAEYMAA
VLSNNMNDIK QVSFFMEECK RMGLQVLGPC VNESFYKFTV NDDYAVRFGM GAIKGVGANA
VKTIVDTRKE VKYKSIFDLA KRIDLRAANK KAIENLALAG GFDAFVGTTR AQYFHDEGDG
VTFYEKAIRY GVKYQENENS AQVSLFGESS DVQIAEPVVP PCEDWSTMEK LAKEKEVVGI
YISGHPLDDF RFEMKYFCNA RLEALRNLEQ YVTKSLTFAG IINNVQHRVA KNGKGWAMFN
LEGYDESFEF RIFGEEYLKF RHFLIQNNFT FIKVLVKEGW TNQDTGKKSD PRIQFTEVKQ
LQDVLENFAK KLVLLLNIKD LDADFIHHLN RLFSEYKGDN TVTFEIMELE KIKKLVETTT
EYSEEEQEVF EDENEDSDTL AQTKVAQLTE VEEINVVTKL TMPSRKLKVK ISTELLIELE
KMQINFKLN
//
