ID A0A101CML4_9ACTN Unreviewed; 515 AA.
AC A0A101CML4;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=Nickel transporter {ECO:0000313|EMBL:KUJ58971.1};
GN ORFNames=ADL25_01795 {ECO:0000313|EMBL:KUJ58971.1};
OS Streptomyces sp. NRRL F-5122.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1609098 {ECO:0000313|EMBL:KUJ58971.1, ECO:0000313|Proteomes:UP000054048};
RN [1] {ECO:0000313|Proteomes:UP000054048}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL F-5122 {ECO:0000313|Proteomes:UP000054048};
RA Ju K.-S., Doroghazi J.R., Metcalf W.W.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Efflux system for nickel and cobalt.
CC {ECO:0000256|ARBA:ARBA00002510}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUJ58971.1}.
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DR EMBL; LMWH01000001; KUJ58971.1; -; Genomic_DNA.
DR RefSeq; WP_059125767.1; NZ_LMWH01000001.1.
DR AlphaFoldDB; A0A101CML4; -.
DR OrthoDB; 271709at2; -.
DR Proteomes; UP000054048; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR PANTHER; PTHR40659; NICKEL/COBALT EFFLUX SYSTEM RCNA; 1.
DR PANTHER; PTHR40659:SF1; NICKEL_COBALT EFFLUX SYSTEM RCNA; 1.
DR Pfam; PF03824; NicO; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cobalt {ECO:0000256|ARBA:ARBA00023285};
KW Cobalt transport {ECO:0000256|ARBA:ARBA00022426};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Nickel {ECO:0000256|ARBA:ARBA00022596};
KW Nickel transport {ECO:0000256|ARBA:ARBA00023112};
KW Reference proteome {ECO:0000313|Proteomes:UP000054048};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT SIGNAL 1..30
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 31..515
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5007095080"
FT TRANSMEM 239..261
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 285..311
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 408..430
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 436..464
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 485..507
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 348..400
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 365..389
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 515 AA; 51921 MW; 401D6FB97B8893AC CRC64;
MSLRRLVASG AAALTAASAL VLIPSGPASA HPLGNFTVNR YDGLVAAPGQ LRVDHVEDLA
EIPATQAESD IAGTGLTGWA RKRCAAAAEG SRLTVGGRPV TLTVGTSSAR SRPGQAGLST
LRVECRLTAP LPRTGTAGIA FRSAGASTGP GWREITARGD RTTLIASNVP KSSVSHELTA
YPVELLSSPA DTVTASLRVR PGGPALAGEQ GDAPAASVLP RGADRWTRAL DDLVARHDLT
VGFAALALII AIGLGAMHAL APGHGKTLMA ATAAARGSRA RLKDVLPLAA SVTITHTLGV
VALGLLVTAG SAATPSVIAW LGLASGILVT LAGASLVRRA WLSRRHGHLG TDRPGPAPGH
LGHTHRTHGH GEGHQHGDHG HHHDPGVPHT HGGVTHTHPT APTLRGTILL GFAGGMVPSP
SAVVVLVGAA ALGQAWFGLL LVVAYGIGLA LTLTAAGVAV VRLGSGMKRV LERRPRWTAG
PAAALVRRTA PLGSAFVVVA LGAGLVFKGA ASALG
//
