ID A0A0X8FD47_9LACT Unreviewed; 824 AA.
AC A0A0X8FD47;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=AWM72_05920 {ECO:0000313|EMBL:AMB94327.1};
OS Aerococcus sanguinicola.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Aerococcaceae; Aerococcus.
OX NCBI_TaxID=119206 {ECO:0000313|EMBL:AMB94327.1, ECO:0000313|Proteomes:UP000069912};
RN [1] {ECO:0000313|EMBL:AMB94327.1, ECO:0000313|Proteomes:UP000069912}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG43001 {ECO:0000313|EMBL:AMB94327.1,
RC ECO:0000313|Proteomes:UP000069912};
RX PubMed=27103727;
RA Carkaci D., Dargis R., Nielsen X.C., Skovgaard O., Fuursted K.,
RA Christensen J.J.;
RT "Complete Genome Sequences of Aerococcus christensenii CCUG 28831T,
RT Aerococcus sanguinicola CCUG 43001T, Aerococcus urinae CCUG 36881T,
RT Aerococcus urinaeequi CCUG 28094T, Aerococcus urinaehominis CCUG 42038 BT,
RT and Aerococcus viridans CCUG 4311T.";
RL Genome Announc. 4:0-0(2016).
RN [2] {ECO:0000313|Proteomes:UP000069912}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG43001 {ECO:0000313|Proteomes:UP000069912};
RA Carkaci D., Dargis R., Nielsen X.C., Skovgaard O., Fuursted K.,
RA Christensen J.J.;
RT "Six Aerococcus type strain genome sequencing and assembly using PacBio and
RT Illumina Hiseq.";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP014160; AMB94327.1; -; Genomic_DNA.
DR RefSeq; WP_067974760.1; NZ_CP014160.1.
DR AlphaFoldDB; A0A0X8FD47; -.
DR GeneID; 69592848; -.
DR KEGG; asan:AWM72_05920; -.
DR Proteomes; UP000069912; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 2.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000069912}.
FT DOMAIN 9..288
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 289..626
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 751..775
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 30..37
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 824 AA; 93364 MW; AC16E0E761DAF1E3 CRC64;
MASREDLIQG LNDKQKEAVQ CTEGPLLIMA GAGSGKTRVL THRMAYILSE KDVRPWNLLA
ITFTNKAAKE MRERVDQLIG QGAEAMWVAT FHSMCVRILR REAEAIGLSR SFTIADPAEQ
QTLIKQVMKD LNFDTTKFKP RMILGKISDA KNNLQTPDQY ADTHSGLIED IVAKCYVAYQ
KQLLAAQSLD FDDLIMYTVR LFEEQKEVLR YYQQKFQYIH VDEYQDTNEA QYRLVKLLAA
YFKNICVVGD ADQSIYGWRG ANMENILNFE KDYPEAKVVL LEQNYRSTKT ILAAANNVIG
RNQKRKEKDL WTDNDQGEKI HYYRAQTEQD ESYFVIEEIK KALQKGYQYK DVAVLYRTNA
QSRVVEENLV KANLPYRIVG GLKFYDRKEI KDILAYLRLL ANPQDNLSFT RVVNVPKRGV
GPGSLDKLRH FASDQGLTLL QAAGHVDYAP ISGKAAKSLK NFAQMLADLN QERAELSIRQ
LTEKLLDQSG YQEDLENQKT LEAQTRLENI QEFLSVADEF DKRWREEAGD RQAAAAADDQ
GQPVQVEAIQ LPEGPGPSAQ KQEAISLFGD DTLDRLLDEE DLAAPELEED RLLAFLTDLS
LVSDTGEEEE SASGQVTLMT LHAAKGLEFP IVFMIGMEEG LFPLSRAAQD PDELEEERRL
AYVGITRAEE KLYLSNSYSR MLYGKREAHA PSRFIEEIDA EHLDQSAKQA TSFSFDFGQS
QARKAGSYRN YLKGKQQERA GAQTYYDRQG KEAKRSVFDK PQGQSKSADQ ETAWRVGDKA
QHKKWGLGTV VKVTGEGNDQ ELDIAFKAQG IKRLLAAFAP IEKV
//
