ID A0A0X8X978_9SPHI Unreviewed; 1173 AA.
AC A0A0X8X978;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 24-JAN-2024, entry version 34.
DE SubName: Full=Nitrate reductase {ECO:0000313|EMBL:BAU55719.1, ECO:0000313|EMBL:MBB3058495.1};
DE EC=1.7.7.2 {ECO:0000313|EMBL:MBB3058495.1};
DE EC=1.7.99.4 {ECO:0000313|EMBL:BAU55719.1};
GN Name=narB {ECO:0000313|EMBL:BAU55719.1};
GN ORFNames=FHS11_004949 {ECO:0000313|EMBL:MBB3058495.1}, MgSA37_03911
GN {ECO:0000313|EMBL:BAU55719.1};
OS Mucilaginibacter gotjawali.
OC Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC Sphingobacteriaceae; Mucilaginibacter.
OX NCBI_TaxID=1550579 {ECO:0000313|EMBL:BAU55719.1, ECO:0000313|Proteomes:UP000218263};
RN [1] {ECO:0000313|EMBL:BAU55719.1, ECO:0000313|Proteomes:UP000218263}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SA3-7 {ECO:0000313|EMBL:BAU55719.1,
RC ECO:0000313|Proteomes:UP000218263};
RA Lee J.S., Lee K.C., Kim K.K., Lee B.W.;
RT "Genome sequence of Mucilaginibacter gotjawali.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:MBB3058495.1, ECO:0000313|Proteomes:UP000539265}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CECT 8628 {ECO:0000313|EMBL:MBB3058495.1,
RC ECO:0000313|Proteomes:UP000539265};
RA Whitman W.;
RT "Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the genomes of
RT soil and plant-associated and newly described type strains.";
RL Submitted (AUG-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Nitrogen metabolism. {ECO:0000256|ARBA:ARBA00004909}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. NasA/NapA/NarB subfamily.
CC {ECO:0000256|ARBA:ARBA00008747}.
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DR EMBL; AP017313; BAU55719.1; -; Genomic_DNA.
DR EMBL; JACHWX010000021; MBB3058495.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0X8X978; -.
DR KEGG; mgot:MgSA37_03911; -.
DR OrthoDB; 9792592at2; -.
DR Proteomes; UP000218263; Chromosome.
DR Proteomes; UP000539265; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd02791; MopB_CT_Nitrate-R-NapA-like; 1.
DR CDD; cd02754; MopB_Nitrate-R-NapA-like; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.390.30; -; 1.
DR Gene3D; 3.40.50.740; -; 1.
DR Gene3D; 2.20.25.90; ADC-like domains; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.40.228.10; Dimethylsulfoxide Reductase, domain 2; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR041957; CT_Nitrate-R-NapA-like.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR016156; FAD/NAD-linked_Rdtase_dimer_sf.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR041575; Rubredoxin_C.
DR PANTHER; PTHR43105:SF9; NITRATE REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G15190)-RELATED; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF04879; Molybdop_Fe4S4; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR Pfam; PF18267; Rubredoxin_C; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00411; PNDRDTASEI.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 2.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:BAU55719.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000218263}.
FT DOMAIN 8..64
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 1173 AA; 129777 MW; D4D2B2694DE1EA4B CRC64;
MPVKKQQINT STSTCCYCGV GCGVLVHKDK SGNVLIEGNK EHPVNKGMLC SKGLNLHYTV
NDKSDRLLYP QMRYNKNMPM QRVSWDEALD RTAAVFKTFI NKYGPDSVAF YASGQCLTEE
YYVINKLMKG FIGSNNIDTN SRLCMSSAVA AYKMALGEDT VPVCYDDIEL ADCFYVTGAN
PAWCHPILWR RVEAHKAANP KTKIIVVDPR VTDTCGIADL HLQINPGTDI TLNHAIGRAL
IENGDIDLDF VTRHAEGFEQ YSAMVFKRSM ADAARHCGVS EADIRLAARY IAEAKGFISM
WTMGLNQSST GVNKNLSLIN LNLITGHIGK PGSGPLSLTG QPNAMGGREV GGLANLLPAH
RNLDNPLHRE EVQKFWGGSV INPKPGLTAT EMFEALENGS LKAIWILCTN PLTSLPNVRL
AEAALKKAKF VVVQEVSNKP ESLAFADVIL PAAAWAEKEG TMTNSERRIS YLNKIIEPPG
EALPDAEIIC RFARKMGYKG FDFEDPAAIY AEHARLTTKT NIDISSLTYE ILKEKGTVQW
PYKKKSAEEG TIRLFTDKRF YTLSKRAIIH PVPDANTSEQ PDGDFPFILT TGRIRDQWHT
MSKTGKVNKL NQHLPQSFME IHPDDASTLG INDGDITVIT SRRGKVQVKA KISTQIKQGV
VFVPMHWGKI LGNDLNRVNN LTSDQVDPIS KEPDFKFCAV NVVKFKKPFQ RIVVIGAGAG
AYGFVKSYRE LNPDDEITIF SKENFPFYNR VMLPDYISGE QKWEQLVKMT DEDEPGYKIK
LLRGVSVEKI DRSNKQVTDS RGIKTSYDIL LVATGSRASV PKNVPSLPGI FTMRSRVDAD
NFKKHMPNDA HVVIVGGGLL GLEMAASLRE MGVKITIIQR TSSFLNRQLD ALGSQLLHEE
MADQGCDIYY DDEVQLYYGR SKLTGIGLKS GRKINCDAMI LAIGTTPNME IAKDCGLDCR
RGVTVNERLQ TSDPDVFAIG EIAEFNGTLY GITAAAEQQA QVVAGYLNGD IASYYKGSLF
MNIIKIHGFD LCSIGIPECP DDKDYEEVVF IDKAKRYYKK CIIHQDRLVG AILIGDKSEL
LEFRDLIANK TELSEKRLQL LRSGNKADPV LGKLVCSCNN VGSENIRKKI ESGCNTMTDL
CKTTGAGTGC GSCRPEVKRI LDEALKSMVS INN
//
